Genetic Variants Affecting FADS2 Enzyme Dynamics and Gene Expression in Cogenetic Oysters with Different PUFA Levels Provide New Tools to Improve Unsaturated Fatty Acids.

Long-chain polyunsaturated fatty acids (LC-PUFAs) are crucial for human health and cannot be produced internally. Bivalves, such as oysters, serve as valuable sources of high-quality PUFAs. The enzyme fatty acid desaturase (FADS) plays a key role in the metabolism of LC-PUFAs.

Comparative transcriptome analysis of albino northern snakehead (Channa argus) reveals its various collagen-related DEGs in caudal fin cells.

The albino northern snakehead (Channa argus) is an aquaculture species characterized by heritable albino body color, in contrast to the typical coloration. Additionally, there are gray- and golden-finned individuals, which exhibit distinct coloration in their caudal fins. We performed RNA-seq to profile the transcriptome of caudal fin tissues in albino gray-finned and golden-finned C.

Heterophilous distribution propagation for Graph Neural Networks.

Graph Neural Networks (GNNs) have achieved remarkable success in various graph mining tasks by aggregating information from neighborhoods for representation learning. The success relies on the homophily assumption that nearby nodes exhibit similar behaviors, while it may be violated in many real-world graphs. Recently, heterophilous graph neural networks (HeterGNNs) have attracted increasing attention by modifying the neural message passing schema for heterophilous neighborhoods.

Mechanistic insights into Quetiapine's Protective effects on cognitive function and synaptic plasticity in epileptic rats.

The study aimed to examine the effects of Quetiapine, an atypical antipsychotic medication with purported neuroprotective qualities, on cognitive function and synaptic plasticity in epileptic rats. This investigation also sought to elucidate the mechanisms by which quetiapine influences the activity of the cyclic adenylate response element binding protein (CREB)/brain-derived neurotrophic factor (BDNF) signaling pathway and metallomatrix proteinase-9 (MMP9) expression in the context of epilepsy. The epileptic model was induced in rats through the administration of pilocarpine, with normal rats serving as the control group.

Inhibiting FGFR by toadflax reverses erlotinib resistance in nonsmall cell lung cancer.

This study aims to demonstrate the effect of toadflax (bufalin) on erlotinib resistance in nonsmall cell lung cancer (NSCLC) by inhibiting the fibroblast growth factor receptor (FGFR). The microfluidic mobility transferase and caliper mobility-shift assays were employed to detect the FGFR inhibition by bufalin and the binding reversibility. Further, the inhibitory effects of bufalin were determined in HCC827 and HCC827/ER cells in vitro, investigating relative FGFR overexpression by quantitative reverse transcriptase-PCR (RT-qPCR) and FGFR downstream proteins, that is, FGFR substrate 2 (FRS2), extracellular signal-regulated kinase (ERK), and S6 by western blot analysis.

[Simultaneous Removal of Antibiotic-resistant Bacteria, Genes, and Inhibition of Horizontal Transfer using Vis-rGO-CNCF-enhanced Peroxymonosulfate Activation Process].

As emerging contaminants, antibiotic-resistant bacteria （ARBs） and antibiotic-resistant genes （ARGs） pose a serious threat to human health and ecological security. Here, a reduced graphene oxide and g-CN co-doped copper ferrite （rGO-CNCF） were synthesized. The composite material was characterized using XRD, FTIR, XPS, SEM-EDS, TEM, and DRS analysis methods, and a visible-light-assisted rGO-CNCF-activated PMS system was constructed for the removal of ARB and ARGs in water.

Synthesis of ultra-large diameter graphene oxide flakes from natural flake graphite.

Graphene and its derivatives are widely used in various fields due to their unique two-dimensional lamellar structure. This study aims to synthesize ultra-large graphene oxide (GO) sheets from natural flake graphite and investigate the factors influencing their size. Using a two-intercalation method based on the modified Hummers' method, we address the challenge of intercalating large-diameter graphene oxide by employing a secondary intercalation technique.

Genotype-by-Environment Effects of Cis-Variations in the Atgl Promoter Mediate the Divergent Pattern of Phenotypic Plasticity for Temperature Adaptation in Two Congeneric Oyster Species.

Phenotypic plasticity plays an essential role in adaptive evolution. However, the molecular mechanisms of how genotype-by-environment interaction (G × E) effects shape phenotypic plasticity in marine organisms remain poorly understood. The crucial temperature-responsive trait triacylglycerol (TAG) content and its major gene adipose triglyceride lipase (Atgl) expression have divergent plastic patterns in two congeneric oyster species (Crassostrea gigas and Crassostrea angulata) to adapt to relative-cold/northern and relative-warm/southern habitats, respectively.

Creating Single-Crystalline β-CaSiO for High-Performance Dielectric Packaging Substrate.

β-CaSiO based glass-ceramics are among the most reliable materials for electronic packaging. However, developing a CaSiO glass-ceramic substrate with both high strength (>230 MPa) and low dielectric constant (<5) remains challenging due to its polycrystalline nature. The present work has succeeded in synthesizing single-crystalline β-CaSiO for a high-performance glass-ceramic substrate.

A feasible approach to chromophores removal and color reduction in industrial lignin via deep eutectic solvent/isopropanol treatment.

The dark coloration of industrial lignin significantly limits its potential for applications in high-value products. This work reported a practical strategy for lignin color reduction through the synergistic treatment of acidic deep eutectic solvent and isopropanol (DES-IPA). The results showed that the DES-IPA treatment could effectively remove the p-coumarate units and methoxy groups in lignin.

Reduced-dose deep learning iterative reconstruction for abdominal computed tomography with low tube voltage and tube current.

Background: The low tube-voltage technique (e.g., 80 kV) can efficiently reduce the radiation dose and increase the contrast enhancement of vascular and parenchymal structures in abdominal CT.

Pan-cancer analysis reveals the prognostic and immunomodulatory potential of super-enhancer-induced ANGPT2 and experimental validation in colorectal cancer.

Background: ANGPT2 plays important roles in cancer development. However, there is still no systematic analysis of ANGPT2 in pan-cancer.

Methods: In this paper, we conducted a pan-cancer analysis to investigate the characteristics of ANGPT2.

Diet shapes and maintains the personalized native gut microbiomes in mice.

Background: The gut microbiome plays a critical role in human health and disease. Different dietary backgrounds play an important role in the uniqueness and diversity of the gut microbiota in different individuals, which promotes heterogeneity in disease phenotypes and treatment responses. Here, we explored how diet affects the composition and function of the native gut microbiome of model mice, based on the shotgun metagenomic and metabolomic, by analyzing the gut microbiome of C57B/6J mice in different dietary backgrounds.

Improving the storage quality of sweet cherry by natamycin nanoparticles/chitosan coating.

In this study, natamycin nanoparticles were prepared by anti-solvent method, and natamycin nanoparticles/chitosan coating was prepared and investigated for the preservation of sweet cherry. The results of particle size showed that the prepared natamycin nanoparticles were nano-sized, with a particle size of 209 ± 10 nm. The results of scanning electron microscope (SEM) showed that the apparent morphology was a uniform spherical structure.

Transcriptomics reveals crowding stress inhibit the immune defense of the head kidney of the pearl gentian grouper juvenile through NF-κB signal pathway.

Crowding stress is a significant welfare factor affecting aquatic animals in recirculating aquaculture systems. Little is known regarding the influence of prolonged crowding stress on the immunity of juvenile pearl gentian groupers. However, research exploring the potential mechanisms through which crowding stress affects fish immune function is limited.

A randomized double-blinded study assessing the effect of different doses of transnasal dexmedetomidine on the median effective concentration of ropivacaine for a caudal block.

Background: Perineural administration of dexmedetomidine (PN-DEX) can enhance the efficacy of local anesthetics used in regional nerve blocks while decreasing the median effective concentration (EC50) of these anesthetics. Intranasal administration of dexmedetomidine (IN-DEX) is more accessible for sedation during regional anesthesia because of its non-invasive systemic administration and demonstrates synergism with local anesthetic. However, it remains unclear whether IN-DEX affects the EC50 of local anesthetics used in caudal blocks.

Effects of volatile anaesthetics on incidence of postoperative depression and anxiety symptoms in elderly patients: A retrospective analysis of a prospective cohort study.

Studies have suggested volatile anaesthetics may alleviate depression and anxiety symptoms in patients. However, there is a paucity of research in this area. We wanted to determine the association between volatile anaesthetics and depression/anxiety symptoms in elderly patients within 7 days after surgery.

Chromosome-level genome assembly of the Suminoe oyster Crassostrea ariakensis in south China.

The Suminoe oyster Crassostrea ariakensis (Fujita, 1913) is one of the most important ecological and fishery bivalve mollusks with a worldwide distribution. Here, we reported an improved high-quality chromosomal-level genome assembly of C. ariakensis inhabiting the South China Sea, using Nanopore technology, Illumina sequencing, and high-throughput chromosomal conformation capture analysis.

Application of Imaging Algorithms for Gas-Water Two-Phase Array Fiber Holdup Meters in Horizontal Wells.

The flow dynamics of low-yield horizontal wells demonstrate considerable complexity and unpredictability, chiefly attributable to the structural attributes of the wellbore and the interplay of gas-water two-phase flow. In horizontal wellbores, precisely predicting flow patterns using conventional approaches is often problematic. Consequently, accurate monitoring and analysis of water holdup in gas-water two-phase flows are essential.

Multi-Frame Vibration MEMS Gyroscope Temperature Compensation Based on Combined GWO-VMD-TCN-LSTM Algorithm.

This paper presents a temperature compensation model for the Multi-Frame Vibration MEMS Gyroscope (DMFVMG) based on Grey Wolf Optimization Variational Mode Decomposition (GWO-VMD) for denoising and a combination of the Temporal Convolutional Network (TCN) and the Long Short-Term Memory (LSTM) network for temperature drift prediction. Initially, the gyroscope output signal was denoised using GWO-VMD, retaining the useful signal components and eliminating noise. Subsequently, the denoised signal was utilized to predict temperature drift using the TCN-LSTM model.

Comparative Analysis of PGRP Family in Polymorphic Worker Castes of .

Peptidoglycan recognition proteins (PGRPs) are a class of pattern recognition receptors (PRRs) that activate the innate immune system in response to microbial infection by detection of peptidoglycan, a distinct component of bacterial cell walls. Bioinformatic studies have revealed four PGRPs in the red imported fire ant ; nonetheless, the mechanism of the immune response of induced by pathogens is still poorly understood. The peptidoglycan recognition protein full-length cDNA (designated as -) from was used in this investigation.

Melatonin improves cadmium tolerance in Salix viminalis by enhancing amino acid and saccharide metabolism, and increasing polyphenols.

As a short-rotation woody plant, Salix viminalis has the potential for phytoremediation of cadmium (Cd), but it has poor tolerance to high Cd concentrations. Melatonin (MEL), a candidate bio-promoter, was considered to play an active role in plant responses to Cd. However, the molecular mechanism by which MEL regulates metabolic processes in plants to defend against Cd stress remain unclear.