Optimal conditions for [3H]apomorphine binding and anomalous equilibrium binding of [3H]apomorphine and [3H]spiperone to rat striatal membranes: involvement of surface phenomena versus multiple binding sites.

I. Binding of [3H] apomorphine to dopaminergic receptors in rat striatum was most reproducible and clearly detectable when incubations were run at 25 degrees C in Tris-HCl buffer, pH 7.5, containing 1 mM-EDTA and 0.

Serotoninergic receptors in brain tissue: properties and identification of various 3H-ligand binding sites in vitro.

In vitro binding studies to serotoninergic receptors were performed using 3H-LSD, 3H-5-HT and 3H-spiperone. An overview is given on findings using these three ligands with respect to the following: (1) Localization of specific binding sites, i.e.