Transcriptional analysis of Pseudomonas aeruginosa infected Caenorhabditis elegans.

Here, we describe a protocol for the extraction of total RNA from C. elegans infected with P. aeruginosa.

Draft genomes of 12 host-adapted and environmental isolates of Pseudomonas aeruginosa and their positions in the core genome phylogeny.

Pseudomonas aeruginosa is a Gram-negative opportunistic pathogen particularly associated with the inherited disease cystic fibrosis (CF). Pseudomonas aeruginosa is well known to have a large and adaptable genome that enables it to colonise a wide range of ecological niches. Here, we have used a comparative genomics approach to identify changes that occur during infection of the CF lung.

Aqueous suspension polymerization of isobutene initiated by 1,2-C6F4[B(C6F5)2]2.

Lewis acidic, chelating diborane 1 forms stable oxonium acids 2 in the presence of excess MeOH or water. Diborane 1 is shown to be an effective co-initiator for the suspension polymerization of isobutene in aqueous media at sufficiently low temperatures. Poly(isobutene) or butyl rubber is obtained at moderate to high conversion and with Mw < 200 K and PDI approximately 2 under these conditions.

Isobutene polymerization using a chelating diborane co-initiator.

Lewis acidic diborane 1 (J. Am. Chem.