Seroconversion and dynamics of IgG anti-SARS-CoV-2 antibodies during the pandemic: A two-month observation cohort study on the population of Sleman in Indonesia.

Background: This study describes the seroconversion and serodynamics of IgG antibodies against the RBD of SARS-CoV-2 in the general population of Sleman District, Yogyakarta Special Province. We aim to identify possible factors that correlate with the seroconversion and serodynamics of IgG antibodies against the RBD of SARS-CoV-2.

Methods: We performed a longitudinal study of the population at Health and Demographic Surveillance System (HDSS) Sleman, Yogyakarta, Indonesia.

Modification of cryoprotectants on sperm cryopreservation: A study of embryo development.

Objective: This research was conducted to analyze the effect of cryopreservation of sperm using modified cryoprotectants on embryo development through fertilization (IVF). In this research, three types of cryoprotectant combinations were compared, including Nakagata, modified cryoprotectant, and commercial (Kitazato).

Materials And Methods: Several parameters, namely sperm concentration, motility, morphology, plasma membrane integrity, cryo-survival rate, and viability rate, were measured and compared before vitrification and warming.

Profiling of secondary metabolites produced by Pseudomonas sp. isolate PY-122 and PY-142 as biocontrol agent againts fusarium wilt disease on chili.

Fusarium wilt is an important disease on chili plants caused by the fungus Fusarium oxysporum. This study aims to determine the effectiveness of secondary metabolites from 2 Pseudomonad isolates in controlling Fusarium wilt in chili plants and their effect on chili plant growth. This research was conducted in two stages, namely in vitro, which was carried out in the plant health laboratory and greenhouse, Faculty of Agriculture, Universitas Pembangunan Nasional Veteran Jawa Timur, and in vivo which was carried out in chili plantations known to be endemic to Fusarium wilt in Menganti, Gresik.

Mesenchymal stem cell secretome: A promising therapeutic strategy for erectile dysfunction?

Objective: The secretome, comprising bioactive chemicals released by mesenchymal stem cells (MSCs), holds therapeutic promise in regenerative medicine. This review aimed to explore the therapeutic potential of the MSC secretome in regenerative urology, particularly for treating erectile dysfunction (ED), and to provide an overview of preclinical and clinical research on MSCs in ED treatment and subsequently to highlight the rationales, mechanisms, preclinical investigations, and therapeutic potential of the MSC secretome in this context.

Methods: The review incorporated an analysis of preclinical and clinical research involving MSCs in the treatment of ED.

Fractional exhaled nitric oxide (FeNO) among school children in Java and Sumatra, Indonesia: associations with respiratory symptoms, house dust mite sensitization and the home environment.

Objective: To study associations between fractional exhaled nitric oxide (FeNO) and asthma, airway symptoms, sensitization to common allergens, outdoor pollution and home environment among 380 students in eight junior high schools in two areas in Indonesia.

Methods: Data on health and home were collected by a face-to face interview before measuring FeNO and performing skin prick test against common allergens. Exploratory linear mixed and logistic regression models were employed.

Anti-Infection of Oral Microorganisms from Herbal Medicine of Ruiz & Pav.

The WHO Global Status Report on Oral Health 2022 reveals that oral diseases caused by infection with oral pathogenic microorganisms affect nearly 3.5 billion people worldwide. Oral health problems are caused by the presence of and in the oral cavity.

Proportion of Hypogonadism in Transfusion-Dependent Thalassemia Patients and Its Contributing Factors.

Background: Beta thalassemia is a lifelong disease involving malformed red blood cells (RBC). One of the disease's complications is hypogonadism, in which adults tend to exhibit regression in sexual characteristics, experience sexual dysfunction, and therefore have a lower quality of life. Around 3-10% of the Indonesian population carries the beta-thalassemia gene.

High P16INK4A Expression, Keratinizing Features, and Surgical Margin-Free Status are Associated with Improved Survival in Patients with Oral Squamous Cell Carcinomas.

Background: Oral squamous cell carcinoma (OSCC) continues to prevail as a highly prevalent cancer in Southeast Asia and causes a significant health burden. Stratification of patients with high risks of recurrence and mortality is important in the planning of treatment and surveillance.

Methods: Formalin-fixed paraffin-embedded (FFPE) tissues of OSCC were immuno-stained and analyzed for p16 expression.

Lessons Learned From Telephone-Based Data Collection for Health and Demographic Surveillance Systems During the COVID-19 Pandemic in Indonesia.

The Sleman Health and Demographic Surveillance System (HDSS) is a longitudinal survey held routinely since 2014 to collect demographic, social, and health changes in Sleman Regency, Special Region of Yogyakarta, Indonesia. During the COVID-19 pandemic in Indonesia, we needed to adjust our method of conducting data collection from in-person to telephone interviews. We describe the Sleman HDSS data collection strategy used and the opportunities it presented.

Volunteering and instrumental support during the first phase of the pandemic in Europe: the significance of COVID-19 exposure and stringent country's COVID-19 policy.

Background: The COVID-19 control policies might negatively impact older adults' participation in volunteer work, instrumental support provision, and the likelihood of receiving instrumental support. Studies that quantify changes in these activities and the related factors are limited. The current study aimed to examine the level of volunteering, instrumental support provision and receipt before and during the first phase of the COVID-19 pandemic in Europe and to determine whether older adults' volunteering, instrumental support provision and receipt were associated with individual exposure to COVID-19 and the stringency of country's COVID-19 control policy during the first phase of the COVID-19 pandemic.

Abundance and characterization of microplastic pollution in the wildlife reserve, Ramsar site, recreational areas, and national park in northern Jakarta and Kepulauan Seribu, Indonesia.

This is the first study to evaluate the presence and distribution of microplastics in sediments in the regions with a unique degree of complexity, such as wildlife reserve areas, a Ramsar site that connects directly to Greater Jakarta's mainland, recreational islands, and a marine national park. Microplastics of varying sizes and shapes are found in all places, with an increase trend in the abundance toward areas near to the epicenter of human activity. Comparatively to other marine protected areas, the amount of microplastics discovered is comparable; however, there is an upward trend.

Multidisciplinary Team Meeting in the Core of Nasopharyngeal Cancer Management Improved Quality of Care and Survival of Patients.

Nasopharyngeal cancer (NPC) cases are often diagnosed in advanced stages. The complexity of clinical management for advanced-stage NPC requires thorough communication and shared decisions between medical professionals and allied teams. Incorporating a multidisciplinary team meeting (MDTM) for newly diagnosed NPC patients was chosen to facilitate collaboration and communication between physicians.

Propagation of tropical squall line-induced storm coastal inundation episodes in Java-Bali, Indonesia.

The short-lived tropical squall lines could trigger weather-related hazards to the northern part of the Indonesia Maritime Continent (IMC), such as Sumatra and Kalimantan. Herein, we investigated the rare propagation event of the long-lived Sumatra squall line associated with a severe storm surge that induced coastal inundation in Java-Bali with devastating impacts from 22 May-2 June 2020. With a comprehensive approach combining observational, numerical, and analytical studies, for the first time, we proposed the possible mechanism related to the long-lived squall line over the IMC, which represents the largest equatorial tropical region with the most complicated air-sea interaction area in the world.

Impacts of COVID-19 Pandemic on Three IVF Clinics of Jakarta, Indonesia: A Retrospective Qualitative and Quantitative Study.

Background: Corona virus disease-19 (COVID-19) pandemic also led to a reduction or even the suspension of elective health services. These decisions affected fertilization (IVF) programs worldwide. Therefore, it is essential to map the readiness of IVF clinics in providing safety in this situation and in the future.

The effect of feeding with different protein levels on internal organ weight and gene expression of MEF2A and ATF3 in crossbred local chicken using RT-PCR.

Background: Myogenic enhancer transcription factor 2A (MEF2A) is a transcription factor known for its role in controlling skeletal muscle regeneration and metabolic processes, while activating transcription factor 3 (ATF3) is a stress-induced transcription factor that plays a role in modulating metabolic processes, immunity, and oncogenesis. Environmental factors, such as dietary protein, can influence gene expression levels. Insufficient protein intake can negatively affect the metabolic performance of internal organs, leading to the abnormal weight of internal organs.

Preparation of decaffeinated coffee extract: study of the effectiveness of decaffeinated coffee extract toward lowering blood sugar in type 2 diabetes mellitus patients.

Introduction: Diabetes mellitus is a disease characterized by an increase in blood sugar levels due to abnormalities in the insulin hormone system. The number of people with this disease is expected to increase every year. Therefore, it is necessary to develop diabetes mellitus drugs that have effective performance in reducing blood glucose level.

The diuretic effect of ethyl acetate fractions of Artocarpus altilis, Artocarpus champeden, and Artocarpus heterophyllus leaves in normotensive Wistar rats.

Background: Artocarpus altilis, A. Champeden, and Artocarpus heterophylus are popular species in Indonesia, and are commonly used as traditional medicine.

Objective: This study aims to evaluate the diuretic effects of the ethyl acetate fraction of these three species on normotensive Wistar rats.

Characterization and Study of Micro-encapsulation Chitosan Alginate of Single-bulb Garlic Extract.

Background: Single-bulb garlic extract (SBGE) contains more active compounds than regular garlic, but it is unstable and easily degraded in the digestive tract. SBGE is expected to be protected by microencapsulation chitosan-alginate (MCA).

Objective: The present study aimed to characterize and assess the antioxidant activity, hemocompatibility, and toxicity of MCA-SBGE in 3T3-L1 cells.

The seahorse ( L.) extract ameliorates sperm qualities, testosterone level, and serum biochemistry in rats induced by depo medroxyprogesterone acetate.

Objective: The percentage of infertility cases in this world is about 50%. Seahorses ( spp.) are widely used in traditional medicine.

Comparison of sperm parameters and DNA fragmentation index between infertile men with infection and vaccines of COVID-19.

Several preventive measures, including vaccination, have been implemented owing to the severe global effect of coronavirus disease 2019 (COVID-19), but there is still limited evidence in the effect of this disease and vaccination against it on male fertility. Therefore, this study is to compare sperm parameters of infertile patients with or without COVID-19 infection and the effect of COVID-19 vaccine types on them. Semen samples of infertile patients were collected consecutively at Universitas Indonesia - Cipto Mangunkusumo Hospital (Jakarta, Indonesia).

The Valid and Reliable Semi-Quantitative Food Frequency Questionnaire among the Sleman Under Five Children.

To examine macronutrient intake among under-five children, which is cost-effective, reliable, and validated by regional food choice and availability, we developed a new Semi Quantitative-Food Frequency Questionnaire (SQ-FFQ). We validated the Sleman Under Five Children SQ-FFQ (SUFS) compared to the 3-time 24-hour recalls (24HR) and conducted reliability tests between the first and second SUFS interviews. This study included 102 under-five children.

Identification of begomoviruses associated with the insect vector Bemisia tabaci and various host plants on Java Island, Indonesia.

Begomoviruses are economically important plant viruses and are transmitted by Bemisia tabaci which is a complex of various cryptic species. However, it is uncertain whether most begomoviruses that infect host plants are transmitted by B. tabaci at a similar rate.

Mental health literacy questionnaire-short version for adults (MHLq-SVa): validation study in China, India, Indonesia, Portugal, Thailand, and the United States.

Background: Mental Health Literacy (MHL) has become a focus of research in recent decades, as a prerequisite for early identification and intervention for mental health problems. Although several instruments have been developed for assessing MHL, there is a need for brief and psychometrically sound measures to capture important aspects of MHL in large and diverse adult samples. The present study aimed to: (1) provide a revised and shorter version of a previously validated questionnaire for assessing MHL; and (2) examine the psychometric properties of the MHLq-SVa in student samples from six different countries (China, India, Indonesia, Portugal, Thailand, and United States).

Genetic diversity of cryptic species of Bemisia tabaci in Asia.

Bemisia tabaci is a species complex consisting of various genetically different cryptic species worldwide. To understand the genetic characteristics and geographic distribution of cryptic species of B. tabaci in Asia, we conducted an extensive collection of B.

Two-years antibody responses following SARS-CoV-2 infection in humans: A study protocol.

The long-term antibody response to the novel SARS-CoV-2 in infected patients and their residential neighborhood remains unknown in Indonesia. This information will provide insights into the antibody kinetics over a relatively long period as well as transmission risk factors in the community. We aim to prospectively observe and determine the kinetics of the anti-SARS-CoV-2 antibody for 2 years after infection in relation to disease severity and to determine the risk and protective factors of SARS CoV-2 infections in the community.