[Malignant dermoid cyst of the ovary with double tumor cell population, revealed by an umbilical metastasis].

The authors report a case of multiple malignancies (squamous cell carcinoma and carcinoid) arising in a dermoid cyst of the ovary. The tumor being revealed by an umbilical metastasis. They insist on the rarity of this entity and discuss the elements of diagnosis and prognosis through a review of literature.

Adhesive properties of human erythroblastic precursor cells.

Interactions between hematopoietic precursor cells and their surrounding marrow environment are essential for hematopoietic differentiation. These occur in part through the production of regulatory molecules by marrow stromal cells and their local concentration by components of the extracellular matrix (ECM), but direct cell-cell or cell-matrix contacts are likely to also play an essential role. During the past several years, we have characterized the adhesive properties of human hematopoietic precursor cells on two substrates, marrow fibroblastic ECM and purified plasma fibronectin (Fn).

Coexpression of two fibronectin receptors, VLA-4 and VLA-5, by immature human erythroblastic precursor cells.

Human erythroblastic precursor cells adhere to fibronectin (Fn) but the exact nature of the receptors mediating this interaction has not been characterized. In this study, we report data showing that immature human erythroblasts express the integrins VLA-4 and VLA-5 and that both these molecules act as fibronectin receptors on these cells. We have recently demonstrated that adhesion to Fn of purified human CFU-E and their immediate progeny preproerythroblasts was inhibited by antibodies directed against the human fibronectin receptor (VLA-5).

[Arterial macro-aneurysm].

Retinal macroaneurysms cause visual symptoms either by hemorrhage, by exsudation or by arterial occlusion. Laser treatment shortens life duration of the macro-aneurysm and results in reabsorption of retinal edema. Direct coagulation of the macroaneurysm should be avoided so as not to enhance the risk of arterial occlusion.

Loss of attachment to fibronectin with terminal human erythroid differentiation.

Human erythroblastic progenitors (colony-forming unit-erythroid [CFU-E] and burst-forming unit-erythroid [BFU-E]) have been shown to attach to fibronectin (Fn), a property that might be involved in the local regulation of erythropoiesis. In this study, we have investigated changes in cell attachment to Fn upon terminal erythroid differentiation. We first purified CFU-E from human marrow by avidin-biotin immune rosetting.

[Primary open-angle glaucoma. Effects of an eyedrop combining timolol and pilocarpine on the ocular pressure].

In a limited group of open angle glaucoma patients treated twice daily with timoptol 0.5% and having by this treatment a mean intra-ocular pressure of 21-22 mmHg, the association of timoptol 0.5% with pilocarpine 2% respectively, lowered the intra-ocular pressure to an average of 17.

An automated micromethod for detection of lytic antimycobacterial antibodies by immune lysis of liposomes sensitized to tuberculin.

Calcein was concentrated inside multilamellar liposomes (MLV) by encapsulation through an osmotic gradient in order to increase its quenching. Immune lysis of these MLV sensitized to PPD by direct encapsulation or by exposure to preformed vesicles was studied in the presence of rabbit anti-BCG serum and of a non-immune rabbit serum as a source of complement, successively added in the wells of a microplate to a final volume of 0.1 ml.

[Atypical heart transplantation. Technical considerations. Apropos of a case].

A heterotopic heart transplantation was performed on a patient placed on circulatory assistance with a Biomedicus pump for ten days using a heart taken from a donor with situs inversus. In relation to this case, the authors describe the technical procedures allowing transplantation of a heart obtained from a donor with situs inversus into a heterotopic position and an orthotopic position or a normal heart in a recipient with situs inversus. Lastly, in the case of complex cardiac malformations with preservation of the lungs, they present the procedures allowing heart transplantation in cases of situs incertus, anomalies of venous return and transposition of the great vessels.

Granulopoietic differentiation in long-term bone marrow cultures from children with congenital neutropenia.

The capacity of granulopoietic precursor cells (CFU-GM) to differentiate in vitro was evaluated in five children with congenital neutropenia using short-term colony assays and long-term marrow cultures. In all five children, methylcellulose assays revealed normal numbers of CFU-GM, which displayed an appropriate response to various sources of GM-CSF and differentiated up to the polymorphonuclear leukocyte state (PMN). In contrast, neutrophil PMN were not observed in long-term bone marrow cultures from three patients, despite a normal production of CFU-GM, myeloblasts, and promyelocytes during the 5-6 week culture period.

Lineage- and stage-specific adhesion of human hematopoietic progenitor cells to extracellular matrices from marrow fibroblasts.

Local regulation of hematopoietic differentiation in the marrow requires close interactions with components of the microenvironment. In this study, we explored the capacity of human marrow hematopoietic progenitor cells to adhere in vitro to the extracellular matrix (ECM) secreted by human marrow fibroblasts. When marrow mononuclear cells were incubated on ECM-coated dishes, all types of progenitors adhered to this substrate through an active process requiring divalent cations and serum factors.

Comparison of two special infant formulas designed for the treatment of protracted diarrhea.

The nutritional management of intractable diarrhea and short bowel syndrome remains a challenging problem. The advantage of continuous nasogastric infusion is undisputed, but what to feed remains in question, and no studies, to these authors' knowledge, have yet compared two widely used specially designed protein hydrolysate formulas. A randomized crossover trial of two periods of 7 days was carried out with Alfare and Pregestimil administered by a constant infusion pump in six malnourished infants aged 1-13 months.

Circulating activities during constant infusion of heparin or a low molecular weight derivative (enoxaparine): failure to demonstrate any circadian variations.

We compared in six patients successively treated with an unfractionated heparin (UFH) and a low molecular weight heparin (LMWH) the variations in plasma anti-Xa activity, measured in a chromogenic assay, during a 36 h constant infusion. The values varied in a wider range during UHF infusion, but remained in the therapeutic range except once in one patient. No circadian rhythm could be demonstrated in our six patients.

Taurine improves the absorption of a fat meal in patients with cystic fibrosis.

The effect of taurine supplementation on the absorption of a fat meal was evaluated in patients with cystic fibrosis. In a cross-over design study, five patients with cystic fibrosis (12.1 +/- 2.

[When the psychiatric hospital "sleeps"...].

This paper is the conclusion of a study on nursing's transactions during the night, in a mental hospital. It is demonstrated that therapeutical premises and nursing attitudes are defined by the position of the nurse into the institutional structure. It is also demonstrated that medical prescription doesn't depend exclusively of clinical criteria but it also cares for keeping institutional order.

Taurine supplementation of a premature formula improves fat absorption in preterm infants.

The predominance of taurine (Tau) conjugated over glycine conjugated bile acids in infants fed human milk as opposed to those on formulas without added Tau could account for a more complete absorption of fat. Fifteen low birth weight infants were randomized to either Enfamil Premature or to Enfamil Premature added with 40 mumol/dl of Tau and compared to a third group made up of nine low birth weight infants fed their own mother's preterm milk. Formulas and human milk were fed according to tolerance and constituted the sole nutrition for 3 months.

[Sleep disorders in patients with personality disorders].

63 patients with personality disorders have been hospitalized in a Sleep Disorder Clinic. Sleep disorders usually started during childhood, disappeared ant the reappeared around the age of 25. The polysomnographic data do not show a specific profile.

[The microenvironment and hematopoiesis].

Successive steps in hematopoietic differentiation occur in the bone marrow in close contact with non hematopoietic components of the microenvironment. The crucial importance of these interactions in the regulation of early hematopoietic events has been convincingly demonstrated in vivo in the mutated steel mouse, and confirmed more recently in vitro in long-term cultures. Parts of the signal mediating these interactions are represented by locally secreted CSF-like growth factors or more recently characterized inhibitors of proliferation.

Effect of dietary fat and residues on fecal loss of sterols and on their microbial degradation in cystic fibrosis.

Although various etiologic factors have been implicated, the mechanism responsible for bile acid malabsorption in CF remains unknown. Eight CF children studied twice on a normal diet supplemented with pancreatic enzymes and once during a one-month period of Vivonex administered by continuous nasogastric infusion were compared to age-matched controls. On the fat and residue-free elemental diet, there was a modest decrease in steatorrhea and no change in the daily excretion of nitrogen and neutral sterols.