Enhancing fat graft survival: thymosin beta-4 facilitates mitochondrial transfer from ADSCs via tunneling nanotubes by upregulating the Rac/F-actin pathway.

Autologous fat grafting is a widely used technique in plastic and reconstructive surgery, but its efficacy is often limited by the poor survival rate of transplanted adipose tissue. This study aims to enhance the survival of fat grafts by investigating the role of thymosin beta-4 (Tβ4) in facilitating mitochondrial transfer from adipose-derived stem cells (ADSCs) to adipocytes and newly formed blood vessels within the grafts via tunneling nanotubes (TNTs). We demonstrate that Tβ4 upregulates the Rac/F-actin pathway, leading to an increased formation of TNTs and subsequent transfer of mitochondria from ADSCs.

Perioperative ultrasound screening of lower extremity veins is effective in the prevention of fatal pulmonary embolism in orthopedic patients.

Patients at high risk of deep vein thrombosis are recommended to undergo lower-extremity ultrasonography to screen for pulmonary embolism (PE); however, there are few reports on whether this can effectively reduce the occurrence of fatal pulmonary embolism (FPE). This study aimed to assess the risk factors associated with PE and to investigate whether perioperative ultrasound screening of lower extremity veins in orthopedic patients can effectively reduce the incidence of FPE. We enrolled 137 patients with PE who underwent orthopedic surgery between 2013 and 2020.

Toxicity Risk Assessment of Clethodim Enantiomers in Rats and Mice: Insights from Stereoselective Effects.

Clethodim is a chiral herbicide with two enantiomers. The herbicidal activity of (-)-clethodim is 1.3-2.

Endurance training enhances skeletal muscle mitochondrial respiration by promoting MOTS-c secretion.

The mitochondrial open reading frame of 12S rRNA-c (MOTS-c) is a biologically active mitochondria-derived peptide. However, the relationship between MOTS-c, skeletal muscle mitochondrial function, and endurance exercise adaptations is unknown. Here, we tested indices such as maximal oxygen uptake and serum MOTS-c levels in marathon runners and sedentary subjects.

Optical Evidence of Interfacial Strain-Induced Ferroelectric Tuning and Enhancement in CuInPS via Ferroelectric Substrate.

The precise domain control in ferroelectric CuInPS (CIPS) remains challenging. A promising approach is by interfacing CIPS with the ferroelectric layer, but interface-driven ferroelectricity tunning mechanism remains unclear. Here, the demonstration of interfacial strain-induced ferroelectric tuning and enhancement in CIPS via ferroelectric substrate is reported by photoluminescence (PL) spectroscopy, combined with piezoresponse force microscopy (PFM) and density functional theory (DFT) calculations.

Highly electroactive mixed-valence-MoO as an electrochemical sensing for effective detection of p-nitrophenol based on the synergistic effect of valence change and oxygen vacancy.

Transition metal oxides (TMOs) can effectively improve the performance of electrochemical detection due to their unique electronic structure and redox properties. However, the lack of reproducibility and the electrical activity of TMOs prepared from conventional preparation methods limit their further development. In this work, amorphous MoO with reductive Mo(V) was successfully synthesized by one-step electrodeposition, and it has excellent detection performance for p-nitrophenol (PNP).

SerpinB1 targeting safeguards against pathological cardiac hypertrophy and remodelling by suppressing cardiomyocyte pyroptosis and inflammation initiation.

Aims: While the pivotal role of inflammation in pathological cardiac hypertrophy and remodelling is widely acknowledged, the mechanisms triggering inflammation initiation remain largely obscure. This study aims to elucidate the role and mechanism of serpin family B member 1 (SerpinB1) in pro-inflammatory cardiomyocyte pyroptosis, heart inflammation, and cardiac remodelling.

Methods And Results: C57BL/6J wild-type, inducible cardiac-specific SerpinB1 overexpression or knockout mice underwent transverse aortic constriction (TAC) surgery.

Spin-Orbit-Locking Vectorial Metasurface Holography.

Vectorial metasurface holography, allowing for independent control over the amplitude, phase, and polarization distribution of holographic images enabled by metasurfaces, plays a crucial role in the realm of optical display, optical, and quantum communications. However, previous research on vectorial metasurface holography has typically been restricted to single degree of freedom input and single channel output, thereby demonstrating a very limited modulation capacity. This work presents a novel method to achieve multi-channel vectorial metasurface holography by harnessing spin-orbit-locking vortex beams.

Analyzing the Effects of a Repeated Reading Intervention on Reading Fluency With Generalized Linear Mixed Models.

In the United States, only approximately one-third of students read at or above proficiency level. Moreover, the difference in reading proficiency of students is severely disproportionate for students with disabilities compared to students without disabilities. Reading fluency is a precursor for academic success and one of the six aspects of reading that contributes to effective reading skills.

Using Generalized Linear Mixed Models in the Analysis of Count and Rate Data in Single-case Eperimental Designs: A Step-by-step Tutorial.

Generalized linear mixed models (GLMMs) have great potential to deal with count and rate data in single-case experimental designs (SCEDs). However, applied researchers face challenges to apply such an advanced approach in their own studies. Hence, our study aimed to provide a tutorial and demonstrate a step-by-step procedure of using GLMMs to handle SCED count and rate outcomes.

Mechanistic insights into the regression of atherosclerotic plaques.

Atherosclerosis is a major contributor to cardiovascular diseases and mortality globally. The progression of atherosclerotic disease results in the expansion of plaques and the development of necrotic cores. Subsequent plaque rupture can lead to thrombosis, occluding blood vessels, and end-organ ischemia with consequential ischemic injury.

Inflammatory and Nutritional Markers as Indicators for Diagnosing and Assessing Disease Activity in MS and NMOSD.

Background: Inflammation and nutritional markers have recently gained recognition for their roles in the fabrication of cognitive control centers demyelinating illnesses. Inflammatory indices such as the neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), systemic immune-inflammatory index (SII), and systemic inflammatory response index (SIRI), along with nutritional markers like albumin (ALB), hemoglobin (HB), and body mass index (BMI), may predict disease occurrence. However, their potential in evaluating diseases such as multiple sclerosis (MS) and neuromyelitis optica spectrum disorder (NMOSD) remains unexplored.

Nicotinamide N-methyltransferase negatively regulates metastasis-promoting property of cancer-associated fibroblasts in lung adenocarcinoma.

Background: Recurrence and metastasis remain significant challenges in lung adenocarcinoma (LUAD) after radical resection. The mechanisms behind the recurrence and metastasis of LUAD remain elusive, and deregulated cellular metabolism is suspected to play a significant role. This study explores the metabolic and epigenetic regulation mediated by nicotinamide N-methyl transferase (NNMT) in LUAD.

Salidroside ameliorates cerebral ischemic injury and regulates the glutamate metabolism pathway in astrocytes.

Background And Aim: Salidroside (SA) is the main active component of L., with potential in treating cardiovascular and cerebrovascular diseases and cerebral ischemia. However, its efficacy and mechanism in cerebral ischemia remain unclear, particularly regarding its effect on glutamate (Glu) metabolism.

Risk factors for healing failure after arthroscopic rotator cuff repair in small to medium-sized tears: a retrospective cohort study.

Objectives: To identify risk factors for tendon healing failure following arthroscopic rotator cuff repair (ARCR) in patients with small to medium-sized rotator cuff tears (RCTs).

Methods: A retrospective study was conducted on 320 patients with RCTs who underwent arthroscopic repair between June 2018 and June 2021. All patients had at least 2 years of postoperative follow-up, with MRI scans at the final assessment.

Seasonal variations and temporal instability of motorcyclist injury severity in Cambodia: Analyses based on a random parameter logit model with heterogeneity in means and variances.

Motorcycles are a prevalent mode of transportation in countries like Cambodia that experience distinct rainy and dry seasons. However, the safety concerns associated with motorcycling in this region have not been thoroughly investigated. This study addresses this research gap by examining the severity of motorcyclist injuries in Cambodia, considering the potential variations across seasons and the fluctuations in contributing factors over time.

Long-Term Survival in Grafted Sites of Dental Implants.

Objective: Vertical and horizontal bone insufficiency in both the maxilla and mandible has long been a contraindication for implants. This review provides an overview of the long-term survival rates of implants in augmented alveolar bone after the guided bone regeneration (GBR) technique.

Results: GBR in a combination of allogeneic or xenogeneic graft material with a barrier membrane and simultaneous or delayed placement of dental implants has proven to be a very effective and feasible treatment option in long-term studies up to 35 years.

Cell-microsphere based living microhybrids for osteogenesis regulating to boosting biomineralization.

Biomineralization-based cell-material living composites showed great potential for living materials construction and cell regulation. However, cells in scaffolds with unconnected pores usually induce confined nutrient transfer and cell-cell communications, affecting the transformation of osteoblasts into osteocytes and the mineralization process. Herein, the osteoblast-materials living hybrids were constructed with porous PLLA microspheres using a rational design, in which cell-based living materials presented an improved osteoblast differentiation and mineralization model using rationally designed cell-microsphere composites.

Basic-Functionalized Ionic Porous Organic Polymers: Triple Roles in One for Highly Efficient and Recyclable Carboxylative Cyclization of CO under Mild Conditions.

The transformation of carbon dioxide (CO) into high-value chemicals is a significant step towards achieving the goal of "carbon neutrality". α-methylene cyclic carbonate, as an intermediate for the synthesis of many important organic compounds, is widely employed in industrial productions. In this work, a series of ionic porous organic polymers (IPOPs) with different basic-functionalized anions were successfully synthesized and adjusted to have certain BET surface areas and high contents of ion sites by post-modification.

Mussel-inspired highly adhesive carrageenan-based coatings with self-activating enhanced activity for meat preservation.

In order to solve the problem of poor adhesion of polysaccharide coatings in meat storage and inconvenient secondary spraying, which leads to poor preservation effect, this study was inspired by the property of mussels to adhere firmly to surfaces and design a bioactive composite coating. Here, curcumin-loaded zein nanoparticles (CZ NPs) were successfully prepared and incorporated into carrageenan-based biocomposite coatings for chilled meat preservation. The prepared curcumin-zein-riboflavin-carrageenan (CZRC) coating featured smooth spherical morphology and the solubility of hydrophobic substances, the adhesion and stability of the composite coating were respectively improved to 3.