Optimization of Protoplast Preparation Conditions in and Transcriptomic Analysis Throughout the Process.

Protoplasts are essential tools for genetic manipulation and functional genomics research in fungi. This study systematically optimized protoplast preparation conditions and examined transcriptional changes throughout the preparation and regeneration processes to elucidate the molecular mechanisms underlying the formation and regeneration of protoplasts in . The results indicated an optimal protoplast yield of 5.

Associations of C-reactive protein to lymphocyte ratio and metabolic-dysfunction-associated steatotic liver disease: evidence from NHANES 2017-2018.

Background: This study aimed to investigate the association between Metabolic-dysfunction-associated steatotic liver disease(MASLD)and C-reactive protein/lymphocyte ratio (CLR).

Methods: MASLD was defined as a Controlled Attenuation Parameter (CAP ≥ 274dB/m) and CLR = C-reactive protein/lymphocyte. A multifactor linear regression model was used to test the relationship between MASLD and CLR.

Effectiveness of home-based exercise for functional rehabilitation in older adults after hip fracture surgery: A systematic review and meta-analysis of randomized controlled trials.

This systematic review and meta-analysis was performed to assess effectiveness of home-based exercise compared with control interventions for functional rehabilitation in elderly patients after hip fracture surgery. Comprehensive literature search was performed on PubMed, EMBASE, Web of Science, Cochrane library, and Clinicaltrails.gov to identify eligible randomized controlled trials (RCTs).

Correction: Fabrication of yeast β-glucan/sodium alginate/γ-polyglutamic acid composite particles for hemostasis and wound healing.

Correction for 'Fabrication of yeast β-glucan/sodium alginate/γ-polyglutamic acid composite particles for hemostasis and wound healing' by Qinglin Zou , , 2024, , 2394-2407, https://doi.org/10.1039/d3bm02068a.

Injectable and self-healing carboxymethyl chitosan/carboxymethyl cellulose/marine snail peptide hydrogel for infected wound healing.

Treatment of bacterial infected full-thickness wounds remains a great challenge in clinic. Herein, a HYP hydrogel was prepared using carboxymethyl chitosan, dialdehyde carboxymethyl cellulose, and marine snail peptide (Tyr-Ile-Ala-Glu-Asp-Ala-Glu-Arg) as starting materials. The marine snail peptide with good antioxidant activity could remove the reactive oxygen species in wound sites, thereby alleviating the excessive inflammatory response.

The coupling effect and ecological risk assessment of iron, manganese, and arsenic in the water environment of the central Yangtze River Basin, China.

Excessive heavy metal in drinking water are harmful to human body. Groundwater was still the drinking water resource in most of rural areas in the central of the Yangtze River Basin. Heavy metals of Fe, Mn, and As in the low plain region of the Yangtze River Basin significantly exceeded the standard, resulting in 16.

Carboxymethylated yeast β-glucan: Biological activity screening in zebrafish, sprayable hydrogel preparation, and wound healing study in diabetic mice.

Yeast β-glucan exhibits dramatic potential as wound healing regent owing to its various biological properties including immunomodulatory, anti-inflammatory, and antioxidant. But, the poor water solubility of yeast β-glucan limits its application. In this study, some carboxymethylated yeast β-glucans (CMGs) with different substitution degree was prepared.

Extended One-Way Waveguide States of Large-Area Propagation in Gyromagnetic Photonic Crystals.

We propose extended one-way waveguide states of large-area propagation in a photonic crystal waveguide consisting of two honeycomb gyromagnetic photonic crystals with opposite external magnetic fields. When the width of the waveguide is small enough, the edge states along both boundaries of the waveguide couple with each other strongly and thus create the so-called extended one-way waveguide states. Of note, this structure supports both even and odd extended states, which can be excited under different excitation conditions.

Multifrequency electrical impedance tomography system based on undersampling combined with a fast digital demodulation algorithm.

Multifrequency electrical impedance tomography (MFEIT) has shown great application prospects in the field of biomedical imaging. To realize high-precision multifrequency electrical impedance information acquisition, a high-precision MFEIT system with undersampling combined with a fast digital demodulation algorithm is proposed. The system is integrated with 16 electrodes, and semi-parallel acquisition is used.

A Mechanistic Approach on Perception Mode of ABA Receptors (PYLs) to Novel Opabactin Analogues.

This study explored the structural mechanisms governing the binding of opabactin (OP) analogues - to abscisic acid (ABA) receptors by employing a combination of micro-scale thermophoresis (MST), phosphatase activity inhibition assays, and molecular dynamics simulations. The compounds - selectively activated PYR1, PYL2, and PYL6, while exhibiting minimal activity against PYL10, thus identifying them as selective ABA receptor agonists. Additionally, these analogues exerted a significant inhibitory effect on the phosphatase HAB1 upon binding to the receptors.

oriTDB: a database of the origin-of-transfer regions of bacterial mobile genetic elements.

Conjugation and mobilization are two important pathways of horizontal transfer of bacterial mobile genetic elements (MGEs). The origin-of-transfer (oriT) region is crucial for this process, serving as a recognition site for relaxase and containing the DNA nicking site (nic site), which initiates the conjugation or mobilization. Here, we present a database of the origin-of-transfer regions of bacterial MGEs, oriTDB (https://bioinfo-mml.

Effect of Exogenous Melatonin on Performance and Mastitis in Dairy Cows.

Mastitis is an important factor affecting the health of cows that leads to elevated somatic cell counts in milk, which can seriously affect milk quality and result in huge economic losses for the livestock industry. Therefore, the aim of this trial was to investigate the effect of melatonin on performance and mastitis in dairy cows. Forty-eight Holstein cows with a similar body weight (470 ± 10 kg), parity (2.

Altechromone A Ameliorates Inflammatory Bowel Disease by Inhibiting NF-κB and NLRP3 Pathways.

Altechromone A, also known as 2,5-dimethyl-7-hydroxychromone, is a hydroxyketone containing one hydroxyl and one ketone group. In this study, we isolated Altechromone A from the marine-derived fungus (XY-14-0-4). Previous reports show that Altechromone A has various activities including tumor suppression, antibacterial, and antiviral activities.

and verification of the mechanism of formononetin in treating hepatocellular carcinoma.

Background: Hepatocellular carcinoma (HCC) remains a significant global medical challenge. Formononetin, an isoflavone derived from , has been shown to have various regulatory effects on HCC. However, the exact molecular mechanism by which formononetin acts against HCC is still unclear.

Metabolomics-based profiling of anti-inflammatory compounds from in shanghe, China.

is a popular herb used in foods, cosmetics, and medicines. In the present study, liquid chromatography-mass spectrometry-based metabolomics analysis and the zebrafish model were used to investigate the potential biomarkers of growing in Shanghe County (Shandong Province, China) and their anti-inflammatory properties. Network pharmacology and molecular docking were performed to screen the main targets of the characteristic compounds to understand their mechanisms of action.

Exploring serum -glycome patterns as candidate non-invasive biomarkers in inguinal hernia.

Introduction: Although inguinal hernia (IH) is prevalent in elderly males, research on its specific diagnostic biomarkers is limited. Protein -glycosylation is one of the most important and ubiquitous post-translational modifications and often results in a remarkable heterogeneity of protein glycoforms. Protein -glycosylation often changes in a disease and holds great potential for discovering non-invasive biomarkers.

The Effects of -citrulline Supplementation on the Athletic Performance, Physiological and Biochemical Parameters, Antioxidant Capacity, and Blood Amino Acid and Polyamine Levels in Speed-Racing Horses.

The objective of this study was to evaluate the effects of pre-exercise -citrulline supplementation on the athletic performance of speed-racing horses during a high-intensity exercise. On the 20th day of the experiment, blood samples were collected at 3 h and 6 h post-supplementation to measure the amino acid and polyamine concentrations. On the 38th day of the experiment, the horses participated in a 2000 m speed race, and three distinct blood samples were gathered for assessing blood gases, hematological parameters, the plasma biochemistry, antioxidant parameters, and NO concentrations.

Efficacy and safety of second-line therapies for advanced hepatocellular carcinoma: a network meta-analysis of randomized controlled trials.

Background: The selection of appropriate second-line therapy for liver cancer after first-line treatment failure poses a significant clinical challenge due to the lack of direct comparative studies and standard treatment protocols. A network meta-analysis (NMA) provides a robust method to systematically evaluate the clinical outcomes and adverse effects of various second-line treatments for hepatocellular carcinoma (HCC).

Methods: We systematically searched PubMed, Embase, Web of Science and the Cochrane Library to identify phase III/IV randomized controlled trials (RCTs) published up to March 11, 2024.

Dual-Energy Computed Tomography Iodine Maps: Application in the Diagnosis of Periprosthetic Joint Infection in Total Hip Arthroplasty.

Background: The challenge of early and rapid diagnosis of periprosthetic joint infection (PJI) remains important. This study aimed to assess the efficacy of dual-energy computed tomography (DECT) iodine maps for diagnosing PJI in total hip arthroplasty.

Methods: We prospectively enrolled 68 patients who had postoperative joint pain after hip arthroplasty.

Whole genome sequencing insight into carbapenem-resistant and multidrug-resistant harboring chromosome-borne .

Carbapenem-resistant (CRAB) poses a significant threat to hospitalized patients as effective therapeutic options are scarce. Based on the genomic characteristics of the CRAB strain AB2877 harboring chromosome-borne , which was isolated from the bronchoalveolar lavage fluid (BALF) of a patient in a respiratory intensive care unit (RICU), we systematically analyzed antibiotic resistance genes (ARGs) and the genetic context associated with ARGs carried by CRAB strains harboring chromosome-borne worldwide. Besides , other ARGs were detected on the chromosome of the CRAB strain AB2877 belonging to ST208/1806 (Oxford MLST scheme).

[Determination of sodium cyclamate in Baijiu by pre-column derivatization-high performance liquid chromatography with fluorometric detection].

Sodium cyclamate in Baijiu is a key item in the China National Food Safety Supervision and Inspection Plan. A simple, economical, sensitive, and reliable method is urgently needed for routine analysis and internal quality control. A method based on high performance liquid chromatography with fluorescence detection (HPLC-FLD) was developed for the determination of sodium cyclamate in Baijiu by -phthalaldehyde derivatization.

Effects of lysine and threonine on milk yield, amino acid metabolism, and fecal microbiota of Yili lactating mares.

The nutritional benefits of mare milk are attracting increasing consumer interest. Limited availability due to low yield poses a challenge for widespread adoption. Although lysine and threonine are often used to enhance protein synthesis and muscle mass in horses, their impact on mare milk yield and nutrient composition remains underexplored.