Immune response against P815X2 mastocytoma growing in syngeneic DBA/2 mice. III. Morphometric assessment of the dynamic changes in post-capillary venules as regulatory elements of lymphocyte recirculation in tumor-draining lymph nodes.

To evaluate the dynamics of lymphocyte recirculation in tumor-bearing mice, the post-capillary venules (PCV) were subjected to quantitative measurements in the regional (RLN) and nonregional (NRLN) lymph nodes during the progression of P815X2 mastocytoma in syngeneic DBA/2 mice. Mice were sacrificed at two-day intervals, and RLNs and NRLNs were analysed for their content of B- and T-lymphocytes and their subsets, demonstrated by immunoperoxidase technique using monoclonal antibodies; Anti-Thy 1.2 (T cells), Anti-Lyt 1 (T-helper cells), Anti-Lyt 2 (T-suppressor cells), and Anti-I-Ad (B cell) antigens, separately in the B- and T-cell compartments.

Silicotic lymph node lesions in non-occupationally exposed lung carcinoma patients.

The report deals with a series of peribronchial and mediastinal lymph node silicotic lesions, which were histologically and histochemically typical, in patients with bronchogenic cancer without any form of occupational dust exposure. The presence of a significant quantity of quartz was confirmed in the lesions by the use of backscatter electron imaging analysis, X-ray mapping and X-ray spectrometry. The lymph node lesions observed were actually silicotic in nature and had possible epidemiologic implications.

Immune response against P815X2 mastocytoma growing in syngeneic DBA/2 mice. II. Morphometric assessment of immunoreactivity in the thymus and spleen related to lymphocyte subsets in peripheral blood.

To assess the significance of humoral immune mechanisms in host reactivity against the P815X2 mastocytoma grown in syngeneic DBA/2 mice, an approach was made to correlate immunomorphology of the thymus and spleen with the functional assays measuring cytotoxic (51Cr-release assay) and tumor cell membrane-bound antibodies (IF test) as well as phenotyping (with monoclonal antibodies Anti-Thy1.2, Anti-Lyt1, Anti-Lyt2, and Anti-I-Ad) the lymphocyte subsets in peripheral blood. The volume fractions (Vv) of the cortex (C), and medulla (M) of the thymus, as well as central (C-PALS) and peripheral periarteriolar lymphoid sheath (P-PALS) in the spleen were determined morphometrically.

Immune response against P815X2 mastocytoma growing in syngeneic DBA/2 mice. I. Morphometric assessment of lymph node immunoreactivity and analysis of circulating antibodies.

To assess the significance of humoral immune mechanisms in host reactivity against the P815X2 mastocytoma grown in syngeneic DBA/2 mice, an approach was made to correlate immunomorphology of the lymph nodes with the functional assays measuring cytotoxic and tumor cell membrane-bound antibodies in mouse sera. Regional and non-regional (RLN, NRLN) lymph nodes, were subjected to stereological analysis to determine the volume fractions (Vv) of the cortex (C), the paracortex (PCA), the germinal centers (GC), and the medulla (M), using a computerized analysis system (IBAS I, Kontron). In both RLN:s and NRLN:s, lymphocyte subsets were identified and their ratios determined using the ABC (avidin-biotin peroxidase complex) technique and following monoclonal antibodies; Anti-Thy 1.

Gastric malignant lymphoma: immunohistochemical findings correlated with histopathology and clinical data.

Nine cases of primary gastric lymphoma are reviewed. Clinical data are correlated with histopathology and immunohistochemical findings. The reported observations suggest a relationship between associated lesions (chronic gastritis, follicular gastritis) and primary gastric lymphoma.

Morphometry of lymphoid organs in health and disease.

Structural modification of lymph nodes in bronchogenic carcinoma, thymus cortex as a function of age, area and form factors of lymphoid cell nuclei in lymphomas and of epithelioid cell nuclei in tuberculosis and sarcoidosis have been measured by means of test point and/or image semiautomated analyzer.

Morphometric nuclear analysis of lymphoid cells in center cell lymphomas and in reactive germinal centers.

A comparison between neoplastic and nonneoplastic lymphoid cell nuclei within germinal centers of nodular lymphomas and of reactive follicular hyperplasias has been carried out with the help of a semiautomated image analyzer by measuring nuclear area, by assessing the presence or absence of invaginations, and by defining the form of the invagination. Nuclear areas are larger in lymphomas, where the invagination, if present, decreases in depth and increases in angle as the value of nuclear area becomes greater. Nuclei with such a large area and shallow invaginations are not present within the nonneoplastic germinal centers.

A morphometric semiautomated method for analyzing cell nuclei in lymph node sections from non-Hodgkin's lymphomas. Significance of data.

A semiautomated electronic system has been employed for sizing nuclear area and for evaluating nuclear form factors in non-Hodgkin's lymphomas with the purpose to correlate these parameters with survival and histotype. By mathematical models for best fits correlating the dependent variables with the survival has been demonstrated that an inverse correlation corresponding to a negative exponential function exists between mean nuclear area and survival. It has also been shown that the nuclear form is less irregular (i.

Scanning electron microscopy in the diagnosis of bronchogenic squamous cell and small cell anaplastic carcinoma.

The authors believe that differential diagnostic aspects between poorly-differentiated squamous cell carcinoma (PDS) and small cell anaplastic carcinoma (SCA) may be shown by scanning electron microscopy (SEM) analysis of 'thick' sections from routine paraffin-embedded tissues. In 8 'sample' cases features were demonstrated which were typical of PDS (well-defined outlines of cells, intercellular bridges) and of SCA (fairly smooth cell surface, nuclei in relief, a spongy, granular-like appearance of cytoplasm).

[The diagnosis of tubercular lymphadenitis in the case records of the Institute of Pathological Anatomy of Siena University in 1970-1981].

A study of the diagnosis of the tubercular lymphadenitis was carried out. No significant difference of incidence was observed through the years in the considered period. Tubercular lymphadenitis was more frequent in males under 15 years and in females over 51 years.

[Experimental chronic poisoning by mercury and alcohol: histopathologic, ultrastructural findings and toxicologic and enzymatic evaluations].

Rats were treated with HgCl2 and/or alcohol per os in a standard diet as to get a chronic intoxication. Histological and ultrastructural examination of the liver, GPT, GOT, LDH enzymatic activities, and toxicological mercury evaluation were performed. Liver cell degenerative changes with focal necrosis, and structural alteration and fibrosis were demonstrated in the group of rats treated with HgCl2.

Bronchogenic carcinoma: survival after surgical treatment according to stage, histologic type and immunomorphologic changes in regional lymph nodes.

The survival rates of 90 patients who underwent operation for bronchogenic carcinoma were analyzed statistically according to sex, age, tumor stage and histologic type, types of surgical procedures (lobectomy or pneumonectomy), and to immunomorphologic parameters of immunologic activity in peribronchial and hilar lymph nodes. The Stage I group survived significantly longer than did the Stage II and III groups, the lobectomized patients survived significantly longer than the pneumonectomized patients. The absence or presence of lymph node metastases was one of the major determinants of survival.

[Histopathology of the hepatic lesions in brucellosis based on biopsy findings].

22 liver biopsies from patients affected by brucellosis (Brucella melitensis) have been studied as far as granuloma formation is concerned. The first phase of granulomatous lesion seems to be the presence of monocytes within the hepatic sinusoids. Then we can observe small histiocytic granuloma and finally a "mature" (Adams [1]) granuloma, i.