Publications by authors named "Leonardo M Moraes"
Article Synopsis
- Clomiphene citrate (CC) and letrozole are ovulatory stimulants associated with high ovulation rates but low pregnancy rates, prompting a study to evaluate their effects on apoptosis in human cumulus cells.
- The study involved primary cell cultures from IVF patients and utilized various assays to assess cell viability and gene expression; results showed that while these treatments did not significantly affect cell viability, letrozole reduced the percentage of cells in the S phase and CC increased certain gene expressions related to apoptosis.
- Overall, the findings suggest that CC and letrozole may influence cumulus cells at a cellular level and affect gene expression related to cell death, indicating potential direct effects beyond their traditional roles as ovulatory stimulants.
Acta Histochem
February 2021
Article Synopsis
- The study investigates the expression of pro-caspase-3 and cleaved caspase-3 in luteinized granulosa cells during in vitro fertilization.
- Pro-caspase-3 was found to be positive in 77% of cells, while cleaved caspase-3 was only present in 4%, indicating pro-caspase-3’s predominant role.
- The expression of pro-caspase-3 was consistent regardless of patient characteristics or CASP3 mRNA levels, suggesting it's always ready for activation to regulate apoptosis during ovarian stimulation.
