Feasibility and preliminary accuracy of high-resolution imaging of the liver and pancreas using FNA compatible microendoscopy (with video).

Background: EUS-guided FNA is one of the few techniques that can obtain cells and tissue from the liver and pancreas. However, the technique remains vulnerable to poor specimen quality and sampling error.

Objective: To evaluate the ability of a high-resolution microendoscope (HRME) to visualize the cellular and architectural features of normal and malignant liver and pancreatic tissue ex vivo, to assess the ability of endosonographers to identify normal and neoplastic tissue by using HRME images, and to demonstrate preliminary technical feasibility of in vivo HRME imaging via EUS fine-needle puncture (FNP).

Dual-mode reflectance and fluorescence near-video-rate confocal microscope for architectural, morphological and molecular imaging of tissue.

We have developed a near-video-rate dual-mode reflectance and fluorescence confocal microscope for the purpose of imaging ex vivo human specimens and in vivo animal models. The dual-mode confocal microscope (DCM) has light sources at 488, 664 and 784 nm, a frame rate of 15 frames per second, a maximum field of view of 300 x 250 mum and a resolution limit of 0.31 mum laterally and 1.

Demodex musculi in the Skin of Transgenic Mice.

Although infestations by a number of Demodex mite species have been described in mice, the occurrence of Demodex musculi infestation was last reported by Hirst in 1917. This communication describes the occurrence of D. musculi infestation in two lines of transgenic mice and their F1-hybrid offspring.

Perineal swellings in two strains of mice.

Seven adult male mice of two different strains and from two different animal housing facilities were submitted for necropsy to evaluate unilateral or bilateral swellings in the ventral perineal area. On dissection, the swellings were oval to spherical cystic structures located near the base of the penis. Striated muscle and a delicate stroma encapsulated all cystic structures.