[Change in the production of key cytokines for regulation eosinophilic granulocytes in Opisthorchis felineus invasion].

Opisthorchis invasion is accompanied by the imbalanced lymphocytic subpopulational composition manifested itself as induction of the B-link and, on the contrary, depression of T-lymphocytic populations (CD4+, CD8+), with their weaker helper-suppressor association. The immunocompetent cells were ascertained to show a higher production of TH2 cytokines that had an eosinophil-stumulating effect.

[Opisthorchis felineus (Rivolta, 1884) and Metorchis bilis (Braun, 1890) infections in population of some regions of the Ob River Basin].

The incidence of Opisthorchis felineus (Rivolta, 1884) and Metorchis bilis (Braun, 1890) infections among people living in several regions of the Ob River basin in the West Siberia has been assesed in this work. Our results suggest that Metorchis bilis infection was common in many of the serologically tested people. Moreover, this helminth was obtained from the biliary ducts of humans in autopsy.

[Accumulation of some toxic microelements in liver tissue and helminthes obtained from patients with Opisthorhis felineus (Rivolta, 1884) and Metorchis bilis (Braun, 1890) invasion].

According to some available reports, it is known that there may be an increased accumulation of some micronutrients in the samples of hair and blood. The purpose of the present investigation was to study the general regularities of accumulation of micronutrients in the samples of liver tissue and helminthic bodies obtained from patients with opisthorchiasis who lived in Tomsk and who had different etiological types of Opisthorchis (O. felineus) and Metorchis (M.

[Dysregulation of cytokine production by mononuclear cells of peripheral blood in persistent infection with a virus of tick-borne encephalitis].

Tick-borne encephalitis is accompanied by dysregulation of cytokine production of mononuclear leucocytes of peripheral blood. The changes of production of IFN-r by mononuclear leucocytes of peripheral blood positively correlated with a degree of activity of pathological process and intensity of clinical symptoms of neuroninfection. On the contrary, secretion of IL-12 mononuclears directly correlated with virus persistence in the organism and does not depend on clinical presentation of the disease.

[Immunopathogenetic features of tick-borne encephalitis under long-term viral antigenemia].

The occurrence of chronic tick-borne encephalitis viral antigenemia characterized by the asymptomatic course or minimal clinical manifestations is caused by the virus-immune cell interaction. Cellular immunity was studied in 183 patients with chronic (more than 6-month) tick-borne encephalitis viral antigenemia, by evaluating the immunophenotypic and cytogenetic statuses, structural and functional features, cytokine profile, and peripheral lymphocytic apoptosis. The findings suggest impairments in the cooperation of immunocompetent cells and in the classical scheme of antiviral immunity regulation in tick-borne encephalitis viral persistence due to the changes in lymphocytic structural and functional properties.

[Functional status of peripheral blood monocyte in patients with Ixodes tick-borne borreliosis accompanied by opisthorchiasis].

The characteristics of the functional status of peripheral blood monocytes/macrophages in patients with Ixodes tick-borne acute borreliosis accompanied by opisthorchiasis invasion were studied. The study revealed a decrease in the phagocytic activity of monocytes and in the level of expression of cell receptors Fcgamma with the expression of cell receptors C3beta being normal.

Chronic tick-borne encephalitis virus antigenemia: possible pathogenesis pathways.

Study of the proliferative potential and immunophenotype of lymphocytes and cytokine-producing capacity of mononuclear cells in patients with chronic tick-borne encephalitis virus antigenemia showed changes in T cell lymphoproliferative response, relative content of T cells and T helper inductors, immunoregulatory index, and imbalance in the production of immunoregulatory Th1 and Th2 cytokines.

Fluorescent probing of lymphocyte and erythrocyte plasma membranes in persistence of tick-borne encephalitis virus: monitoring of structural changes.

The structure of lymphocyte and erythrocyte plasma membrane in patients with long persistence of tick-borne encephalitis virus was studied using fluorescent probes pyrene and 1-anilinonaphthalene-8-sulfonate. The authors analyze the clear-cut disorders in the membranes of both lymphocytes and erythrocytes in viral tick-borne-encephalitis from the viewpoint of generalized involvement of cell membranes in infectious process.

Structural and functional characteristics of lymphocytes in chronic carriers of tick-borne encephalitis virus.

Study of the surface phenotype, structure of plasma membrane, and activity of DNA repair system in peripheral blood lymphocytes from patients with different clinical forms of tick-borne encephalitis associated with long persistence of the virus revealed a pronounced imbalance between lymphocyte subpopulations, increased microviscosity of the plasma membrane lipid phase, and decreased activity of the DNA repair system in lymphocytes.

[Cytogenetic defects in lymphocytes of patients with chronic opisthorchiasis concurrent with Epstein-Barr virus persistence].

Sixty three patients with chronic opisthorchiasis were cytogenetically, serologically, and biochemically studied. Most patients with opisthorchiasis were found to have higher or high titers of antibodies to Epstein-Barr viral antigens. There was a direct correlation between the titers of antibodies to Epstein-Barr virus capsid antigen and the count of cytogenetically damaged cells.

[Enzyme immunoassay in diagnosis of opisthorchiasis. Communication 2. Enzyme immunoassay test system for diagnosis of acute opisthorchiasis].

A standard, highly specific and sensitive enzyme immunoassay test system has been developed for the diagnosis of acute opisthorchiasis, which is based on indirect solid-phase enzyme immunoassay for determination IgM antibodies to the antigen derived from Opisthorchis felineus marita extracts. The sensitivity of the test system is 87%, its specificity is 96.8%.

[Iodoantipyrine--an agent for treating and preventing tick-borne encephalitis].

The antiviral activity of iodantipyrine was studied in outbred [correction of inbred] albino mice (weight 10-12 g) infected with the Absettarov strain of the tick-borne encephalitis virus. Iodantipyrine was administered per os or parenterally and the animals were observed for 21 days. A reliable therapeutic effect was produced in 60% of mice infected with 10 DL50 of tick-borne encephalitis virus which were given the drug per os in a dose of 50 mg/kg.

[Immunoenzyme analysis in the diagnosis of opisthorchiasis. I. The development of an immunoenzyme method for determining IgM antibodies to the Opisthorchis antigen].

An assay was developed to determine IgM antibodies to Opisthorchis felineus antigens for the diagnosis of acute infection. The method is based on indirect solid-phase enzyme immunoassay. Optimal conditions were elaborated for analysis and its high specificity and specifications are shown in the paper.

[The vital activities of enterobacteria in an aqueous medium in the absence of sources of organic nutrition].

Heterotrophic bacteria were found to be capable of proliferation in physiological saline and distilled water. In 1988-1989 experimental studies were made with a view to establish the role of the gaseous phase of atmospheric air and the products of the autolysis of dead bacteria as the sources of organic nutrition. The studies revealed that the complete removal of atmospheric air from vials with bacterial suspension completely stopped the stimulation of reproduction.

[The function of the hypophyseal-thyroid system in chronic opisthorchiasis and its combination with the Salmonella typhi carrier state].

The hormonal function of the hypophysis and thyroid (TTG, T3, T4) and its dependence on hepatobiliary function, examined by hepatobiliscintigraphy, were studied by radioimmunoassay in 71 patients with chronic opisthorchiasis and in 18 chronic carriers of Salmonella typhi. in both groups. The decrease of T3 and T4 in the presence of normal TTG level was found.

[The effect of the anthelmintics biltricide and chloxyl on the level of cellular and humoral immunity in patients with chronic opisthorchiasis].

The effect of two anthelmintics--biltricide and chloxyl--on the immune status has been compared in patients with chronic opisthorchiasis. It has been established that in using chloxyl there is a more marked and persistent imbalance of immunoregulatory T-lymphocyte subpopulations (T mu-helpers-inducers and T gamma-suppressors-effectors).

The analysis of some indices of immune response, DNA repair, and micronuclei content in cells from tick-borne encephalitis patients.

Patients with tick-borne encephalitis (TBE) had higher counts of red blood cells (RBC) with micronuclei. The majority of patients revealed decreased capacity of blood lymphoid cells for DNA repair except those with a 2-wave pattern of the course of disease; in the latter, the DNA repair was significantly higher than in healthy donors. Patients with TBE revealed lower T-lymphocyte counts due to a decrease in the amount of T-helper cells (the level of T-suppressors was elevated).

[The insulin, C-peptide and glucagon content of the blood of patients with chronic opisthorchiasis].

Insulin, C-peptide and glucagon levels were measured by radioimmunoassay in the blood of 42 patients with chronic opisthorchiasis and 27 healthy subjects. An insignificant decrease in insulin levels and a drastic decrease in C-peptide and glucagon levels have been established.

[Cross-reacting antigens of Opisthorchis felineus and Salmonella typhi and their possible effect on the clinical course of typhoid fever].

The experiments on rabbits immunized with a complex O. felineus antigen and spirit abdominal typhoid vaccine enriched with Vi-antigen, as well as studies on patients with chronic opisthorchiasis and bacterial carriers of S. typhi have established the presence of cross-reacting antigens in abdominal typhoid and opisthorchiasis pathogens.

[The immune status of patients with tick-borne encephalitis studied by using an immune reagent kit for the determination of human lymphocyte subpopulations].

The comparative study of disturbances in the immune status of patients with different clinical forms of tick-borne encephalitis has revealed that in the severe (meningeal) form of the disease more pronounced imbalance in the immune system develops, especially with respect to immunoregulatory subpopulations of T-lymphocytes. This seems to be the cause of delayed activation of immune processes in this group of patients in comparison to those having an aborted course of the disease.

[The effect of various factors on the immune status of patients with chronic opisthorchiasis. 2. Changes in immunologic indices in patients with chronic opisthorchiasis undergoing chloxyl treatment for various lengths of time].

Most immunological characteristics became practically normal 7-9 months after a single chloxyl treatment course in patients with chronic cholecystocholangitic opisthorchiasis. The patients who required repeated chemotherapy showed more profound and steady immune disturbances.

[The hypophyseo-thyroid system in psoriatic arthritis combined with chronic opisthorchiasis].

Paper presents the data on the content of hypophysial-thyroid and hypophysial-adrenal systems' hormones obtained by means of radioimmune assay, and the data of timecourse hepatoscintibiligraphy with Tc 99 m TCK-15 of 94 patients (first group--48 patients with psoriatic arthiritis combined with chronic opisthorchiasis; second group--46 pure psoriatic arthritis patients). Statistically significant increase in the content of triiodinethyronine, thyroxine, hypophysial thyreotropic hormone, corticotropic hormone and significant reduction of cortizol and aldosteron contents were observed in case of mixed pathology. Thyroid hormones' level was directly connected with hepatic and gallbladder disfunctions.

[The effect of Opisthorchis invasion on the clinical course and outcome of abdominal typhoid. Indicators of non-specific resistance and phagocytosis in patients with abdominal typhoid combined with chronic opisthorchiasis].

A number of non-specific resistance indices (complement, properdin, lysozyme, bactericidal activity of blood serum, phagocytosis indices) in Opisthorchis-invaded abdominal typhoid patients as well as in acute and chronic carriers of Salmonella typhi was inferior to those of uninvaded patients (with the exception of bactericidal index). Complete recovery from opisthorchiasis stimulated the cessation of S. typhi elimination and increase in the level of some non-specific resistance factors.

[Diagnostic significance of determining antibodies to antigens of the Epstein-Barr virus in infectious mononucleosis].

Infectious mononucleosis may be differentiated from clinically similar diseases by the detection of classes M and A antibodies to Epstein-Barr virus capsid antigen. The authors emphasize the high specificity of this test vs. the detection of the Paul-Bunnell-Davidson heterophilic antibodies.