Carbene-supported triphosphorus anion.

The stabilization of reactive anionic main group species utilizing carbenes constitutes a burgeoning and scarcely explored field. Herein, we report the synthesis of an anionic triphosphorus [P]- unit, supported by two cyclic (alkyl)(amino)carbenes (CAACs) in the form of potassium salts. This anion features a planar W-shaped conjugated C-P-P-P-C framework, characterized by 5-center-6-electron π delocalization and an additional σ lone pair located on each of the three phosphorus atoms.

Effectiveness, safety, and patterns of use of camrelizumab in advanced esophageal cancer: an individual patient data pooled analysis of 987 patients from three prospective cohort studies.

Background And Aims: This individual patient data pooled analysis aimed to evaluate the effectiveness, safety, and patterns of use of camrelizumab in a large cohort of advanced esophageal cancer (AEC) patients.

Approach And Results: Adult patients (≥ 18 years) who had received camrelizumab as part of AEC treatment were pooled from three independent, prospective observational cohort studies (NCT04616040, ChiCTR1900027275, and ChiCTR2000039499). The main outcomes were patterns of camrelizumab use, progression-free survival (PFS), overall survival (OS), and safety in the overall population and specific subgroups of underrepresented patients.

Radiation Dose-Volume Effects on Negative Tumor-Draining Lymph Nodes Impacted T-Cell Activation and Prognosis in Esophageal Cancer with Chemoradiotherapy.

Purpose: Preclinical model found that elective nodal irradiation (ENI) attenuated the efficacy of radiotherapy and radio-immunotherapy. However, limited clinical studies have explored the correlation between radiation dose-volume parameters of negative tumor draining lymph nodes (TDLNs) and T-cell activation/prognosis for cancer patients treated with definitive radio(chemo)therapy.

Experimental Design: Patients with locally advanced esophageal cancer undergoing definitive chemoradiotherapy (CRT) were selected from two prospective trials.

Serum Pharmacology Combining Network Pharmacology to Discover the Mechanism of Total Flavonoids in Aronia melanocarpa Fruit for Acute Lung Injury.

In this study, we used in vitro antioxidant assay and in vivo anti-inflammatory assay to comprehensively evaluate the activity of total flavonoids of Aronia melanocarpa fruit (AMF) in the treatment of acute lung injury (ALI) and explored the mechanism of action of total flavonoids of AMF in the treatment of ALI based on the method of serum medicinal chemistry combined with network pharmacology. In the present study, 107.37 mg g alcoholic extract of total flavonoids was used for the determination of in vitro antioxidant activity by UV spectrophotometric method; the ALI model mice were induced by lipopolysaccharide (LPS), and the organ index of mice, the wet-to-dry ratio of lungs, and the histopathological sections of lungs were measured; the levels of inflammatory factors (interleukin-1β [IL-1β], TL-6, and tumor necrosis factor-α [TNF-α]) were determined in serum and alveolar lavage fluid of mice by ELISA; the composition of AMF total flavonoids and the migratory components of blood were analyzed on the basis of UHPLC-MS/MS.

Clinical features and outcomes of unresectable locally advanced lung adenocarcinoma with uncommon mutations: a retrospective multi-center Chinese study.

Background: Uncommon epidermal growth factor receptor () gene mutant locally advanced non-small cell lung cancer (NSCLC) has been poorly documented in the literature. Our study aimed to investigate the clinical features and outcomes associated with these mutations.

Methods: A multi-center retrospective study was conducted to review 511 patients with mutant unresectable stage III lung adenocarcinoma, treated between 2012 and 2018 across 12 Chinese institutions.

Expression of interleukin-17 in oral tongue squamous cell carcinoma and its effect on biological behavior.

Tongue squamous cell carcinoma (TSCC) is a common malignant oral cancer characterized by substantial invasion, a high rate of lymph node and distant metastasis, and a high recurrence rate. This study aims to provide new ideas for the diagnosis and treatment of TSCC patients by exploring the related mechanisms that affect the migration and invasion of TSCC and inhibit the migration and spread of cancer cells. The results indicated the rate of high expression of IL-17 in cancer tissues was greater than that in tongue tissues, and the expression of IL-17 was related to the TNM stage.

[Posterior lateral perforator flap in lower limb combined with free fibula for maxillary tissue defect repair].

Objective: To investigate the effectiveness of posterior lateral perforator flap in lower limb combined with free fibula for maxillary tissue defect repair.

Methods: Between December 2018 and December 2023, 16 patients with the maxillary malignant tumors were admitted. There were 10 males and 6 females, with an average age of 64.

An efficient hydrogen evolution catalyst constructed using Pt-modified NiS/MoS with optimized kinetics across the full pH range.

Electrocatalyst materials play a crucial role in determining the efficiency of the hydrogen evolution reaction (HER), directly influencing the overall effectiveness of energy conversion technologies. NiS/MoS heterostructures hold substantial promise as bifunctional catalysts, owing to their synergistic electronic characteristics and plentiful active sites. However, their catalytic efficacy is impeded by the relatively elevated chemisorption energy of hydrogen-containing intermediates, which constrains their functionality in different pH environments.

Prospective multicenter study of camrelizumab in real-world settings for asian patients with esophageal squamous cell carcinoma.

Background: In this study, we aimed to evaluate the real-world efficacy and safety of camrelizumab and identify clinicolaboratory factors that predict treatment outcomes in patients with unresectable advanced, recurrent, or metastatic esophageal squamous cell carcinoma (ESCC) receiving camrelizumab.

Methods: Herein, 174 patients with unresectable advanced, recurrent, or metastatic ESCC treated with camrelizumab monotherapy (n = 30), camrelizumab + chemotherapy (CT; n = 91), and camrelizumab + radiotherapy (RT; n = 53) between October 1, 2019 and October 1, 2022 were included.

Results: The median follow-up time was 20 months (range, 1-34 months).

Postoperative tumor bed radiation versus T-shaped field radiation in the treatment of locally advanced thoracic esophageal squamous cell carcinoma: a phase IIb multicenter randomized controlled trial.

Background: Postoperative radiotherapy (PORT) is crucial for patients with thoracic locally advanced esophageal squamous cell carcinoma (LA-ESCC, pT3-4aN0-3M0) following esophagectomy. However, the appropriate radiation volume has not been well established. This study aimed to determine the optimal PORT volume for LA-ESCC patients.

Vertical bedrock shifts reveal summer water storage in Greenland ice sheet.

The Greenland ice sheet (GrIS) is at present the largest single contributor to global-mass-induced sea-level rise, primarily because of Arctic amplification on an increasingly warmer Earth. However, the processes of englacial water accumulation, storage and ultimate release remain poorly constrained. Here we show that a noticeable amount of the summertime meltwater mass is temporally buffered along the entire GrIS periphery, peaking in July and gradually reducing thereafter.

Structural basis for ligand recognition of the human hydroxycarboxylic acid receptor HCAR3.

Hydroxycarboxylic acid receptor 3 (HCAR3), a class A G-protein-coupled receptor, is an important cellular energy metabolism sensor with a key role in the regulation of lipolysis in humans. HCAR3 is deeply involved in many physiological processes and serves as a valuable target for the treatment of metabolic diseases, tumors, and immune diseases. Here, we report four cryoelectron microscopy (cryo-EM) structures of human HCAR3-Gi1 complexes with or without agonists: the endogenous ligand 3-hydroxyoctanoic acid, the drug niacin, the highly subtype-specific agonist compound 5c (4-(n-propyl)amino-3-nitrobenzoic acid), and the apo form.

CHAC1 blockade suppresses progression of lung adenocarcinoma by interfering with glucose metabolism via hijacking PKM2 nuclear translocation.

Patients with lung adenocarcinoma (LUAD) generally have poor prognosis. Abnormal cellular energy metabolism is a hallmark of LUAD. Glutathione-specific gamma-glutamylcyclotransferase 1 (CHAC1) is a member of the γ-glutamylcyclotransferase family and an unfolded protein response pathway regulatory gene.

A nomogram with Nottingham prognostic index for predicting locoregional recurrence in breast cancer patients.

Background: The Nottingham prognostic index (NPI) has been shown to negatively impact survival in breast cancer (BC). However, its ability to predict the locoregional recurrence (LRR) of BC remains still unclear. This study aims to determine whether a higher NPI serves as a significant predictor of LRR in BC.

Gonadal rematuration and sex-specific reproductive impairment in Manila clams under ocean acidification.

Ocean acidification (OA) can affect marine bivalves at various levels of biological organization. Yet, little effort has been devoted to understanding how OA affects the reproductive events of marine bivalves during multiple cycles of maturation. Here, we tested sex-specific reproductive responses of Manila clams (Ruditapes philippinarum) to OA during gonadal rematuration.

Advancing fluorescence tracing with 3D-2D spectral conversion: A mixed culture on microbial degradation mechanisms of DOM from a large-scale watershed.

Fluorescence tracing, known for its precision, rapid application, and cost-effectiveness, faces challenges due to the microbial degradation of dissolved organic matter (DOM) in aquatic environments, altering its original spectral fingerprint. This study conducted a 15-day microcosm experiment to examine the effects of biodegradation on the spectral properties of DOM from various sources: livestock excrement (EXC), urban sewage (URB), industrial wastewater (IND), and riparian topsoil (tDOM). Our findings show that while the spectral structures of DOM from different sources change during 15 days of microbial degradation, these changes do not overlap or interfere with each other.

Green workspace and urban health: exploring the impacts of industrial robotics in pollution emissions and public health.

Introduction: This study addresses a critical gap in understanding how technological advancements, specifically industrial robots, influence urban pollution emissions and public health. The rapid evolution of technology and changing working conditions significantly affect these areas, yet research has not extensively explored this domain.

Methods: Utilizing 2018 China Labor-force Dynamic Survey (CLDS) dataset, this study examines the impact of industrial robots on public health.

Structural basis for the ligand recognition and G protein subtype selectivity of kisspeptin receptor.

Kisspeptin receptor (KISS1R), belonging to the class A peptide-GPCR family, plays a key role in the regulation of reproductive physiology after stimulation by kisspeptin and is regarded as an attractive drug target for reproductive diseases. Here, we demonstrated that KISS1R can couple to the G pathway besides the well-known G pathway. We further resolved the cryo-electron microscopy (cryo-EM) structure of KISS1R-G and KISS1R-G complexes bound to the synthetic agonist TAK448 and structure of KISS1R-G complex bound to the endogenous agonist KP54.

Rapid and convenient screening method based on single-chain variable fragments for the detection of restricted monensin in chicken muscle.

A colloidal gold immunochromatographic assay (CGIA) based on single-chain variable fragments (scFvs) has been successfully developed for the detection of monensin (MON). Colloidal gold probes were conjugated to anti-MON scFvs through electrostatic interaction, with the conjugated objects serving as the visual signals. The detection lines were formed by capturing the antibody with MON-OVA.

[Clinical application of free peroneal artery leaflet perforator flap in postoperative complex defect of oral cancer].

To investigate the clinical effect of a segmented perforator flap of free peroneal artery to repair a complex defect after oral cancer. Forty-eight patients with oral cancer admitted to the Department of Oral and Maxillofacial Surgery of the First Affiliated Hospital of Bengbu Medical College from January 2018 to January 2022, including 8 of buccal cancer, 7 of floor cancer, 14 of tongue cancer, 5 of retromolar cancet, 9 of maxillary gingival cancer and 5 of mandibular gingival cancer. After lesion resection, 24 patients in the experimental group used the partial perforator flap to repair the defect, and 24 patients in the control group used the single flap to repair the defect.

Prognosis for local radical treatment in patients with esophageal squamous cell carcinoma with low-risk oligometastatic recurrence after curative resection: a retrospective cohort study.

Background: Patients with esophageal carcinoma (EC) with recurrent disease have a poor prognosis. A limited numbers of metastases, safely treatable with curative intent, diagnosed after curative esophagectomy may be defined as oligometastatic recurrence (OLR). However, the appropriate number of metastases and metastatic organs involved remains incompletely characterized.

The evolutionary features and internal logic of the one-vote veto system: A Nvivo analysis based on the policy texts of the three provinces in the east, middle and west.

Analyzing the evolutionary features and internal logic of the one-vote veto system in China over the past two decades is highly significant when considering reform and standardization. In order to conduct this analysis, the Nvivo 12 software was used to examine policy texts related to the one-vote veto issued by Fujian, Hubei, and Gansu provinces. Through a comparative analysis of keyword frequency statistics, policy text form, and content characteristics across the three provinces, it was discovered that governmental departments have experienced fundamental changes in their utilization of the one-vote veto system after 20 years of development.