Kinetic implication of moving warm side-stream Anaerobic ammonium oxidizing bacteria to cold mainstream wastewater.

Decreased activity of Anaerobic ammonia oxidation (Anammox) at low temperatures is widely reported and one of the key challenges to applying Anammox to mainstream wastewater treatment. In this work, we systematically measured Anammox across a range of temperatures for biomass taken from a side-stream (25-30 °C) Anammox system. Using the Cardinal temperature model with inflection (CTMI) and parameters T = 10 °C, T = 31 °C, T = 40 °C, and µ = 0.

Affinity informs environmental cooperation between ammonia-oxidizing archaea (AOA) and anaerobic ammonia-oxidizing (Anammox) bacteria.

Anaerobic ammonia-oxidizing (Anammox) bacteria (AnAOB) rely on nitrite supplied by ammonia-oxidizing bacteria (AOB) and archaea (AOA). Affinities for ammonia and oxygen play a crucial role in AOA/AOB competition and their association with AnAOB. In this work we measured the affinity constants for ammonia and oxygen (half-saturation; k) of two freshwater AOA enrichments, an AOA soil isolate (N.

New directions in biological nitrogen removal and recovery from wastewater.

This review summarizes strategies for biological nitrogen removal (BNR) and recovery from wastewater. The most commonly used BNR technology nitrification/denitrification is also the most energy intensive, even though there are lower energy options, including nitritation/denitritation and more efficient partial nitritation/Anammox; the latter is well demonstrated for side-stream treatment and progressing toward mainstream applications. Nitrogen recovery can be done through cell assimilation with phototrophs, but bottlenecks with solids separation and space requirements limit applications to tertiary treatment.

Uptake of phosphate by Synechocystis sp. PCC 6803 in dark conditions: Removal driving force and modeling.

Rapid uptake of inorganic phosphate (Pi) by microalgae should occur through two processes operating in parallel: onto extracellular polymeric substances (EPS) and intracellular polymeric substances (IPS). Most previous studies focused only on overall Pi uptake and ignored the roles of EPS. We investigated the two-step removal of Pi by Synechocystis sp.

Growth kinetics and mathematical modeling of Synechocystis sp. PCC 6803 under flashing light.

In photobioreactors and natural systems, microalgae are subjected to rapidly changing light intensities (LI) due to light attenuation and mixing. A controlled way to study the effect of rapidly changing LI is to subject cultures to flashing light. In this study, series of flashing-light experiments were conducted using Synechocystis sp.

Effect of culture density on biomass production and light utilization efficiency of Synechocystis sp. PCC 6803.

The viability of large-scale microalgae cultivation depends on providing optimal growth conditions, for which a key operational parameter is culture density. Using Synechocystis sp. PCC 6803, we conducted a series of fixed-density, steady-state experiments and one batch-growth experiment to investigate the role of culture density on biomass production and light utilization efficiency.

How myristyltrimethylammonium bromide enhances biomass harvesting and pigments extraction from Synechocystis sp. PCC 6803.

Myristyltrimethylammonium bromide (MTAB) is a cationic surfactant used to improve biomass harvesting and pigment extraction form microalgae, but the mechanisms underlying its effectiveness are poorly defined. We document the mechanisms for enhanced harvesting and pigment extraction for the cyanobacterium Synechocystis sp. PCC 6803 using measurements from flow cytometer, zeta potential, release of soluble components, and microscopy.

The distribution of phosphorus and its transformations during batch growth of Synechocystis.

Phosphorus (P) is an essential nutrient that affects the growth and metabolism of microalgal biomass. Despite the obvious importance of P, the dynamics of how it is taken up and distributed in microalgae are largely undefined. In this study, we tracked the fate of P during batch growth of the cyanobacterium Synechocystis sp.