Identification of organic contaminants in water and related matrices using untargeted liquid chromatography high-resolution mass spectrometry screening with MS/MS libraries.

Nontargeted and suspect screening with liquid chromatography-high resolution mass spectrometry (LC-HRMS) has become an indispensable tool for quality assessment in the aquatic environment - complementary to targeted analysis of organic (micro)contaminants. An LC-HRMS method is presented, suitable for the analysis of a wide variety of water related matrices: surface water, groundwater, wastewater, sediment and sludge, including extracts from passive samplers and on-site solid phase enrichment, while focusing on the data processing aspect of the method. A field study is included to demonstrate the practical application and versatility of the whole process.

Genetic Diversity of the Human Adenovirus C Isolated from Hospitalized Children in Russia (2019-2022).

The human adenovirus (HAdV) is a common pathogen in children that can cause acute respiratory virus infection (ARVI). However, the molecular epidemiological and clinical information relating to HAdV among hospitalized children with ARVI is rarely reported in Russia. A 4-year longitudinal (2019-2022) study among hospitalized children (0-17 years old) with ARVI in Novosibirsk, Russia, was conducted to evaluate the epidemiological and molecular characteristics of HAdV.

Examining the applicability of polar organic chemical integrative sampler for long-term monitoring of groundwater contamination caused by currently used pesticides.

The possibilities of expanding a groundwater quality monitoring scheme by passive sampling using polar organic chemical integrative sampler (POCIS) comprising HLB sorbent as the receiving phase were explored. Passive sampling and grab sampling were carried out simultaneously in the regions with vulnerable groundwater resources in Slovakia, between 2013 and 2021. For 27 pesticides and degradation products detected both in POCIS and the grab samples, in situ sampling rates were calculated and statistically evaluated.

Global air pollution exposure and poverty.

Air pollution is one of the leading causes of health complications and mortality worldwide, especially affecting lower-income groups, who tend to be more exposed and vulnerable. This study documents the relationship between ambient air pollution exposure and poverty in 211 countries and territories. Using the World Health Organization's (WHO) 2021 revised fine particulate matter (PM2.

Changes in the Etiology of Acute Respiratory Infections among Children in Novosibirsk, Russia, between 2019 and 2022: The Impact of the SARS-CoV-2 Virus.

A wide range of human respiratory viruses are known that may cause acute respiratory infections (ARIs), such as influenza A and B viruses (HIFV), respiratory syncytial virus (HRSV), coronavirus (HCoV), parainfluenza virus (HPIV), metapneumovirus (HMPV), rhinovirus (HRV), adenovirus (HAdV), bocavirus (HBoV), and others. The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the COronaVIrus Disease (COVID) that lead to pandemic in 2019 and significantly impacted on the circulation of ARIs. The aim of this study was to analyze the changes in the epidemic patterns of common respiratory viruses among children and adolescents hospitalized with ARIs in hospitals in Novosibirsk, Russia, from November 2019 to April 2022.

Measuring accessibility to public services and infrastructure criticality for disasters risk management.

Component criticality analysis of infrastructure systems has traditionally focused on physical networks rather than infrastructure services. As an example, a key objective of transport infrastructure is to ensure mobility and resilient access to public services, including for the population, service providers, and associated supply chains. We introduce a new user-centric measure for estimating infrastructure criticality and urban accessibility to critical public services - particularly healthcare facilities without loss of generality - and the effects of disaster-induced infrastructure disruptions.

Maintenance of response to oral octreotide compared with injectable somatostatin receptor ligands in patients with acromegaly: a phase 3, multicentre, randomised controlled trial.

Background: Despite biochemically responding to injectable somatostatin receptor ligands (iSRLs), many patients with acromegaly experience treatment burdens. We aimed to assess maintenance of biochemical response and symptomatic control with oral octreotide capsules versus iSRLs in patients with acromegaly who previously tolerated and responded to both.

Methods: This global, open-label, randomised controlled phase 3 trial was done in 29 clinical sites in Austria, France, Germany, Hungary, Italy, Lithuania, Russia, Serbia, Spain, and the USA.

Low incidence of human coronavirus among hospitalized children in Novosibirsk city, Russia during pre-pandemic period (2013-2020).

We investigated the incidence of 15 respiratory viruses among 2991 children with acute respiratory infections in Novosibirsk city, Russia, prior to the COVID-19 pandemic (2013-2020). Viral infections were detected in 72.5% cases.

Viral etiology of acute respiratory infections in hospitalized children in Novosibirsk City, Russia (2013 - 2017).

Background: Acute respiratory infections (ARIs) cause a considerable morbidity and mortality worldwide especially in children. However, there are few studies of the etiological structure of ARIs in Russia. In this work, we analyzed the etiology of ARIs in children (0-15 years old) admitted to Novosibirsk Children's Municipal Clinical Hospital in 2013-2017.

Symbiotic Potential of Bradyrhizobium japonicum Strains with Different Growth Rates.

Aim: To study genetic properties of soybean nodule bacteria with different growth rates, to research the ability of rhizobia to produce phytohormonal substances and estimate their symbiotic potential in the conditions of combined inoculation and mono-inoculation of soybean plants.

Methods: Sequencing of the 16S-23S rDNA intergenic spacer of soybean rhizobia was performed on ABI 3130xl Genetic Analyzer. Determination of the qualitative and quantitative composition of phytohormones in culture liquid of Bradyrhizobium japonicum strains was performed by SDTLC-chromatography of high resolution.

INFLUENCE OF BACILLUS AMYLOLIQUEFACIENS SUBSP. PLANTARUM IMV B-7404 STRAIN EXOMETABOLITES ON PHENYLALANINE AMMONIA-LYASE ACTIVITY IN WINTER WHEAT SEEDLINGS.

Influence of Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 strain exometabolites on phenylalanine ammonia-lyase (PAL, EC 4.3.

[SYNTHESIS OF PHYTOHORMONES BY NOCARDIA VACCINII IMV B-7405--PRODUCER OF SURFACTANTS].

Aim: To study the synthesis of phytohormones (auxins, cytokinins, abscisic acid) under cultivation of Nocardia vaccinii IMV B-7405 (surfactants producer) in media containing different carbon sources (glycerol, refined sunflower oil, as well as waste oil after frying potatoes and meat).

Methods: Phytohormones were extracted from supernatants of culture liquid (before or after surfactant separation) by ethylacetate (auxins, abscisic acid) and n-butanol (cytokinins), concentrated and purified by thin-layer chromatography, then quantitative determination was performed using a scanning Sorbfil spectrodensitometer.

Results: While growing in medium with refined oil IMV B-7405 strain synthesized 1.

[AUXINS AND CYTOKININES SYNTHESIS BY BRADYRHIZOBIUM JAPONICUM UNDER FLAVONOIDS INFLUENCE].

Aim: Research the ability of different by effectiveness symbiotic nitrogen-fixing soybean bacteria Bradyrhizobium japonicum to the synthesis of phytohormones-stimulators auxins and cytokinins for the actions of plant flavonoids genistein and naringenin.

Methods: Extracellular phytohormonal compound isolated from the supernatant culture liquid of the soybean rhizobia by redistribution of phytohormones in two phases solvent immiscible with each other. Auxins and cytokinins were determined by thin layer spectra densitometry chromatography.

[Antagonistic activity of Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 and BIM B-439D strains towards pathogenic bacteria and micromycetes].

In this study the antagonistic activity of strains Bacillus amyloliquefaciens subsp. plantarum IMV B-7404 and BIM B-439D against bacterial and fungal pathogens of agricultural crops has been researched. It is shown that both strains of bacilli demonstrated a high level of antagonism to the vascular bacteriosis pathogen, average level of antagonism to micromycetes--root rot pathogens.

[Group enterovirus infection due to coxsackievirus A16 in Northwestern Russia].

Aim: Study features of epidemic process and etiology of oral cavity and limb enterovirus exanthema group diseases in a number of territories of Northwestern Russia.

Materials And Methods: Isolation and identification of non-poliomyelitis enteroviruses from material of patients was carried out according to WHO recommendations. Phenotyping and phylogenetic analysis of enteroviruses was carried out.

[Synthesis of extracellular phythohormones by Bacillus strains isolated from different ecological sources].

Synthesis of extracellular phytohormones by bacteria strains of the genus Bacillus isolated from different ecological sources (soil and plant) has been researched. It has been shown that phytohormone's synthesis ability of the endophytic and free-living Bacillus strains is significantly different as regards the level and spectrum of synthesized compounds. It was concluded that such differences in synthesis of extracellular phytohormones by bacilli are associated with different type of interactions between the studied microorganisms and host plant.

[Role of biocompatible adhesives in the increase of rhizobia physiological activity and productivity of soybean-rhizobia symbiosis].

On the basis of natural exopolysaccharide xanthan and exopolyacrylamide the sticky-gene composition has been developed. Addition of that composition to the culture medium provided a 26.3 times higher viability of Bradyrhizobium japonicum UCM B-6035 cells during its storage.

[Activity of main enzymes of ammonium assimilation in Bradyrhizobium japonicum under the influence of plant flavonoid inductors].

The activity of key enzymes of ammonium assimilation in cell-free extracts of soybean rhizobia characterized with different effectiveness in symbiosis, has been researched. It has been found out that at highly efficient strain Bradyrhizobium japonicum UKM B-6035 the most active links in the system of ammonium assimilation reactions are glutamate synthase (glutamate-oxoglutarate-aminotransferase, GOGAT) and glutamate dehydrogenase (GDH). Whereas the most active links at the ineffective strain B.

[Changes in external respiration function in response to berotek and histamine in diabetic patients].

Aim: To study airways reactivity in patients with diabetes mellitus (DM).

Material And Methods: Berotek and histamine tests were performed in 29 DM patients (type 1 DM - 20, type 2 DM - 9 patients) and 39 DM patients (12 and 27 patients, respectively). The age of the patients varied from 16 to 66 years.

[The impact of the social economic conditions on the reproduction processes].

The social economic conditions of population reproduction in the Trans-Dniester Region with the emphasis on its specifics are analyzed. The natural dynamics of population is estimated including gender and age peculiarities, marriage and divorces statistics, migration processes and population health in the region. It is shown that the population reproduction dynamics follows the natural laws and processes.

[Glutamate dehydrogenase activity of Bradyrhizobium japonicum in the presence of phytoregulators].

Influence of plant growth regulators ivin and emistim C, and flavonoids daidzein and quercetin on the glutamate dehydrogenase activity of soybean nodule bacteria, with contrasting symbiotic properties, were studied. It was shown that all used phytoregulators stimulated glutamate dehydrogenase activity of Bradyrhizobium japonicum 71t (the strain with highly efficient symbiotic properties) 1.2-4.

[Effect of plant growth regulators on physiological activity of Bradyrhizobium japonicum ].

Influence of plant growth regulators Ivin, Emistim C, Eney and Agrostimulin on the biomass production and exopolymers synthesis of soybean nodule bacteria, which have contrasting symbiotic properties, and glutamine synthetase activity of their cell-free extracts were studied. It was shown that the processes of the biomass and exopolymers accumulation had an opposite direction. Of all preparations only Ivin and Agrostimulin intensificol growth activity of the microorganisms under study.

[A model of the quinone-binding site of photosystem I: investigation of charge transfer complexes of phylloquinone and its derivatives with tryptophan].

Phylloquinone (vitamin K1; 2-methyl-3-phytyl-1,4-naphthoquinone), the secondary electron acceptor A1 in photosystem I of plants, algae, and cyanobacteria, mediates the electron transfer between A0 (a monomeric chlorophyll a) and the iron-sulfur cluster Fx. In order to investigate the interaction of vitamin K1 with the A1-binding site, their models on the non-covalent complexes were obtained and studied. The fluorescent properties of vitamin K1, its derivatives, and their complexes with Trp showed a possible role of amino acid components in the formation of a stable energy state, which provides the energy redistribution between oxidized and reduced forms of vitamin K1.