Early retinal differentiation of human pluripotent stem cells in microwell suspension cultures.

Objective: To develop a microwell suspension platform for the adaption of attached stem cell differentiation protocols into mixed suspension culture.

Results: We adapted an adherent protocol for the retinal differentiation of human induced pluripotent stem cells (hiPSCs) using a two-step protocol. Establishing the optimum embryoid body (EB) starting size and shaking speed resulted in the translation of the original adherent process into suspension culture.