[Speciation analysis and toxicity of arsenic in realgar bioleaching solution].

Objective: To analyze speciation and toxicity of arsenic in realgar bioleaching solution, and to explore its possible relation between speciation and toxicity.

Methods: Capillary zone electrophoresis (CZE) can be used quickly and simply onto the simultaneous separation and quantitative determination of the speciation of arsenic in realgar bioleaching solution. The effects of three different realgar bioleaching liquids on the acute toxicity level of rat were also investigated as well.

[Analysis of influenza type A(H1N1) antibody levels in different population in Gansu province].

Objective: To investigate influenza type A(H1N1) antibody levels in 3 different population which included common people, people recovered from be infected with influenza A(H1N1) and people vaccinated influenza type A(H1N1) vaccine in Gansu province in 2009.

Methods: Hemagglutination Inhibition (HI) test, HI test is positive when HI-titer > or = 1:40.

Results: The influenza type A(H1N1) antibody-positive rate of urban population were 3.

Biological extraction of realgar by Acidithiobacillus ferrooxidans and its in vitro and in vivo antitumor activities.

Acidithiobacillus ferrooxidans is a Gram-negative, chemolithoautotrophic bacterium involved in metal bioleaching. It is used for the extraction of coarse medical realgar, which is converted into an aqueous solution. To prove its feasibility as an anticancer drug candidate, extracted realgar (ER/Af) was evaluated for its antitumor activities both in vitro and in vivo.

Enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored DNA vaccine and a recombinant adenovirus.

Classical swine fever (CSF) - caused by the classical swine fever virus (CSFV) - is a fatal disease of pigs that is responsible for extensive losses to the swine industry worldwide. We had demonstrated previously that a prime-boost vaccination strategy using an alphavirus (Semliki Forest virus, SFV) replicon-vectored DNA vaccine (pSFV1CS-E2) and a recombinant adenovirus (rAdV-E2) expressing the E2 glycoprotein of CSFV induced enhanced immune responses in a mouse model. In this study, we evaluated further the efficacy of the heterologous prime-boost immunization approach in pigs, the natural host of CSFV.

[Hepatitis viruses infection situation in human population of the Gansu province].

Objective: To investigate the seroprevalence of hepatitis viruses in human population of Ganso province.

Methods: ELISA was used for detecting anti-HAY IgG, HBsAg/HBsAb, anti-HCV IgG and anti-HEV IgG of the serum samples. All sample were collected in four areas of KL, LT, HN and ZhL of Gansu province in 2008.

Aluminum uptake and disease resistance in Nicotiana rustica leaves.

The comparative effectiveness of aluminum hydroxide and aluminum chloride has been studied in the development of bacterial wilt infection on leaves of Nicotiana rustica cv. Gansu yellow flower. We have analyzed the changes of foliar H(2)O(2) content, as well as of non-enzymatic and enzymatic antioxidants under aluminum stress.

Enhanced expression of alternative oxidase genes is involved in the tolerance of rice (Oryza sativa L.) seedlings to drought stress.

Drought stress significantly enhanced the capacity of the alternative respiratory pathway and induced AOX1a and AOX1b transcripts in rice seedling leaves. The drought-stressed seedlings pretreated with the inhibitor of the alternative respiratory pathway, 1 mM salicylhydroxamic acid, had a lower level of relative water content than the seedlings either subjected to drought or salicylhydroxamic acid treatment alone. This observation suggests that the alternative respiratory pathway could play a role in the tolerance of rice seedlings to drought stress.

Type IV pili of Acidithiobacillus ferrooxidans are necessary for sliding, twitching motility, and adherence.

We used conventional methods to investigate the mechanism by which Acidithiobacillus ferrooxidans colonizes a solid surface by assessing pili-mediated sliding, twitching motility, and adherence. A. ferrooxidans slided to form circular oxidized zones around each colony.

[The inhibitory effects of Realgar bioleaching solution on S180 ascites-tumor and its toxicity].

Objective: To investigate the inhibitory effect of Realgar bioleaching solution (RBS) on tumor S180 cells line and estimate its toxicity, to provide experimental evidence for the further exploit of Realgar.

Methods: 24 hours after the models of ascites-tumor bearing mice were established, the mice were injected RBS once a day. The survival rates of S180 ascites-tumor bearing mice injected in RBS was studied, and the RBS acute toxicity of mice produced by oral, intraperitoneal or intravenous was evaluated by Drug Median Lethal Dose (LD50).

[Analysis of influenza surveillance from 2000 to 2007 in Gansu province].

Objective: To study the epidemical characteristics of influenza in Gansu province from 2000 to 2007, and to discuss the changes of the predominant strains of influenza virus. This study provide scientific basis for defending influenza effectively.

Methods: Influenza surveillance, collecting the pharyngeal swab specimens from influenza patients of national surveillance hospital and unsure influenza epidemic situations, inoculated on MDCK cell culture to obtain the influenza virus strains.

[Studies on chemical constituents from roots of Pterospermum heterophyllum].

Objective: To study the chemical constituents from the roots of Pterospermum heterophyllum.

Method: The chemical constituents of P. heterophyllum were isolated and purified by silica gel and sephadex LH-20 column chromatography.

Genomic and phenotypic heterogeneity of Acidithiobacillus spp. strains isolated from diverse habitats in China.

The genetic variability among 32 Chinese Acidithiobacillus spp. environmental isolates and four reference strains representing three recognized species of the genus Acidithiobacillus was characterized by using a combination of molecular methods, namely restriction fragment length polymorphisms of PCR-amplified 16S rRNA genes and 16S-23S rRNA gene intergenic spacers, repetitive element PCR, arbitrarily primed PCR and 16S rRNA gene sequence analyses. 16S rRNA gene sequences revealed that all Acidithiobacillus spp.

[Analysis of nonenzymic source of superoxide anion in pathogenic Xanthomonas oryzae pv. oryzae].

With electron spin resonance and chemical assay, the production of superoxide anion in the Xanthomonas oryzae pv. oryzae strain OS-14 suspension is extracellularly localized. It was found that superoxide anion produced in the filtrate.

[Evaluation of strand displacement amplification in the diagnosis of tuberculosis].

Objective: To study the clinical significance and reliability of strand displacement amplification (SDA) in detecting Mycobacterium tuberculosis complex.

Methods: SDA and fluorescence quantitative polymerase chain reaction (FQ-PCR) were employed to detect samples from 453 cases of tuberculosis, including 332 sputum samples, 78 samples of pleural effusion, and 43 samples of cerebrospinal fluid.

Results: In the 332 sputum samples, 131 were culture-positive, of which 110 samples (88 smear-positive) were Mycobacterium tuberculosis complex and 21 samples (20 smear-positive) were nontuberculous Mycobacteria.

Inter- and intraspecific genomic variability of the 16S-23S intergenic spacer regions (ISR) in representatives of Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans.

The complete sequences of 32 intergenic spacer regions (ISR) from Acidithiobacillus strains, including 29 field strains isolated from coal, copper, molybdenum mine wastes or sediment of different geoclimatic regions in China, reference strain ATCC19859 and the type strains of the two species were determined. These data, together with other sequences available in the GenBank database, were used to carry out the first detailed assessment of the inter- and intraspecific genomic variability of the ISR sequences and to infer phylogenetic relationships within the genus. The total length of the 16S-23S rRNA intergenic spacer regions of the Acidithiobacillus thiooxidans and Acidithiobacillus ferrooxidans strains ranged from 451 to 490 bp, and from 434 to 456 bp, respectively.

[Relationship between VEGF-C expression and nasopharyngeal carcinoma proliferation and metastasis].

Objective: To detect the expression of VEGF-C in nasopharyngeal carcinoma (NPC) and explore its relationship with proliferation and metastasis of NPC.

Methods: Biopsy specimens of 62 NPC patients were divided into 2 equal portions, one for immunohistochemical staining with VEGF-C polyclonal antibody by streptavidin peroxidase method, another for flow cytometry with Ki67 antibody to analyze the proliferation of tumors. The patients were followed up periodically, and then their 3-year survival and the cause of death were statistically analyzed.

[Exogenous PTEN enhances apoptosis in pancreas cancer cell line ASPC-1 induced by hypoxia].

Objective: To investigate the effect of exogenous phosphatase and tensin homologue deleted on chromosome ten (PTEN) on cell cycle, the expression of vascular endothelial growth factor (VEGF) and epidermal growth factor receptor (EGFR) proteins, and cellular proliferation ability in human pancreas cancer cell line (ASPC-1) exposed to normal oxygen or hypoxia 1% for 24 h were determined.

Methods: ASPC-1 cells were transfected in vitro with an eukaryotic expression plasmid (pEAK8) containing PTEN or not by lipofectin. Positive cell clones were selected, amplified and named ASPC-1-pEAK8-PTEN or ASPC-1-pEAK8 cells.

[Study on immobilization of Acidithiobacillus ferrooxidans using PVA-Ca(NO3)2 method].

A new cell immobilization technique utilizing the complex of PVA solution and sodium alginate solution as entrapment medium is reported. The mixture of Acidithiobacillus ferrooxidans suspension and the entrapment complex were extruded into the solution of Ca(NO3)2 (1% - 5%) to form beads, then the beads were frozen at -20 degrees C for 1d and thawed at room temperature. This method can simultaneously eliminate the agglomeration of PVA beads and the biological toxicity of H3 BO31.

[Short hairpin RNA silences Pin1 and affects proliferation and apoptosis in HeLa cell line].

Objective: To study the effect of short hairpin RNA (shRNA) on the expression of Pin1 mRNA and protein and its influence on the proliferation and apoptosis in HeLa cell lines.

Methods: The recombinant plasmid pSIREN-Pin1 expressing Pin1-targeted shRNA was constructed and then transfected into cervical cancer cell lines by lipofectamine (HeLa/p-shRNA), the control vector pSIREN-Con (HeLa/p-Con) and culture media (HeLa) as control, respectively. The Pin1 mRNA and protein expression were detected by RT-PCR and immunoblotting.

[Expression and function of AOX1 gene family during the greening of etiolated rice seedlings].

Under constant light, increases in V(t) (total respiration rate), V(alt) (capacity of alternative pathway), and V(alt)/V(t) ration values (Figs.1-3) in fully etiolated rice seedlings leaves were founded. Northern hybridization with special probes for rice AOX1a, AOX1b and AOX1c revealed that the AOX1c mRNA was mainly present under constant light (Fig.

[Effects of drought stress on cyanide-resistant respiration and metabolism of reactive oxygen in wheat seedling].

The changes in cyanide-resistant respiration and metabolism of active oxygen species in drought-sensitive and drought-resistant wheat seedlings under water stress were studied. In general, drought stress decreased the level of cyanide-resistant respiration and AOX1 mRNA content in wheat seedling leaves. But the drought-resistant cultivars, which had much higher level of cyanide-resistant respiration and AOX1 mRNA content than the drought-sensitive ones, presented an ability to adapt to mild drought stress.

Dihydropyrrolopyrazole transforming growth factor-beta type I receptor kinase domain inhibitors: a novel benzimidazole series with selectivity versus transforming growth factor-beta type II receptor kinase and mixed lineage kinase-7.

Novel dihydropyrrolopyrazole-substituted benzimidazoles were synthesized and evaluated in vitro as inhibitors of transforming growth factor-beta type I receptor (TGF-beta RI), TGF-beta RII, and mixed lineage kinase-7 (MLK-7). These compounds were found to be potent TGF-beta RI inhibitors and selective versus TGF-beta RII and MLK-7 kinases. Benzimidazole derivative 8b was active in an in vivo target (TGF-beta RI) inhibition assay.

[Expression and clinical significance of Pin1 and Cyclin D1 in cervical cancer cell lines and cervical epithelial tissues].

Background & Objective: Peptidyl-prolyl cis/trans isomerase Pin1 is prevalently overexpressed in human cancers. Up-regulation of Pin1 elevates the expression of Cyclin D1, and plays an important role in tumorigenesis and tumor progression. This study was to investigate the expression and clinical significance of Pin1 and Cyclin D1 in cervical cancer cell lines and cervical epithelial tissues.