Publications by authors named "Leslie Rodriguez-Kirby"
Article Synopsis
- * The researchers developed a new method called TRISCO, which helps retain RNA signals in cleared organs for better imaging, specifically for whole-brain 3D RNA visualization.
- * TRISCO effectively preserves RNA integrity, ensures uniform labeling, and enhances tissue transparency, making it a powerful tool for detailed transcriptional spatial analysis at the single-cell level across various organs.
The ability to spatially map multiple layers of the omics information over different time points allows for exploring the mechanisms driving brain development, differentiation, arealization, and alterations in disease. Herein we developed and applied spatial tri-omic sequencing technologies, DBiT ARP-seq (spatial ATAC-RNA-Protein-seq) and DBiT CTRP-seq (spatial CUT&Tag-RNA-Protein-seq) together with multiplexed immunofluorescence imaging (CODEX) to map spatial dynamic remodeling in brain development and neuroinflammation. A spatiotemporal tri-omic atlas of the mouse brain was obtained at different stages from postnatal day P0 to P21, and compared to the regions of interest in the human developing brains.
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