A specific reverse complement sequence for distinguishing from other species.

Canine brucellosis is primarily caused by , but other species can also cause the disease. Identifying sequences specific to and establishing PCR assays that can distinguish between and other species is essential to determine the etiology of canine brucellosis and the source of infection and to achieve effective control. We analyzed the gaps and SNPs of genomes I and II from strain RM6/66 and strain 16M using the Mauve genome alignment software, and the specificity of each of these differential regions was analyzed by BLAST.

The Mechanism of Extraction of Peanut Protein and Oil Bodies by Enzymatic Hydrolysis of the Cell Wall.

Degradation of the peanut cell wall is a critical step in the aqueous enzymatic extraction process to extract proteins and oil bodies. Viscozyme L, a compound cell wall degrading enzyme, has been applied as an alternative to protease in the process of aqueous enzymatic extraction, but the mechanism of cell wall enzymolysis remains unclear. The present study aims to investigate the changes in cellulose, hemicellulose, and pectin content of the peanut cell wall hydrolyzed by Viscozyme L.

[A single-center retrospective analysis of 46 children with aerophagia].

Objective: To study the clinical features of aerophagia in children.

Meyjods: A retrospective analysis was performed on the medical data of 46 children with aerophagia who were diagnosed and treated in Children's Hospital Affiliated to Nanjing Medical University from October 2011 to September 2019.

Results: Among these 46 children, 15 (33%) had Tourette syndrome.

Peanut Oil Body Composition and Stability.

This study was aimed to assess the effect of membrane structure on the stability of peanut oil bodies extracted by enzyme-assisted extraction. The influence of pH, NaCl concentration, and temperature on the physicochemical properties of peanut oil bodies was characterized using ζ-potential and particle size. The results indicated that the peanut oil bodies had strong stability (ζ-potential, >20 mV) at pH values away from the isoelectric point (pH 4.

Effects of NaCl stress on stomatal traits, leaf gas exchange parameters, and biomass of two tomato cultivars.

To understand the mechanism underlying responses of stomatal traits, gas exchange parameters, and biomass of tomato plants to salt stress, two tomato cultivars (Shed and Alam) were treated by salt stress by adding NaCl (0.1 mol·L) to nutrition medium in environmental growth chambers for 90 days. Our results showed that salt stress substantially decreased the stomatal density, stomatal width, stomatal area, and stomatal area index of Shed by 32%, 45%, 25%, and 49%, respectively.

Extracellular ATP promotes stomatal opening of Arabidopsis thaliana through heterotrimeric G protein α subunit and reactive oxygen species.

In recent years, adenosine tri-phosphate (ATP) has been reported to exist in apoplasts of plant cells as a signal molecule. Extracellular ATP (eATP) plays important roles in plant growth, development, and stress tolerance. Here, extracellular ATP was found to promote stomatal opening of Arabidopsis thaliana in light and darkness.

[Mutation effect of ultra high pressure on microbe].

(Ultra) high pressure had many influences on microbe. It could regulate the expression of gene and protein, influence DNA's structure and function as well as change cell morphology and cell component. These effects not only make (ultra) high pressure to be applied into food sterilization, conserving and some processing, but also indicate it would play an important role in mutagenic breeding of microbe.

[The effect of IRESSA on H22 mouse hepatocellular carcinoma].

Objective: To investigate the effect of IRESSA (gefitinib, ZD1839) on H22 murine hepatocellular carcinoma.

Methods: Mice bearing H22 hepatocellular carcinoma were randomly divided into oral control group, Normal saline (NS) control group, cisplatin (CDDP) d1-5 group, CDDP d6-10 group, IRESSA group, IRESSA combined with CDDP early (IRESSA + CDDP d1-5) group, and IRESSA combined with CDDP lately (IRESSA + CDDP d6-10) group. IRESSA was given by daily gastrogavage for 10 days (day 1-day 10) at 100 mg/kg in body weight (BW).