Publications by authors named "Leyla Fahim"
Non-canonical roles for growth factors in the nucleus have been previously described, but their mechanism of action and biological roles remain enigmatic. Platelet-derived growth factor B (PDGFB) can drive formation of low-grade glioma and here we show that it localizes to the nucleus of human glioma cells where it binds chromatin to preserve genome stability and cell lineage. Failure of PDGFB to localize to the nucleus leads to chromosomal abnormalities, aberrant heterochromatin architecture and accelerated tumorigenesis.
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- Ribonucleoprotein (RNP) condensates create distinct liquid phases that make it hard to study their functions versus the surrounding environment.
- Researchers implemented fluorescence lifetime imaging microscopy (FLIM) alongside phasor plot filtering to better differentiate these condensates and assess protein interactions using FLIM-Förster resonance energy transfer (FRET).
- They found that interactions among decapping complex subunits in P-bodies change based on conditions, particularly observing altered interactions during oxidative stress, highlighting the dynamic nature of these protein networks in live cells.