Preparation and dynamic color-changing study of fluorescent polymer nanoparticles for individualized and customized anti-counterfeiting application.

Preparing new fluorescent materials for individualized and customized anti-counterfeiting applications to meet needs from the rapid development of e-commerce is of great significance. This paper reports the preparation of dynamic color-changing fluorescent polymer nanoparticles (PNPs) by constructing a fluorescence resonance energy transfer (FRET) pair between aggregation-induced emission (AIE) structures and photochromic structures. At first, methyl methacrylate (MMA) was used as the main monomer and tetraphenylethylene (TPE, a typical AIE structure) modified methacrylate (TPE-MA) and photochromic spiropyran (SP) modified methacrylate (SP-MA) as minor monomers were copolymerized to obtain the ternary copolymer PMMA-TPE-SP.

Conjugated Polyelectrolyte Containing a High Density of Pendant Phenylboronic Acid Groups for Dopamine Detection.

A fluorescent sensing system based on a conjugated polyelectrolyte was constructed to detect dopamine (DA) in complex samples. The conjugated polymer PFPE-PBA with poly[fluorenyl---phenyleneethynylene] (PFPE) as the backbone and carrying four pendant phenylboronic acid (PBA) groups in each repeat unit was synthesized. PFPE-PBA was found to have good solubility in polar solvents.

Strongly fluorescent conjugated polymer nanoparticles in aqueous colloidal solution for universal, efficient and effective development of sebaceous and blood fingerprints.

Taking the same developing strategy for different types of latent fingerprints is helpful in improving the efficiency of criminal investigation. Here we advanced a new strategy based on amino-functionalized poly(p-phenylenevinylene) nanoparticles (PPV-brPEI NPs) in aqueous colloidal solution as the developing reagent. The desirable amino functionality and strong emission of NPs were simultaneously realized by adding branched polyethyleneimine (brPEI) during the process of thermal elimination of the PPV polymer precursor.

Dynamic Fluorescent Anti-Counterfeiting Labels Based on Conjugated Polymers Confined in Submicron Fibrous Membranes.

Developing a new anti-counterfeiting strategy is of great significance to combating the global counterfeiting problem. Here we report the construction of a dynamic fluorescence response system for anti-counterfeiting by combining the photochromism induced by the ring-opening of spiropyran (SP) to merocyanine (MC) with the fluorescence resonance energy transfer (FRET) between the conjugated polymer and MC. After elucidating the design principle, a new conjugated polymer, PPETE-SP, consisting of a poly[-(phenylene ethynylene)--(thienylene-ethynylene)] (PPETE) backbone with pendant SP, was synthesized and characterized.

['s clinical experience in treatment of tic disorder with acupuncture technique of cultivating the primary and regulating the mind].

The paper introduces the clinical experience of in treatment of tic disorder. believes that tic disorder results from the primary deficiency and mind disturbance. Acupuncture for cultivating the primary and regulating the mind is proposed specially for tic disorder.

Thalidomide Reduces Activation of Murine Pancreatic Stellate Cells by Inhibiting the TGF-β/Smad Pathway.

Objective: The aim of this study was to investigate the effects and mechanism of thalidomide on pancreatic stellate cell (PSC) activation in mice and to find the optimal timing of thalidomide administration.

Methods: PSCs, isolated from mouse pancreas tissue, were divided into five groups with specific treatments: (A) control PSCs (PSC), (B) PSCs induced by TGF-β1 (PSC+TGF-β1), (C) PSCs induced by TGF-β1 followed by thalidomide (PSC+TGF-β1+Thalidomide), (D) PSCs receiving TGF-β1 and thalidomide simultaneously (PSC+(TGF-β1+Thalidomide)), and (E) PSCs treated with thalidomide only (PSC+Thalidomide). We measured the effects of thalidomide on PSC activation by detecting the expression of α-SMA, collagen type I, and the TGF-β/Smad pathway through quantitative real-time PCR and Western blot analysis.

Fluorescent Cationic Conjugated Polymer-Based Adaptive Developing Strategy for Both Sebaceous and Blood Fingerprints.

Both latent sebaceous and blood fingerprints may provide valuable information for forensic investigation. To detect both types of fingerprints with no need to predistinguish them, a new adaptive developing strategy was proposed. A cationic conjugated polymer with poly[-(phenylene ethylene)--(thienylene ethynylene)] backbone (PPETE-NMe) was synthesized, which was dissolved in ,-dimethylformamide (DMF) to form the developing solution.

Highly Stable, Nondestructive, and Simple Visualization of Latent Blood Fingerprints Based on Covalent Bonding Between the Fluorescent Conjugated Polymer and Proteins in Blood.

Latent blood fingerprints (LBFPs) can provide critical information of foul play and help identify the suspects at violent crime scenes. The current methods for LBFP visualization are still not satisfactory because of the low sensitivity or complicated protocol. This study demonstrates a simple and effective LBFP visualization strategy by integrating a new amphiphilic fluorescent amino-functionalized conjugated polymer with the cotton-pad developing protocol.

STAT3/miR-15a-5p/CX3CL1 Loop Regulates Proliferation and Migration of Vascular Endothelial Cells in Atherosclerosis.

Endothelial cell proliferation disorder caused by vascular injury seems to be one of the causes of atherosclerosis, which is the pathological basis of coronary heart disease. The role of STAT3 in the regulation of microRNAs and endothelial dysfunction in atherosclerosis is unclear. STAT3 can be activated by cytokine IL-6 and up regulate the expression of CX3CL1.

Supramolecular and Physically Double-Cross-Linked Network Strategy toward Strong and Tough Elastic Fibers.

The strength and toughness are two trade-off properties of a material, yet can achieve strong and tough materials by introducing sacrificial bonds into a system. Here, we present a four-component multiblock copolymer (mBCP) approach toward strong and tough elastic fibers, by introducing terpyridine moieties into poly(ether ester) mBCP elastomers. After coordination with Fe(II), supramolecular cross-links are formed within the physically cross-linked thermoplastic elastomers.

Safflor yellow A protects vascular endothelial cells from ox-LDL-mediated damage.

Atherosclerosis is a chronic disease of arteries, which constitutes the pathological basis of a series of cardiovascular diseases. The inflammatory response of vascular endothelial cells mediated by oxidized low density lipoprotein (ox-LDL) is the early behavior and main signal of atherosclerosis. In this study, the damage model of vascular endothelial cells treated with ox-LDL was used to reproduce the damage process of vascular endothelial cells in the process of atherosclerosis.

Preparation of Fluorescence-Encoded Microspheres Based on Hydrophobic Conjugated Polymer-Dye Combination and the Immunoassay.

Fluorescent microspheres are greatly demanded in many applications based on high-throughput suspension array technology. To realize the multiplexed assay, microspheres should be encoded to identify the interaction between analytes and spheres. This study advanced a strategy for preparing fluorescence-encoded microspheres, employing two hydrophobic fluorophores, poly(-phenyleneethylene) (PPE), and Nile Red (NR), as well as the monodisperse amino-modified porous substrate polymeric spheres, poly(glycidyl methacrylate) microspheres (APGMA).

Monodisperse Bismuth-Halide Double Perovskite Nanocrystals Confined in Mesoporous Silica Templates.

Metal halide perovskites have fascinating electronic properties and have already been implemented in various devices. Although the behavior of the properties of lead halide perovskite nanocrystals has been studied, the properties of lead-free perovskite nanocrystals are less well-understood because synthesizing them is still very challenging. Here, a simple and popularizable method has been demonstrated to grow monodisperse bismuth-halide double perovskite nanocrystals, CsAgBiBr (1), inside three kinds of mesoporous silica templates.

The strong competitive role of 2n pollen in several polyploidy hybridizations in Rosa hybrida.

Background: 2n pollen play a strong competitive role in hybridization and breeding of multiploids in Rosa hybrida. The ploidy inheritable characteristic of 'Orange Fire' × 'Old Blush' were analyzed.

Result: The results of the cytological observations indicated that 2n pollen developed from the defeated cytoplasmic division or nuclear division in the meiosis metaphase II of PMC (pollen mother cell) in 'Old Blush'.

Preparation and evaluation of fluorescent poly(p-phenyleneethylene) covalently coated microspheres with reactive sites for bioconjugation.

Fluorescent microspheres with reactive sites for interacting with biomolecules are greatly demanded in flow cytometry based suspension array. Aiming to develop a new method for preparing fluorescent microspheres, two poly(p-phenyleneethylene) (PPE) conjugated polymers (CPs) with pedant carboxylic groups were synthesized via Sonogashira coupling and followed with hydrolysis of ester groups; then the conjugated polymers were immobilized onto monodispersed amino-modified porous poly(glycidylmethacrylate) (APGMA) microspheres via coupling reaction between carboxylic and amino groups to give APGMA-CP fluorescent microspheres. The fluorescent microspheres were found to have good photo- and thermal stability as well as negligible influence from rigorous washing.

Establishment of an efficient in vitro propagation system for Iris sanguinea.

Iris sanguinea is a perennial flowering plant that is typically cultivated through seeds or bulbs. However, due to limitations in conventional propagation, an alternate regeneration system using seeds was developed. The protocol included optimization of sterilization, stratification and scarification methods as iris seeds exhibit physiological dormancy.

MKL1/miR34a/FOXP3 axis regulates cell proliferation in gastric cancer.

Megakaryoblastic leukemia 1 (MKL1) was closely related to the pathogenesis of various human malignant cancers. MiR34a was reported to be closely related to cancer cell proliferation. Forkhead box protein 3 (FOXP3) was a transcription factor that played a different role in different cancer types.

PKM2 promotes glucose metabolism through a let-7a-5p/Stat3/hnRNP-A1 regulatory feedback loop in breast cancer cells.

Tumor cells metabolize more glucose to lactate in aerobic or hypoxic conditions than normal cells. Pyruvate kinase isoenzyme type M2 (PKM2) is crucial for tumor cell aerobic glycolysis. We established a role for let-7a-5p/Stat3/hnRNP-A1/PKM2 signaling in breast cancer cell glucose metabolism.

Long noncoding RNA H19 competitively binds miR-93-5p to regulate STAT3 expression in breast cancer.

The long noncoding RNA H19 is overexpressed in many cancers and acts as an oncogene. Here, we explored the role of H19 in breast cancer cells, including the effect of H19 on proliferation, migration, and invasion of breast cancer cells. We also investigated the relation of H19 to microRNA miR-93-5p and signal transducers and activators of transcription 3 (STAT3), the target gene of miR-93-5p.

Fluorescence development of fingerprints by combining conjugated polymer nanoparticles with cyanoacrylate fuming.

Selecting appropriate developing methods/reagents or their combination to enhance the effect for fingerprint development is of great significance for practical forensic investigation. Ethyl-2-cyanoacrylate ester (superglue) fuming is a popular method for "in-situ" developing fingerprints in forensic science, followed by fluorescence staining to enhance the contrast of the fingerprint image in some occasion. In this study, a series of fluorescent poly(p-phenylene vinylene) (PPV) nanoparticles (NPs) in colloidal solution were successfully prepared and the emission color was tuned via a simple way.

Detection of Sudan Dyes Based on Inner-Filter Effect with Reusable Conjugated Polymer Fibrous Membranes.

Developing effective methods for detecting illegal additives in food or seasoning is of great significance. In this study, a sensing strategy for selective detection of Sudan dyes was designed based on the fluorescence inner-filter effect (IFE) by using poly(phenylenevinylene) (PPV) solid materials in combination with an optimized experimental protocol. Two types of fluorescent solid materials, electrospun fibrous membranes and drop-cast films, were fabricated with PPV as the fluorophore and poly(vinyl alcohol) as the matrix, respectively.