The impact of a second MRI and re-biopsy in patients with initial negative mpMRI-targeted and systematic biopsy for PIRADS ≥ 3 lesions.

Objective: To evaluate the proportions of detected prostate cancer (PCa) and clinically significant PCa (csPCa), as well as identify clinical predictors of PCa, in patients with PI-RADS >  = 3 lesion at mpMRI and initial negative targeted and systematic biopsy (initial biopsy) who underwent a second MRI and a re-biopsy.

Methods: A total of 290 patients from 10 tertiary referral centers were included. The primary outcome measures were the presence of PCa and csPCa at re-biopsy.

A Novel Smart Chair System for Posture Classification and Invisible ECG Monitoring.

In recent years, employment in sedentary occupations has continuously risen. Office workers are more prone to prolonged static sitting, spending 65−80% of work hours sitting, increasing risks for multiple health problems, including cardiovascular diseases and musculoskeletal disorders. These adverse health effects lead to decreased productivity, increased absenteeism and health care costs.

A Multidisciplinary Fingerprinting Approach for Authenticity and Geographical Traceability of Portuguese Wines.

The interest in developing reliable wine authenticity schemes is a hot-topic, especially for wines with recognized added-value. In order to accomplish this goal, two dimensions need to be considered: the grapevine variety determination and the geographical provenance. The aim of this study was to develop a multidisciplinary approach applicable to wines from the sub region Melgaço and Monção of the demarcated Vinho Verde region and from the demarcated Douro region.

Alternative SNP detection platforms, HRM and biosensors, for varietal identification in Vitis vinifera L. using F3H and LDOX genes.

The wine sector requires quick and reliable methods for Vitis vinifera L. varietal identification. The number of V.

Applying high-resolution melting (HRM) technology to olive oil and wine authenticity.

Olive oil and wine production have a worldwide economic impact. Their market reliability is under great concern because of the increasing number of fraud and adulteration attempts. The need for a traceability system in all its extension is crucial particularly for the cases of olive oils and wines with certified labels, in which only a limited number of olives and grapevine varieties, respectively, are allowed in a restricted well-defined geographical area.

Impaired Mental Health-Related Quality of Life in Patients with Idiopathic Ventricular Fibrillation.

Background: Idiopathic ventricular fibrillation (iVF) is diagnosed in cardiac arrest survivors without an identifiable cause. Data regarding the health-related quality of life (HRQoL) in iVF patients are lacking. The purpose of this study was to investigate the HRQoL of iVF patients and to compare it to patients with an implantable cardioverter defibrillator (ICD) diagnosed with an underlying disease and healthy subjects.

Characterization of neural network generalization in the determination of pH and anthocyanin content of wine grape in new vintages and varieties.

The generalization ability of hyperspectral imaging combined with neural networks (NN) in estimating pH and anthocyanin content during ripening was evaluated for vintages and varieties not employed in the NN creation. A NN, from a previously published work, trained with grape samples of Touriga Franca (TF) variety harvested in 2012 was tested with TF from 2013 and two new varieties, Touriga Nacional (TN) and Tinta Barroca (TB) from 2013. Each sample contained a small number of whole berries.

High Resolution Melting (HRM) applied to wine authenticity.

Wine authenticity methods are in increasing demand mainly in Denomination of Origin designations. The DNA-based methodologies are a reliable means of tracking food/wine varietal composition. The main aim of this work was the study of High Resolution Melting (HRM) application as a screening method for must and wine authenticity.

Biosensor for label-free DNA quantification based on functionalized LPGs.

A label-free fiber optic biosensor based on a long period grating (LPG) and a basic optical interrogation scheme using off the shelf components is used for the detection of in-situ DNA hybridization. A new methodology is proposed for the determination of the spectral position of the LPG mode resonance. The experimental limit of detection obtained for the DNA was 62±2nM and the limit of quantification was 209±7nM.

Vitis vinifera L. Single-Nucleotide Polymorphism Detection with High-Resolution Melting Analysis Based on the UDP-Glucose:Flavonoid 3-O-Glucosyltransferase Gene.

Vitis vinifera L. is a species with a large number of varieties, which differ in terms of anthocyanin content. The genes involved in the anthocyanin biosynthesis pathway have a direct effect in the anthocyanin profile of each variety, being potentially interesting for varietal identification.

Accessibility of Portuguese Public Universities' sites.

The aim of this study is to characterise the accessibility of all Public Portuguese Universities' homepages and the support services they offer to disabled students. We employed two different online automatic tools: using the 'eXaminator' to determine the conformance of websites to checkpoints; using 'HERA' to identify any automatically detectable errors or checkpoints. The choice of these tools was according to the Portuguese Government recommendation, to public in general, as the ones best suited to perform accessibility check-ups.

Genetic characterization of commercial Saccharomyces cerevisiae isolates recovered from vineyard environments.

One hundred isolates of the commercial Saccharomyces cerevisiae strain Zymaflore VL1 were recovered from spontaneous fermentations carried out with grapes collected from vineyards located close to wineries in the Vinho Verde wine region of Portugal. Isolates were differentiated based on their mitochondrial DNA restriction patterns and the evaluation of genetic polymorphisms was carried out by microsatellite analysis, interdelta sequence typing and pulsed-field gel electrophoresis (PFGE). Genetic patterns were compared to those obtained for 30 isolates of the original commercialized Zymaflore VL1 strain.

Functional analysis of Kluyveromyces lactis carboxylic acids permeases: heterologous expression of KlJEN1 and KlJEN2 genes.

The present work describes a detailed physiological and molecular characterization of the mechanisms of transport of carboxylic acids in Kluyveromyces lactis. This yeast species presents two homologue genes to JEN1 of Saccharomyces cerevisiae: KlJEN1 encodes a monocarboxylate permease and KlJEN2 encodes a dicarboxylic acid permease. In the strain K.