Publications by authors named "Leuvenink J"
Article Synopsis
- The study aimed to understand the immune response in patients with Legionnaires' disease by examining changes in lymphocyte subpopulations during the acute and subacute phases of the illness.
- Blood samples from patients showed a significant increase in overall lymphocyte counts from the acute phase to the subacute phase, particularly in T-cell counts, while B-cell counts remained unchanged.
- The findings indicate that while the acute phase is marked by low lymphocyte levels, there's a recovery and activation of T-cells in the subacute phase, supporting their critical role in the immune response.
Increased circulating apoptotic lymphocytes in children with Down syndrome.
Pediatr Blood Cancer
December 2012
Down syndrome (DS) resembles immunodeficiency with increased infections, auto-immune diseases, and hematological malignancies. Until now, immunological studies in DS mainly focused on T-lymphocytes. We recently described a profound B-lymphocytopenia in children with DS.
View Article and Find Full Text PDFImmunophenotyping of blood lymphocyte subpopulations is an important tool in the diagnosis of immunological and haematological diseases. Paediatric age-matched reference values have been determined for the major lymphocyte populations, but reliable reference values for the more recently described T lymphocyte subpopulations, like different types of memory T lymphocytes, recent thymic emigrants, regulatory T cells and CXCR5(+) helper T lymphocytes, are not sufficiently available yet. We determined reference values for the absolute and relative sizes of T lymphocyte subpopulations in healthy children using the lysed whole blood method, which is most often used in diagnostic procedures.
View Article and Find Full Text PDFArticle Synopsis
- Reference values for B-lymphocyte subpopulations in healthy children were determined using a more accurate statistical method called tolerance intervals, rather than traditional percentiles based on small test groups.
- Age significantly impacts TACI-expression on B-lymphocytes in children, which is crucial for understanding common variable immunodeficiency disorders (CVID).
- Current classifications for CVID, primarily developed for adults, may not be suitable for children due to the evolving nature of their immune systems, especially since significant changes in B-lymphocyte subpopulations occur before age two.
In preterm neonates the immune system is thought to be less developed at birth, but very little is known about the actual size of lymphocyte subpopulations, and even less about the maturation of these subpopulations during the first months after a premature birth. To evaluate the development of lymphocyte subpopulations in preterm infants during the first 3 months after birth, we performed a prospective longitudinal study in two hospitals in the Netherlands. Preterm neonates (n = 38) of all post-menstrual ages were included and blood samples were taken from cord blood, and at 1 week, 6 weeks, and 3 months.
View Article and Find Full Text PDFSix monoclonal antibodies raised against Escherichia coli O111 and against its rough mutant J5 (chemotype Rc) were studied. One IgG2A, one IgM anti-J5, and one IgG2A anti-O111 monoclonal antibody did not bind to lipopolysaccharides of the homologous strain, but cross-reacted with heterologous gram-negative rods in an enzyme-linked immunosorbent assay. These three monoclonal antibodies activated complement when incubated with homologous or heterologous strains, but were opsonic neither in the presence nor in the absence of complement.
View Article and Find Full Text PDF