The effect of diamide and glutathione on the uptake of glucose by human erythrocytes.

Diamide, directly added to human erythrocytes, inhibits glucose utilisation. The trend of this process is in good correlation with the intracellular concentration of GSH. Since diamide does not affect the glycolytic and hexose monophosphate-shunt-pathway enzymes, it is likely that the effect of diamide involves the sugar transport across erythrocyte membrane.

Studies on the inactivation of glyceraldehyde-3-phosphate dehydrogenase by methylglyoxal.

Glyceraldehyde-3-P dehydrogenase (E.C. 1.

Studies on the interaction of fructose 1,6-P2 aldolase with methylglyoxal.

Reaction of rabbit muscle fructose 1,6-P2 aldolase with methylglyoxal results in a biphasic loss of activity. The kinetics of the initial rapid phase are first order with respect to the inhibitor. Dihydroxyacetone phosphate and fructose 1,6 bisphosphate afford complete protection whereas inorganic phosphate provides only a partial protection against inactivation.

Comparative aspects of the inactivation of fructose 1,6 bisphosphate aldolase by D- and l-glyceraldehyde 3-phosphate.

Fructose 1,6-bisphosphate aldolase inactivation by L- and D-glyceraldehyde 3-phosphate (Ga 3-P) obeys pseudo first-order kinetics. L-Ga 3-P is much more effective than the D-isomer: the Ki values obtained are 0.032 mM and 0.

[Relationship between the platelet aggregation of the newborn and mother (author's transl)].

The platelets function of newborn aroused great interest in these last years. On this line, AA. made the stretching test according to Breddin K.

D,L-Glyceraldehyde 3-phosphate breakdown in the presence of selenalysine.

Selenalysine is more effective than lysine both on glyceraldehyde 3-P transformation into methylglyoxal and on subsequent methylglyoxal polymerization.

Characterization of the polymer formed from methylglyoxal in the presence of L(+)-lysine.

L(+)--lysine reacts with methylglyoxal in aqueous solution to give a yellow polymer. This polymer has been subjected to some chemical and physico-chemical characterizations. The gross structure of the polymer is probably formed by 3-hydroxypyrrolic nuclei, bridged together by vinylenic groups or -CH(OH)-CH2-CO-CH(OH)-groups.