Publications by authors named "Leonie InderwiedenstraSSe"

Signaling of G protein-activated inwardly rectifying K (GIRK) channels is an important mechanism of the parasympathetic regulation of the heart rate and cardiac excitability. GIRK channels are inhibited during stimulation of G-coupled receptors (GPCRs) by depletion of phosphatidyl-4,5-bisphosphate (PIP) and/or channel phosphorylation by protein kinase C (PKC). The GPCR-dependent modulation of GIRK currents in terms of specific PKC isoform activation was analyzed in voltage-clamp experiments in rat atrial myocytes and in CHO or HEK 293 cells.

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Activation of a specific protein kinase C (PKC) isoform during stimulation of G protein-coupled receptors (GPCRs) is determined by homologous receptor desensitization that controls the spatiotemporal formation of downstream G signalling molecules. Furthermore, GPCR-activated PKC isoforms specifically regulate receptor activity via a negative feedback mechanism. In the present study, we investigated the contribution of several phosphorylation sites in the α-adrenergic receptor (α-AR) for PKC and G protein coupled receptor kinase 2 (GRK2) to homologous receptor desensitization and effector modulation.

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