First Report of Rot Disease Caused by in China.

Rosa roxburghii Tratt is a rosaceous shrub originating from southwest China (Fu et al. 2020). From September to October 2022, R.

Protocol to identify functional doppelgängers and verify biomedical gene expression data using doppelgangerIdentifier.

Functional doppelgängers (FDs) are independently derived sample pairs that confound machine learning model (ML) performance when assorted across training and validation sets. Here, we detail the use of doppelgangerIdentifier (DI), providing software installation, data preparation, doppelgänger identification, and functional testing steps. We demonstrate examples with biomedical gene expression data.

Doppelgänger spotting in biomedical gene expression data.

Doppelgänger effects (DEs) occur when samples exhibit chance similarities such that, when split across training and validation sets, inflates the trained machine learning (ML) model performance. This inflationary effect causes misleading confidence on the deployability of the model. Thus, so far, there are no tools for doppelgänger identification or standard practices to manage their confounding implications.

How doppelgänger effects in biomedical data confound machine learning.

Machine learning (ML) models have been increasingly adopted in drug development for faster identification of potential targets. Cross-validation techniques are commonly used to evaluate these models. However, the reliability of such validation methods can be affected by the presence of data doppelgängers.

The CDK inhibitor CR8 acts as a molecular glue degrader that depletes cyclin K.

Molecular glue compounds induce protein-protein interactions that, in the context of a ubiquitin ligase, lead to protein degradation. Unlike traditional enzyme inhibitors, these molecular glue degraders act substoichiometrically to catalyse the rapid depletion of previously inaccessible targets. They are clinically effective and highly sought-after, but have thus far only been discovered serendipitously.

Highly extended filaments in aqueous gold nano-particle colloidals.

A new regime of filamentation has been discovered in aqueous gold nanoparticle colloidals (AGNC). Different from filamentation in liquids, in this regime, by doping water with gold nanoparticles, there is no observable multiple small-scale filaments, but instead a spatially continuous plasma channel is formed. The length of the filament is more than ten times as compared with that in water.

Identification and characterization of microRNAs and their target genes from Nile tilapia (Oreochromis niloticus).

MicroRNAs (miRNAs) are a class of small single-stranded, endogenous 21-22 nt non-coding RNAs that regulate their target mRNA levels by causing either inactivation or degradation of the mRNAs. In recent years, miRNA genes have been identified from mammals, insects, worms, plants, and viruses. In this research, bioinformatics approaches were used to predict potential miRNAs and their targets in Nile tilapia from the expressed sequence tag (EST) and genomic survey sequence (GSS) database, respectively, based on the conservation of miRNAs in many animal species.

Detection of three common, deletional alpha-thalassemia determinants in Southern China by a single-tube multiplex polymerase chain reaction method.

alpha-Thalassemia (thal) is one of the most common inherited disorders in the world and in Southern China. Three large deletions of the alpha-globin gene, namely the -alpha3.7 and -alpha4.

Expression of a novel HsMCAK mRNA splice variant, tsMCAK gene, in human testis.

Identification of specifically expressed genes in the adult or fetal testis is very important for the study of genes related to the development and function of the testis. In this study, a human adult testis cDNA microarray was constructed and hybridized with 33P-labeled human adult and embryo testis cDNA probes, respectively. After differential display analyzing, a number of new genes related to the development of testis and spermatogenesis had been identified.

Calibration error on the measurement of back vertex power for contact lenses with method using focimeter with manual focusing.

Purpose: The International Standard ISO 9337-1, which sets forth the method for measuring back vertex power of contact lenses with manually focusing focimeters, specifies that test lenses conforming to ISO 9342 may be used to calibrate focimeters on its spectacle lens support, and correction values obtained in this way can be used directly in measuring contact lenses on its contact lens support. This study was conducted because of concern that the method mentioned in ISO 9337-1 is not good enough for the calibration of focimeters used to measure contact lenses.

Methods: To test the validity of this method, a research group from China National Institute of Metrology (NIM) studied it theoretically and carried out a series of comparison experiments, respectively, with the conventional test lenses conforming to ISO 9342 and test lenses made at NIM (with an expanded uncertainty of 0.