Intracoronary infusion of Wharton's jelly-derived mesenchymal stem cells in acute myocardial infarction: double-blind, randomized controlled trial.

Background: The use of adult stem cells is limited by the quality and quantity of host stem cells. It has been demonstrated that Wharton's jelly-derived mesenchymal stem cells (WJMSCs), a primitive stromal population, could integrate into ischemic cardiac tissues and significantly improve heart function. In this randomized, controlled trial, our aim was to assess the safety and efficacy of intracoronary WJMSCs in patients with ST-elevation acute myocardial infarction (AMI).

Molecular design of new aggrecanases-2 inhibitors.

Aggrecanases-2 is a very important potential drug target for the treatment of osteoarthritis. In this study, a series of known aggrecanases-2 inhibitors was analyzed by the technologies of three-dimensional quantitative structure-activity relationships (3D-QSAR) and molecular docking. Two 3D-QSAR models, which based on comparative molecular field analysis (CoMFA) and comparative molecular similarity analysis (CoMSIA) methods, were established.

CD106 identifies a subpopulation of mesenchymal stem cells with unique immunomodulatory properties.

Mesenchymal stem cells (MSCs) reside in almost all of the body tissues, where they undergo self-renewal and multi-lineage differentiation. MSCs derived from different tissues share many similarities but also show some differences in term of biological properties. We aim to search for significant differences among various sources of MSCs and to explore their implications in physiopathology and clinical translation.

Molecular design of anticancer drug leads based on three-dimensional quantitative structure-activity relationship.

Heat shock protein 90 (Hsp90) takes part in the developments of several cancers. Novobiocin, a typically C-terminal inhibitor for Hsp90, will probably used as an important anticancer drug in the future. In this work, we explored the valuable information and designed new novobiocin derivatives based on a three-dimensional quantitative structure-activity relationship (3D QSAR).

Second chemiluminescence behavior of Fe2+, Fe3+ and Cr3+ in the luminal-KMnO4 system.

A novel flow injection chemiluminescence (CL) method has been developed for the determination of three metal ions, namely Fe2+, Fe3+ and Cr3+, based on the second CL (SCL) signal of the mixture of luminal with KMnO4 in a sodium hydroxide medium by the catalysis of Fe2+, Fe3+ or Cr3+. The possible CL mechanism of the systems, the influencing factors, and the optimum conditions for the reactions were investigated based upon the kinetic curve of the CL reaction, CL spectra, UV-visible spectra and some other experiments. Under the optimum conditions, the SCL intensity was directly proportional to the concentration of these metal ions in solution in the range of 0.

A new chemiluminescence method for the determination of nickel ion.

A new chemiluminescence (CL) phenomenon described as the second-chemiluminescence (SCL) was observed and a strong CL signal was detected, when Ni(II) ion was injected into the mixture after the end of the reaction of potassium permanganate with alkaline luminol. The possible CL mechanism is proposed based on the kinetic curve of the CL reaction, CL spectra, UV-vis spectra and some other experiments. A flow-injection analysis for the determination of nickle(II) ion has been developed, based on the catalysis of nickel(II) ion on the CL reaction between potassium manganate produced on-line and luminol under alkaline condition.

Permanganate-based chemiluminescence analysis of ferulic acid using flow injection.

A simple, sensitive and rapid flow-injection chemiluminescence method has been developed for the determination of ferulic acid based on the chemiluminescence reaction of ferulic acid with potassium permanganate in a nitric acid medium. A strong chemiluminescence signal was observed when ferulic acid was injected into an acidic potassium permanganate solution in a flow-cell. The present method allowed the determination of ferulic acid in the concentration range of 6.

Novel SLA class I alleles of Chinese pig strains and their significance in xenotransplantation.

To lay background for studying rejection mechanisms in xenotransplantation and developing the strategies for intervention, class I genes of swine leukocyte antigens (SLA) of three Chinese pig strains Bm, Gz and Yn were cloned and sequenced. The cDNA of the class I loci P1 and P14 were amplified by RT-PCR and subjected to insert into sequencing vectors. All six allelic sequences we examined, each two for one Chinese strain, are not identical to those reported, which allows these novel sequences receiving their accession numbers AY102467-AY102472 from GenBank.