[Growth and spore germination factors of various strains of Bacillus sphaericus].

The work was aimed at studying the requirements of sixteen Bacillus sphaericus strains with a different larvicidal activity in amino acids and some other compounds necessary for their growth and spore germination. Most of the strains were found to require arginine, glutamate, methionine, threonine, serine, glycine, alanine and lysine, but they did not assimilate phenylalanine and proline. Arginine, methionine and glutamate were shown to be the most effective inductors of spore germination.

Determination of localization of ribonuclease in Aspergillus clavatus.

Indirect fluorescent antibody method was used to demonstrate the localization of A. clavatus ribonuclease in apical cell ends. Using protoplasts, ribonuclease was found to be present mainly in the periplasmic space.

[Localization of secreted ribonuclease in the cell walls of Aspergillus clavatus].

The indirect technique of fluorescent antibodies was used to detect exocellular ribonuclease of Aspergillus clavatus. The cell walls were shown to have regions responsible for the enzyme excretion.

[Method of determining the activity of beta-galactosidase with lactose as substrate].

A method for estimating beta-galactosidase activity (beta-D-galactosidase-galactohydrolase, EC 3.2.1.

[Purification and properties of beta-galactosidase from Alternaria tenius].

beta-Galactosidase from Alternaria tenius was purified to homogeneity from the cultural fluid using acetone precipitation, ion-exchange chromatography on DEAE-cellulose, adsorption on hydroxylapatite and affinity chromatography on N-(beta-D-galactopyranosyl-thiocarbamoyl)-beta-aminocaproyl-AN-Sepharose 4B. The enzyme homogeneity was demonstrated by ultracentrifugation and polyacrylamide gel electrophoresis with SDS or without it. The specific activity of the homogeneous enzyme is 160 u.

[Optimization of the conditions for beta-galactosidase biosynthesis in eukaryotes].

Liquid whey can be subsituted by dry whey in the growth medium for Saccharomyces fragilis producing endocellular beta-galactosidase. The total biosynthesis of beta-galactosidase by the yeast on the medium containing dry whey can be increased by 40-50 percent as a result of additional stepwise introduction of lactose into the medium or optimization of the medium by mathematical planning of the experiment. Constructive metabolism of the yeast is not correlated with the rate of biosynthesis of beta-galactosidase.

[Immobolization of beta-galactosidase on silochrome].

Beta-Galactosidase (beta-D-galactoside galactohydrolase 3.2.1.

[Preparation and properties of beta-galactosidase linked covalently with KM-cellulose].

Fungal beta-galactosidase was immobilized by covalent binding with KM-cellulose. The resultant preparation contained 3 mg protein per 1 g carrier; its specific activity was 65% of the initial one. As a result of immobilization pH optimum remained unchanged whereas the temperature optimum decreased from 65 degrees to 50 degrees.

[Ion exchange purification of some enzymes on KMT carboxyl cation exchanges].

Highly purfied preparations of the enzymes--yeast beta-fructofuranosidase, fungal beta-galactosidase and bacterial proteases have been isolated from crude preparations or culture liquids by adsorption on KMT microporous carboxyl cation exchanger. During desorption the enzyme activity has fully recovered and the specific activity increased 4.5-fold for beta-galactosidase and 54-fold for proteases.