Circadian signatures of adipose tissue in diet-induced obesity.

High-fat diet (HFD) feeding rewires circadian rhythms of peripheral organs including the liver and adipose tissue. While the liver has been extensively studied, it remains largely unknown whether and how HFD organizes circadian biology in adipose tissue. Here, we took a systems approach to profile the diurnal transcriptome of adipose tissue in diet-induced obese mice either fed a low-fat diet (LFD) that reduces weight or still fed HFD.

Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice.

Comparative gene identification-58 (CGI-58), also known as α/β hydrolase domain containing 5, is the co-activator of adipose triglyceride lipase that hydrolyzes triglycerides stored in the cytosolic lipid droplets. Mutations in CGI-58 gene cause Chanarin-Dorfman syndrome (CDS), an autosomal recessive neutral lipid storage disease with ichthyosis. The liver pathology of CDS manifests as steatosis and steatohepatitis, which currently has no effective treatments.

The predictive factors of successful sperm retrieval for men with Y chromosome AZFc microdeletion.

Purpose: To identify key predictors for successful sperm retrieval in men with AZFc microdeletion.

Methods: Totally, 71 infertile men with confirmed AZFc microdeletion were studied. For each patient, the endocrine profile including serum follicle stimulating hormone (FSH), luteinizing hormone, total testosterone, prolactin, and estradiol was recorded, along with intratesticular testosterone levels (ITT), age, and testicular size.

Identification of the novel natural product inhibitors of SHP2 from the plant Toona sinensis: In vitro and in silico study.

In this study, we tested the inhibitory activity of 45 natural products extracted from the plant Toona sinensis on SHP2 protein, and identified four natural product inhibitors. The natural product 1,2,3,6-Tetragalloylglucose (A-1) was first reported as a competitive inhibitor of SHP2, with an IC value of 0.20 ± 0.

[Potentilla anserina polysaccharide alleviates the damage of mouse glomerular mesangial cells induced by high glucose via promoting the expression of circ-AKT3].

Objective To explore the effect of Potentilla anserina polysaccharide on the injury of glomerular mesangial cells induced by high glucose and its possible mechanism. Methods High glucose was used to induce SV40 MES 13 mouse glomerular mesangial cells to establish a cell injury model, and 0.1, 0.

Semantic-Powered Explainable Model-Free Few-Shot Learning Scheme of Diagnosing COVID-19 on Chest X-Ray.

Chest X-ray (CXR) is commonly performed as an initial investigation in COVID-19, whose fast and accurate diagnosis is critical. Recently, deep learning has a great potential in detecting people who are suspected to be infected with COVID-19. However, deep learning resulting with black-box models, which often breaks down when forced to make predictions about data for which limited supervised information is available and lack inter-pretability, still is a major barrier for clinical integration.

A hybrid hemodynamic knowledge-powered and feature reconstruction-guided scheme for breast cancer segmentation based on DCE-MRI.

Automatically and accurately annotating tumor in dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), which provides a noninvasive in vivo method to evaluate tumor vasculature architectures based on contrast accumulation and washout, is a crucial step in computer-aided breast cancer diagnosis and treatment. However, it remains challenging due to the varying sizes, shapes, appearances and densities of tumors caused by the high heterogeneity of breast cancer, and the high dimensionality and ill-posed artifacts of DCE-MRI. In this paper, we propose a hybrid hemodynamic knowledge-powered and feature reconstruction-guided scheme that integrates pharmacokinetics prior and feature refinement to generate sufficiently adequate features in DCE-MRI for breast cancer segmentation.

Ferroportin-dependent ferroptosis induced by ellagic acid retards liver fibrosis by impairing the SNARE complexes formation.

Chronic liver injury causing liver fibrosis is a major cause of morbidity and mortality worldwide. Targeting the suppression of hepatic stellate cell (HSC) activation is recognized as an effective strategy for the treatment of liver fibrosis. Ellagic acid (EA), a natural polyphenol product isolated from fruits and vegetables, possesses many biological functions.

Feature Selection Model Based on IWOA for Behavior Identification of Chicken.

In order to reduce the influence of redundant features on the performance of the model in the process of accelerometer behavior recognition, and to improve the recognition accuracy of the model, this paper proposes an improved Whale Optimization algorithm with mixed strategy (IWOA) combined with the extreme gradient boosting algorithm (XGBoost) as a preferred method for chicken behavior identification features. A nine-axis inertial sensor was used to obtain the chicken behavior data. After noise reduction, the sliding window was used to extract 44 dimensional features in the time domain and frequency domain.

Role of Collagen in Vascular Calcification.

Vascular calcification is a pathological process characterized by ectopic calcification of the vascular wall. Medial calcifications are most often associated with kidney disease, diabetes, hypertension, and advanced age. Intimal calcifications are associated with atherosclerosis.

Association Between Hospice Enrollment and Total Health Care Costs for Insurers and Families, 2002-2018.

Importance: Use of hospice has been demonstrated to be cost saving to the Medicare program and yet the extent to which hospice saves money across all payers, including whether it shifts costs to families, is unknown.

Objective: To estimate the association between hospice use and total health care costs including family out-of-pocket health care spending.

Design Setting And Participants: This retrospective cohort study of health care spending in the last 6 months of life used data from the nationally representative Medicare Current Beneficiary Survey (MCBS) between the years 2002 and 2018.

Nε-Carboxymethyl-Lysine Mediates Vascular Calcification in Diabetes Caused by Impaired Osteoclastic Resorption Activity Through NFATc1-GNPTAB.

Nε-carboxymethyl-lysine (CML) is closely associated with vascular calcification in diabetes. Osteoclasts are the only cells with bone resorption activity that have the potential to reverse calcification. This study aimed to investigate the mechanism of CML in the bone resorption activity of macrophage-derived osteoclasts in diabetic calcified plaques.

Selection and characterization of plant-derived alkaloids with strong antialgal inhibition: growth inhibition selectivity and inhibitory mechanism.

In recent years, researches on microalgae inhibition with plant-derived active substances have attracted much attention. In this study, the inhibition of six plant-derived alkaloids (neferine, isoliensinine, linensinine, nuciferine, capsaicin, and hordenine) on bloom-forming cyanobacteria Microcystis aeruginosa were investigated. The results showed that neferine and nuciferine had stronger inhibition on the growth of M.

Role of endothelial cells in vascular calcification.

Vascular calcification (VC) is active and regulates extraosseous ossification progress, which is an independent predictor of cardiovascular disease (CVD) morbidity and mortality. Endothelial cells (ECs) line the innermost layer of blood vessels and directly respond to changes in flow shear stress and blood composition. Together with vascular smooth muscle cells, ECs maintain vascular homeostasis.

A Sensitive Fluorescent Immunoassay for Prostate Specific Antigen Detection Based on Signal Amplify Strategy of Horseradish Peroxidase and Silicon Dioxide Nanospheres.

A simple, sensitive, and fluorescent immunoassay for the detection of prostate-specific antigen (PSA) based on horseradish peroxidase and silicon dioxide nanospheres as a signal amplification strategy has been described. In the design, the primary antibody (Ab) of PSA was first immobilized on the 96-well plates via physical adsorption between polystyrene and hydrophobic groups of the antibody molecule. The silicon dioxide nanospheres (SiO NSs), with large surface area and good biocompatibility, were loaded with horseradish peroxidase (HRP) and horseradish peroxidase-labeled secondary antibodies (HRP-Ab) as signal amplification systems.

Simple high-temperature annealing affords commercial carbon cloth with enhanced electrochemical performance for highly sensitive detection of imidacloprid.

Imidacloprid (IDP) residue in modern agricultural production seriously endangers human health and environmental safety. The establishment of a rapid and efficient method for the detection of IDP residue can effectively prevent its harm to human health. Herein, we demonstrate the carbon cloth (CC) prepared by a high-temperature annealing strategy possesses enhanced electrochemical performance, which could be directly used in electrochemical IDP sensing.

Tissue engineered bone via templated hBMSCs mineralization and its application for bone repairing.

To develop bone implants, a novel tissue-engineered bone was constructed via templated human bone mesenchymal stem cells (hBMSCs) mineralization. Firstly, an osteoid-like template (Os-template) with aligned collagen fibers was prepared and followed by seeding hBMSCs to mimic the process of bone formation. After being cultured over weeks, the cells produced collagen fibers in an orderly aligned osteomorphic fashion.

Amine-Modified Chitosan Flocculant Synthesized via Single-Mode Microwave Method for Laundry Wastewater Treatment.

In this study, an effective and environmentally friendly polyaminated cross-linked chitosan (M-PACTS) flocculant was successfully synthesized via circular focus single-mode microwave synthesizer irradiation. Epichlorohydrin and tetraethylenepentamine were used as the cross-linking agent and active cationic reagent, respectively. The same formation was used to prepare cationic lightly cross-linked chitosan (C-PACTS) via the conventional heating method.

Amphiphilic nano-delivery system based on modified-chitosan and ovalbumin: Delivery and stability in simulated digestion.

Nano-delivery systems play an important role in the development of nutritional supplements due to their efficient encapsulation and delivery properties for nutrients. Herein, we prepared protein-polysaccharide nanoparticles as a novel amphiphilic nano-delivery system based on gallic acid modified chitosan (GCS) and ovalbumin (OVA) by pH-driven and calcium ion crosslinking. The nanoparticles loaded with hydrophilic riboflavin (Rib) and hydrophobic quercetin (Que) as nutrient models were abbreviated as GCS-OVA-Rib NPs and GCS-OVA-Que NPs, respectively.

Draft Genome Sequence of Stenotrophomonas maltophilia KJ, a Clinical Isolate from Taiwan.

We report the draft genome sequence of Stenotrophomonas maltophilia strain KJ, which was isolated from a sputum sample from a patient with a respiratory tract infection. Multilocus sequence typing analysis suggested that strain KJ belongs to a novel S. maltophilia sequence type.

The structural changes of gray matter in Parkinson disease patients with mild cognitive impairments.

Objectives: Parkinson disease (PD) is associated with cognitive impairments. However, the underlying neural mechanism of cognitive impairments in PD is still not clear. This study aimed to investigate the anatomic alternations of gray matter in PD patients with mild cognitive impairment (MCI) and their associations with neurocognitive measurements.

Cyclocytidine hydrochloride inhibits the synthesis of relaxed circular DNA of hepatitis B virus.

Background: Cyclocytidine hydrochloride (HCl) has been reported to inhibit DNA synthesis by affecting DNA polymerase. Here, we tested the antiviral effect of cyclocytidine on hepatitis B virus (HBV) DNA synthesis, which is reliant on DNA polymerase activity.

Materials And Methods: Cyclocytidine HCl was treated to HBV-producing HepAD38 cells or added to an endogenous polymerase reaction, and HBV DNA was detected by Southern blot.