Getah virus triggers ROS-mediated autophagy in mouse Leydig cells.

Introduction: Getah virus (GETV) is a zoonotic virus transmitted via a mosquito-vertebrate cycle. While previous studies have explored the epidemiology and pathogenicity of GETV in various species, its molecular mechanisms remain largely unexplored.

Methods: This study investigated the impact of GETV infection and associated molecular mechanisms on reactive oxygen species (ROS) and autophagy levels in mouse Leydig cells both and .

One Health Approach of Enterococcal Population Structure and Antibacterial Resistance Along the Food Chain - Four PLADs, China, 2015-2022.

Introduction: Enterococci are considered opportunistic pathogens. However, they can serve as a reservoir of antibacterial resistance (ABR) traits and transfer these to humans through the food chain.

Methods: Antibiotic susceptibility testing and multilocus sequence typing were used to characterize the ABR and population structure of 488 enterococcal isolates recovered along the food chain from four provincial-level administrative divisions (PLADs) in China.

Micro/Nano Hierarchical Dumbbell-like and Micropapillae Structure Improves Light Absorption and Facilitates Anti-icing/Deicing Performance.

The emergence of ice formation and accretion presents significant challenges, catalyzing an urgent need for clean and efficient anti-icing solutions. Solar energy, a powerful and sustainable resource, can be integrated with the micronano-structured superhydrophobic surface to enhance anti-icing and deicing performance through the solar photothermal effect, overcoming the limitations of a traditional superhydrophobic surface. Herein, inspired by bamboo and lotus leaves, a synergetic photothermal anti-icing superhydrophobic surface (PASS) has been developed.

In situ formation of biomolecular condensates as intracellular drug reservoirs for augmenting chemotherapy.

Biomolecular condensates, which arise from liquid-liquid phase separation within cells, may provide a means of enriching and prolonging the retention of small-molecule drugs within cells. Here we report a method for the controlled in situ formation of biomolecular condensates as reservoirs for the enrichment and retention of chemotherapeutics in cancer cells, and show that the approach can be leveraged to enhance antitumour efficacies in mice with drug-resistant tumours. The method involves histones as positively charged proteins and doxorubicin-intercalated DNA strands bioorthogonally linked via a click-to-release reaction between trans-cyclooctene and tetrazine groups.

Antimicrobial Resistance and Genomic Characterization of and Isolated from Retail Chickens in Beijing, China.

Objective species are the main causes of foodborne illness worldwide, posing significant threats to public health. This study aimed to investigate the antibiotic resistance and genomic characterization of / from retail chickens in Beijing. Methods Antimicrobial susceptibility testing was conducted on 126 / isolated from retail chickens in Beijing, following CLSI protocols.

Porcine reproductive and respiratory syndrome virus infects the reproductive system of male piglets and impairs development of the blood-testis barrier.

Porcine reproductive and respiratory syndrome virus (PRRSV) causes a highly contagious disease that threatens the global swine industry. Recent studies have focused on the damage that PRRSV causes to the reproductive system of male pigs, although pathological research is lacking. Therefore, we examined the pathogenic mechanisms in male piglets infected with PRRSV.

Electro-Osmotic Flow and Mass Transfer through a Rough Microchannel with a Modulated Charged Surface.

In this paper, we investigate the electro-osmotic flow (EOF) and mass transfer of a Newtonian fluid propelled by a pressure gradient and alternating current (AC) electric field in a parallel microchannel with sinusoidal roughness and modulated charged surfaces. The two-wall roughness is described by in-phase or out-of-phase sine functions with a small amplitude δ. By employing the method of perturbation expansion, the semi-analytical solutions of the Poisson-Boltzmann (P-B) equation based on the Debye-Hückel approximation and the modified Navier-Stokes (N-S) equation are obtained.

Metabolomic and transcriptomic analysis of the flavonoid biosynthesis pathway in (Sieb. et Zucc.) Maxim. from distinct locations.

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Prevalence and characterization of foodborne pathogens isolated from fresh-cut fruits and vegetables in Beijing, China.

Pre-cut fresh fruits and vegetables are highly appealing to consumers for their convenience, however, as they are highly susceptible to microbial contamination in processing, the potential risks of foodborne illnesses to public health are not negligible. This study aimed to assess the prevalence, antibiotic susceptibility and molecular characteristics of major foodborne pathogens (Listeria monocytogenes, Escherichia coli, Staphylococcus aureus and Salmonella) isolated from fresh-cut fruits and vegetables in Beijing, China. 86 stains were isolated from 326 samples, with S.

Antimicrobial susceptibility and genomic characterization of Vibrio parahaemolyticus isolated from aquatic foods in 15 provinces, China, 2020.

Prevalent in marine, estuarine and coastal environments, Vibrio parahaemolyticus is one of the major foodborne pathogens which can cause acute gastroenteritis through consumption of contaminated food. This study encompassed antimicrobial resistance, molecular characteristics and phylogenetic relationships of 163 V. parahaemolyticus isolated from aquatic foods across 15 provinces in China.

School physical activity modes on BMI among adolescents with overweight or obesity: The mediation role of participation willingness.

Objectives: To analyze the mode of school physical activity, the relationship with obesity, and participation willingness and behavior in the relationship between modes of school physical activity and BMI in students with overweight or obesity.

Design: Cross-sectional study.

Sample: Data from the 2014 Physical Fitness and Health Surveillance of Chinese School Students was used, wherein physical measurements and questionnaires of 3476 middle school students were collected.

A DNA/Upconversion Nanoparticle Complex Enables Controlled Co-Delivery of CRISPR-Cas9 and Photodynamic Agents for Synergistic Cancer Therapy.

Photodynamic therapy (PDT) depends on the light-irradiated exciting of photosensitizer (PS) to generate reactive oxygen species (ROS), which faces challenges and limitations in hypoxia and antioxidant response of cancer cells, and limited tissue-penetration of light. Herein, a multifunctional DNA/upconversion nanoparticles (UCNPs) complex is developed which enables controlled co-delivery of CRISPR-Cas9, hemin, and protoporphyrin (PP) for synergistic PDT. An ultralong single-stranded DNA (ssDNA) is prepared via rolling circle amplification (RCA), which contains recognition sequences of single guide RNA (sgRNA) for loading Cas9 ribonucleoprotein (RNP), G-quadruplex sequences for loading hemin and PP, and linker sequences for combining UCNP.

Convergence of resistance and evolutionary responses in Escherichia coli and Salmonella enterica co-inhabiting chicken farms in China.

Sharing of genetic elements among different pathogens and commensals inhabiting same hosts and environments has significant implications for antimicrobial resistance (AMR), especially in settings with high antimicrobial exposure. We analysed 661 Escherichia coli and Salmonella enterica isolates collected within and across hosts and environments, in 10 Chinese chicken farms over 2.5 years using data-mining methods.