Exceptional Extension of Benign Angiomyolipoma in the Renal Vein.

When a renal angiomyolipoma (AML) is incidentally detected on imaging, the venous system should be assessed for intravascular fat component.

Relevance of the quantitative detection of HIV proviral sequences in PBMC of infected individuals.

Changes in HIV replication during progression of HIV infection were assessed by estimation of the number of peripheral blood mononuclear cells (PBMC) harboring HIV proviral sequences. Samples from 23 patients at different stages of HIV infection were analyzed by quantitative polymerase chain reaction (PCR) using GAG primers. HIV titers in PBMC were also determined by serial dilutions of cells in coculture with phytohemagglutinin-activated normal PBMC.

Phytohemagglutinin and concanavalin A activate hepatitis B virus in peripheral blood mononuclear cells of patients with chronic hepatitis B virus infection.

Peripheral blood mononuclear cells (PBMC) from 25 patients with chronic hepatitis B were tested for the presence of free monomeric hepatitis B virus (HBV) DNA migrating as a single 3.2 Kb band by Southern blot analysis. The PBMC were cultured for 7 days in the presence of phytohemagglutinin (PHA) or concanavalin A (ConA) both of which yielded a proliferative response.

Immunoquantitation of von Willebrand factor (factor VIII-related antigen) in vinyl chloride exposed workers.

Angiosarcoma of the liver is a rare malignant tumor which has been associated with occupational exposure to vinyl chloride (VC). We have determined by ELISA the level of von Willebrand factor (vWf) in the serums of 107 VC-exposed workers, active or retired, and of 133 blood donors used as controls. The vWf level was slightly but significantly higher in the VC-exposed group than in the control group (P = 0.

HIV detection in culture of peripheral blood mononuclear cells: a relevant in vitro correlate of the antiviral effect of ZDV in vivo.

To better assess the antiviral effect of zidovudine (ZDV) in vivo and understand its limitations, we have studied human immunodeficiency virus (HIV) replication in peripheral blood mononuclear cell (PBMC) cultures from 25 ZDV-treated patients and 20 untreated controls. Three months after initiation of therapy, 9 of the 25 treated cases became negative for HIV isolation (36%). Untreated cases never converted to a negative culture.

Generation of a 1.5-kb cDNA fragment of the hepatitis C virus genome by overlap extension.

Hepatitis C virus (HCV) is a recently identified RNA virus responsible for most of non-A, non-B hepatitis. Genetic analysis of HCV sequences and their encoded proteins has been hampered by the difficulty in obtaining cDNA fragments of sufficient lengths: construction of cDNA library requires technical expertise while amplification by the polymerase chain reaction (PCR) usually yields fragments of less than 400 base pairs. In this report we have generated a 1.

Plasma viraemia as a marker of viral replication in HIV-infected individuals.

Two hundred and twenty-eight blood samples from 154 HIV-seropositive subjects were investigated for the presence of HIV in peripheral blood mononuclear cells (PBMC) and plasma (without prior ultracentrifugation or filtration), using normal PBMC as the target for replication. HIV was recovered from PBMC and plasma in 80.5 and 19.

Two French genotypes of hepatitis C virus: homology of the predominant genotype with the prototype American strain.

A hepatitis C virus (HCV) cDNA covering part of the nonstructural region, NS3, was amplified from the serum of 50 out of 76 French non-A, non-B hepatitis patients by the nested polymerase chain reaction (PCR). Determination of a 407-bp sequence from four such cases revealed the presence of two different virus genotypes, F1 and F2, which exhibited 19-20% sequence divergence. F1 was represented by three of the four isolates and showed a sequence homology of about 97.

Evidence of two major genotypes of hepatitis C virus in France and close relatedness of the predominant one with the prototype virus.

Hepatitis C virus (HCV) cDNA sequence in the nonstructural region NS3 was amplified from the serum of 66% French non-A, non-B hepatitis patients by the nested polymerase chain reaction. A 407 base-pair sequence was determined from four such cases, which revealed the presence of two different virus genotypes F1 (three cases) and F2 (one case) with 19-20% sequence divergence. F1 showed close homology (97.

Effect of manganese ions on the immune response of carp (Cyprinus carpio) against Yersinia ruckeri.

The effect of manganese ions Mn2+ (Mn Cl2) on hematocrit and the antibody response of carp (Cyprinus carpio) was investigated after exposure of fish to contaminated water for 2.5 months. Contamination of water with 50 mg/l Mn2+ did not decreased antibody production against Yersinia ruckeri (measured by agglutination) but significantly decreased hematocrit.

Effect of gamma-hexachlorocyclohexane (lindane) on carp (Cyprinus carpio). II. Effects of chronic intoxication on blood, liver enzymes, and muscle plasmic membrane.

A 30-day ingestion of gamma-hexachlorocyclohexane (lindane) by carp (Cyprinus carpio) induced hypoglycemia without activation of two hepatic gluconeogenesis enzymes (fructose diphosphatase, EC 4.1.2.

Effect of gamma-hexachlorocyclohexane (lindane) on carp (Cyprinus carpio). I. Effect of chronic intoxication on humoral immunity in relation to tissue pollutant levels.

Lindane, the gamma-isomer of hexachlorocyclohexane, one of the most widely used insecticides, was incorporated into carp food for 109 days. The effects of 10, 100, or 1000 ppm of lindane in food on hematocrits and on antibody production against Yersinia ruckeri bacterin in sera and mucus were followed for 79 days. No effect of lindane on these parameters and on spleen weight was observed.

[Intestinal adaptation and enzymatic changes following reciprocal jejunoileal transposition in rats. Effects of a high-fructose diet].

The longitudinal localization of nine enzymes of the carbohydrate metabolism was studied in rats fed standard or high fructose diets, two months after a reciprocal jejuno-ileal transposition. In the ileal segment transposed to jejunal location, an adaptive increase of mucosal mass was observed, but the functional characteristics of enterocytes remained the same in the case of triokinase, aldolase, triose phosphate isomerase, glucose-6-phosphate isomerase and glucose-6-phosphatase activities. In the case of ketohexokinase and hexokinase activities, the functional properties of cells tended to resemble that of jejunum, as revealed by a significant increase in the specific enzyme activity.

In vitro toxicity of halogenated anaesthetics on guineapig alveolar macrophages, surviving in gas phase.

The potential toxicity of halogenated anaesthetics on cellular immunity has been investigated on guineapig macrophages in gas phase by measuring the adenosine 5' triphosphate (ATP) concentration in the macrophages. A toxicity index (TI) was obtained by comparing the ATP concentration in vapour or gas-exposed macrophages with that of control cells. The TI for 5% halothane in air after 24 h was 31.