Rational Design of a Novel DNA Polymerase From Clostridium thermocellum to Improve LAMP Detection Performance.

Loop-mediated isothermal amplification (LAMP) is a detection method widely used in pathogen detection and clinical diagnosis. Nevertheless, it is highly constrained by thermal stability, catalytic activity, and resistance to inhibitors of Bst DNA polymerase. In this study, a novel DNA polymerase was characterized from Clostridium thermocellum, exhibiting potential in LAMP detection.

Supplemental effects of in a low-fish meal diet for at varying temperatures: growth performance, innate immunity and gut bacterial community.

This study examined the effects of on the growth performance, innate immunity, and gut microbiota of under different water temperature conditions. Feeding regimens included a 20% fishmeal diet (control), a low-fish meal (LFM) diet with 10% fishmeal and an LFM diet supplemented with 0.03% .

Tomato sprayed monocalcium phosphate had production-phytoremediation dual function with high soil Cd extraction and safer fruit production.

In order to make use of the large biomass of tomato plant to fulfill the purpose of remediating-while-producing, two commercial tomato varieties, 'Baiguoqiangfeng' (BG) and 'Ouguan' (OG) were grown in Cd contaminated acidic soil to compare their performance on Cd phytoextraction, and monocalcium phosphate (Ca) was foliar applied to reduce their fruit Cd concentration. The results showed that the BG was a more Cd tolerant variety, comparing with OG, it suffered lighter tissue peroxidation and photosynthesis obstacle, owning weaker amino acid metabolism, secondary metabolism and stress signal transduction under Cd stress. The Ca application reduced its ABA level but increased the GSH, IAA, ZR and GA3 level, and enhanced its lysine degradation, tyrosine metabolism, alanine, asparagine and glutamate metabolism, plant hormone signal transduction and phenylpropanoid biosynthesis under Cd stress.

Rocaglamide reprograms glucose metabolism in erythroleukemic cells via c-MYC transcriptional regulation of TXNIP and HK2.

Ethnopharmacological Relevance: The theory of traditional Chinese medicine (TCM) views leukemia as an imbalance between cell growth and death mainly caused by blood stasis. Medicinal plants Aglaia Lour. (family Meliaceae) are traditionally used as folk medicine in China.

Safety evaluation of alpha-glycerylphosphorylcholine as a novel food.

To evaluate the safety of alpha-glycerylphosphorylcholine (α-GPC) as a novel food, the study of acute oral toxicity, subchronic toxicity, teratogenic toxicity and genotoxicity were conducted. In acute oral toxicity, no toxic effects were observed in rats of both genders administrated 10.0 g/kg BW α-GPC.

Computing hematopoiesis plasticity in response to genetic mutations and environmental stimulations.

Cell plasticity (CP), describing a dynamic cell state, plays a crucial role in maintaining homeostasis during organ morphogenesis, regeneration, and trauma-to-repair biological process. Single-cell-omics datasets provide an unprecedented resource to empower CP analysis. Hematopoiesis offers fertile opportunities to develop quantitative methods for understanding CP.

A distinct immune landscape in anti-synthetase syndrome profiled by a single-cell genomic study.

Objectives: The objective of this study was to profile the transcriptional profiles of peripheral blood mononuclear cells (PBMCs) and their immune repertoires affected by anti-synthetase syndrome (ASS) at the single-cell level.

Methods: We performed single-cell RNA sequencing (scRNA-seq) analysis of PBMCs and bulk RNA sequencing for patients with ASS (N=3) and patients with anti-melanoma differentiation-associated gene 5-positive dermatomyositis (MDA5 DM, N=3) along with healthy controls (HCs, N=4). As ASS and MDA5 DM have similar organ involvements, MDA5 DM was used as a disease control.

Dynamic covalent and noncovalent assembly of -nitrosocumene in organic solvents and water.

-Nitrosocumene (-NC) exhibits dynamic N,N bonding, interchanging monomer and /-azodioxide dimer structures, the extent of which depends on the environment. As a solid, -NC is a Z-dimer; in organic solvent, the monomer is favored; and in water, dimers are favored. A supramolecular assembly of -NC is observed as a separate species by NMR in water, shown to be a novel nanometer-sized aggregate containing ∼2000 molecules.

Characterisation of a new virulent phage isolated from Hainan Island with potential against multidrug-resistant Pseudomonas aeruginosa infections.

Multidrug-resistant (MDR) Pseudomonas aeruginosa is a serious life-threatening pathogen. The rise in P. aeruginosa resistance rates has renewed interest in phages as an alternative therapeutic approach for treating bacterial infections.

Preparation and Characterization of Hydroxylated Recombinant Collagen by Incorporating Proline and Hydroxyproline in Proline-Deficient .

Collagen possesses distinctive chemical properties and biological functions due to its unique triple helix structure. However, recombinant collagen expressed in without post-translational modifications such as hydroxylation lacks full function since hydroxylation is considered to be critical to the stability of the collagen triple-helix at body temperature. Here, a proline-deficient strain was constructed and employed to prepare hydroxylated recombinant collagens by incorporating proline (Pro) and hydroxyproline (Hyp) from the culture medium.

Uncovering seed vigor responsive miRNA in hybrid wheat and its parents by deep sequencing.

Background: Two-line hybrid wheat technology system is one way to harness wheat heterosis both domestically and internationally. Seed vigor is a crucial parameter for assessing seed quality, as enhanced seed vigor can lead to yield increments of over 20% to a certain extent. MicroRNAs (miRNAs) were known to participate in the development and vigor of seed in plants, but its impact on seed vigor in two-line hybrid wheat remains poorly elucidated.

Examining the controversies in venous thromboembolism prophylaxis for vascular surgery patients: A critical review.

Background: Venous thromboembolism (VTE) is a significant concern in vascular surgery due to its potentially severe consequences. Effective prophylactic measures are essential to minimize the risks associated with VTE. However, considerable controversy remains regarding the optimal strategies for VTE prevention in patients undergoing vascular procedures.

Luminescence properties of SrGaGeO:Bi phosphors and temperature sensing characteristics of co-doped Sm.

As one of the fundamental physical quantities, temperature is extremely important in various fields. In order to study the temperature sensing characteristics of dual-emitting center phosphors, Bi-doped and Bi/Sm-doped SrGaGeO phosphors were synthesized by high-temperature solid-phase method. Under 312 nm excitation, the SrGaGeO:Bi phosphor exhibits a blue broadband emission corresponding to the P → S transition of Bi ions.

Transcranial alternating current stimulation for older adults with cognitive impairment: A bibliometric and knowledge map analysis.

As the population ages, cognitive impairment leading to dementia and related disorders presents an increasingly significant societal burden. Transcranial alternating current stimulation emerges as a potential noninvasive treatment, yet remains an area of ongoing research. Using the Science Citation Index Expanded within the Web of Science Core Collection database, we identified 144 relevant articles spanning from 1965 to December 1st, 2023.

A natural acylphloroglucinol exerts anti-erythroleukemia effects via targeting STAT3 and p38-MAPK, and inhibiting PI3K/AKT/mTOR signaling pathway.

Erythroleukemia, a subtype of acute myeloid leukemia (AML), is a life-threatening malignancy that affects the blood and bone marrow. Despite the availability of clinical treatments, the complex pathogenesis of the disease and the severe side effects of chemotherapy continue to impede therapeutic progress in leukemia. In this study, we investigated the antitumor activity of L76, an acylphloroglucinol compound derived from Callistemon salignus DC.

The genetic diversity and evolution analysis of the Hainan melioidosis outbreak strains.

Melioidosis is a zoonotic disease, with its outbreaks being rare and indicative of an unusual concurrence of extreme climate and natural environmental factors. An outbreak of melioidosis cases emerged in Hainan following Typhoon "Dianmu" from October to December 2021, presenting an opportunity to identify the environmental sources of infection for these cases due to its nature as a well-defined point-source cluster. To investigate the relationship between the occurrence of these melioidosis cases and the environment, we extracted the entire genome of 25 clinical strains and conducted MLST typing, followed by whole genome sequencing and analysis of molecular genetic information for four ST46 genotypes from these strains.

TMJ-105, an extract of , induced G2/M phase arrest and apoptosis via the JAK2/STAT3 axis and MAPKs signaling pathway in leukemia HEL cells.

Leukemia is a malignant tumor of the hematologic system. Studies have shown that cernuumolide J (TMJ-105), an extract of , has anti-cancer effects, but the underlying mechanism is unclear. In this study, we investigated the effect of TMJ-105 on the proliferation of human leukemia HEL cells and its molecular mechanism.