Publications by authors named "Li Ningxing"

Article Synopsis
  • Extracellular proteases, particularly chitinases from the fungus Arthrobotrys oligospora, aid in nematode infection, yet how microRNAs regulate gene expression in this context is not well understood.
  • Transcriptome sequencing was performed to identify and analyze chitin-responsive microRNAs (miRNAs), revealing that 25 out of 85 novel miRNAs significantly changed expression in response to chitin.
  • Target gene analysis indicated that these differentially expressed miRNAs (DEmiRNAs) are linked to biological processes like biodegradation and cell cycle regulation, while specific interactions, such as the chitinase AOL_s00004g379 being a target of miR_70, were confirmed through dual-luciferase assays.
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Fascioliasis, a global zoonotic parasitic disease, is mainly caused by Fasciola hepatica (F. hepatica) parasitizing in the livers of hosts, mainly humans and herbivores. Glutathione S-transferase (GST) is one of the important excretory- secretory products (ESPs) from F.

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Chitinase AO-801 is a hydrolase secreted by Arthrobotrys oligospora during nematode feeding, while its role remained elusive. This study analyzed the molecular characteristics of recombinant chitinase of Arthrobotrys oligospora (reAO-801). AO-801 belongs to the typical glycoside hydrolase 18 family with conserved chitinase sequence and tertiary structure of (α/β)8 triose-phosphate isomerase (TIM) barrel.

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In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite. This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3', 5, 5'-tetramethylbenzidine (TMB). The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed.

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Article Synopsis
  • An ultrasensitive sensor for detecting mercury ions (Hg2+) was developed using a novel isothermal cycling reaction combined with dsDNA-templated copper nanoparticles (CuNPs).
  • The sensor features a unique hairpin DNA that, upon interaction with Hg2+, modifies its structure to become susceptible to digestion by Exo III enzymes, enabling signal amplification.
  • The method allows for detecting Hg2+ concentrations over a wide range (5 orders of magnitude) with a low detection limit of 3.9 pM and demonstrates high selectivity and effective real-water sample recovery results.
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The viral capsid protein p24 of human immunodeficiency virus is expressed at different level during viral invasion. Detection of p24 is of great importance in acquired immunodeficiency syndrome monitoring and therapy. A ratiometric probe that is easily-synthesized was constructed based on self-assembled fluorescent Ce(Ⅲ) and fluorescein.

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In this work, a kind of environment-friendly and water-dispersible silicon nanodot (SiND) was rapidly synthesized by using the mild reagents (3-aminopropyl)triethoxysilane (APTES) and glucose. It was found that the fluorescence of the as-prepared SiNDs can be quenched obviously by permanganate due to the inner filter effect. Inspired by this finding, a novel fluorescent sensor for sensitive detection of hydrogen peroxide (HO) was developed through the oxidation-reduction reaction between permanganate and HO.

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In this work, a three-dimensional DNA machine based on the isothermal strand-displacement polymerase reaction (ISDPR) has been constructed. The walking behavior of a DNA walker on the obstructive surface of magnetic beads has also been studied by adding different nucleic acid blocks. The "leg" of the DNA walker could hybridize with a hairpin structure DNA named H1 and lead to the opening of it.

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In this study, two kinds of sensitive biosensors based on a multipedal DNA walker along a three-dimensional DNA functional magnet particles track for the chemiluminescent detection of streptavidin (SA) are constructed and compared. In the presence of SA, a multipedal DNA walker was constructed by a biotin-modified catalyst as a result of the terminal protection to avoid being digested by exonuclease I. Then, through a toehold-mediated strand exchange, a "leg" of a multipedal DNA walker interacted with a toehold of a catalyzed hairpin assembly (CHA)-H1 coupled with magnetic microparticles (MMPs) and opened its hairpin structure.

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Synthetic enzyme-free DNA nanomachine performs quasi-mechanical movements in response to external intervention, suggesting the promise of constructing sensitive and specific biosensors. Herein, a smart DNA nanomachine biosensor for biomolecule (such as nucleic acid, thrombin and adenosine) detection is developed by target-assisted enzyme-free hairpin DNA cascade amplifier. The whole DNA nanomachine system is constructed on gold nanoparticle which decorated with hundreds of locked hairpin substrate strands serving as DNA tracks, and the DNA nanomachine could be activated by target molecule toehold-mediated exchange on gold nanoparticle surface, resulted in the fluorescence recovery of fluorophore.

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An enzyme-free stochastic DNA walker propelled by a single catalytic or double catalytic DNA assembly has been constructed. The application of the proposed DNA walking biosensor was successfully expanded to the detection of DNA and the enzymatic activity of T4 polynucleotide kinase.

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An ultrasensitive chemiluminescence (CL) biosensor for the detection of protein is developed in this study based on the functionalized magnetic microparticles (MMPs) and the hybridization chain reaction (HCR). First, the primer hybridized with the thrombin aptamer conjugated on the surface of MMPs. Then the HCR was triggered by part of the primer and its products were assembled on the surface of the MMPs.

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Fluorescent magnetic multifunctional microparticles were fabricated by a facile droplet microfluidic strategy. Two sodium alginate streams, one doped with Fe3O4 nanoparticles (NPs) and the other with CdSe/ZnS quantum dots, were introduced into a flow-focusing channel as a type of parallel laminar flow to form droplets containing two distinct parts. Then, at the serpentine channel, the Ca(2+) in the oil phase diffused into the droplets, causing the solidification of the droplets.

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A target-catalyzed hairpin assembly (CHA) and graphene/Au-NPs hybrids-based platform has been developed for the determination of DNA. This new sensor not only avoided any labeling but also reduced the background signal. In the absence of target, the assembly of H1 and H2 couldn't be triggered.

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An ultrasensitive method for the detection of protein is critically important in fundamental research and practical applications due to the low abundance of disease markers in body fluids or tissues. To detect the trace levels of disease markers with high sensitivity and specificity, a sensitive colorimetric biosensor for protein assay was developed using gold nanoparticles (AuNPs) and rolling circle amplification (RCA). After binding the biotinylated primer/circular template to the streptavidin-conjugated sandwich ELISA immunocomplex, the biotinylated primer was isothermally extended to generate single-stranded DNA (ssDNA).

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A universal, highly sensitive and selective chemiluminescence (CL) imaging method has been developed for high throughput detection of DNA. After molecular beacon (MB) hybridized with the target DNA, the biotin-labeled primer was attached to a magnetic microparticle (MMP) surface by hybridization with the stem part of the MB to initiate a polymerization of DNA strand, which led to the release of the target and another polymerization cycle. Thus the polymerization produced the multiplication of biotin-labeled primer on the surface of MMPs.

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