Human Hepatocyte 4-Acetoxy-,-Diisopropyltryptamine Metabolite Profiling by Reversed-Phase Liquid Chromatography Coupled with High-Resolution Tandem Mass Spectrometry.

Tryptamine intoxications and fatalities are increasing, although these novel psychoactive substances (NPS) are not controlled in most countries. There are few data on the metabolic pathways and enzymes involved in tryptamine biotransformation. 4-acetoxy-,-diisopropyltryptamine (4-AcO-DiPT) is a synthetic tryptamine related to 4-hydroxy-,-diisopropyltryptamine (4-OH-DiPT), 4-acetyloxy-,-dipropyltryptamine (4-AcO-DPT), and 4-acetoxy-,-dimethyltryptamine (4-AcO-DMT).