Publications by authors named "Leonardo Uribe Castano"
We employ wide-field second harmonic generation (SHG) microscopy together with nonlinear Stokes polarimetry for quick ultrastructural investigation of large sample areas (700 μm × 700 μm) in thin histology sections. The Stokes vector components for SHG are obtained from the polarimetric measurements with incident and outgoing linear and circular polarization states. The Stokes components are used to construct the images of polarimetric parameters and deduce the maps of ultrastructural parameters of achiral and chiral nonlinear susceptibility tensor components ratios and cylindrical axis orientation in fibrillar materials.
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- The extracellular matrix (ECM) undergoes changes during cancer progression, but these morphological alterations are often overlooked in diagnostics.
- Polarimetric second-harmonic generation (P-SHG) microscopy enables detailed visualization of collagen structures in the ECM, improving understanding of cancer-induced changes.
- This study used P-SHG microscopy on a human lung tissue section to differentiate between normal and tumor-like tissues, creating a pseudo-color map that highlights ECM irregularities without relying on cell morphology.
Article Synopsis
- The extracellular matrix (ECM) collagen changes significantly during cancer development, but these changes are often overlooked in traditional cancer diagnostics, which typically use H&E staining.
- A new technique called polarimetric second-harmonic generation (P-SHG) microscopy allows for detailed imaging and analysis of collagen structures without the need for staining, providing more information about tissue characteristics.
- The study demonstrates that P-SHG microscopy can effectively differentiate between tumor and normal breast tissue, achieving high accuracy rates (94.2% accuracy) and suggesting it could be a valuable tool in cancer diagnostics and prognosis.