Publications by authors named "Leslie Aranda"
In the present study we sought to improve the efficacy and safety of our Sca1 PDGFB stem cell gene therapy for osteoporosis in ovariectomized (OVX) mouse model. This therapy is administered by marrow transplantation. We established the promise of this approach by previously showing that this therapy in normal mice increase bone density, increased endosteal cortical and trabecular bone formation, caused de novo trabecular bone formation, increased cortical thickness and improve bone strength.
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- - Genome editing of human induced pluripotent stem cells (iPSCs) is crucial for research in genetics, disease studies, and regenerative medicine, but low efficiency in CRISPR-Cas9 methods has been a barrier due to high cell death after electroporation.
- - Introducing BCL-XL significantly boosts iPSC survival and enhances genome editing success, with increases of 20- to 100-fold in gene insertion efficiency and up to 5-fold in gene deletion success.
- - Further use of the BCL inhibitor ABT-263 improves editing efficiency even more, enabling near-perfect knockout rates and making extensive genetic modifications in cell populations easier without the need for time-consuming individual cell cloning.
A sensitive and dynamically responsive auxin signaling reporter based on the DII domain of the INDOLE-3-ACETIC ACID28 (IAA28, DII) protein from Arabidopsis (Arabidopsis thaliana) was modified for use in maize (Zea mays). The DII domain was fused to a yellow fluorescent protein and a nuclear localization sequence to simplify quantitative nuclear fluorescence signal. DII degradation dynamics provide an estimate of input signal into the auxin signaling pathway that is influenced by both auxin accumulation and F-box coreceptor concentration.
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