Environmental Factors Modulate the Role of Sigma Factor in Clavulanic Acid Production in ATCC27064.

Sigma factors and sigma factor-related mechanisms control antibiotic production in . In this contribution, the gene was overexpressed in the wild-type strain of ATCC2764, yielding /pIORF21, to further evaluate its regulatory effect on clavulanic acid (CA) biosynthesis under different culture medium conditions. The overexpression, regulated under the constitutive promoter , led to 2.

Comparative Analysis of Strategies for Transcriptome Assembly in Prokaryotes: as a Case Study.

The performance of software tools for de novo transcriptome assembly greatly depends on the selection of software parameters. Up to now, the development of de novo transcriptome assembly for prokaryotes has not been as remarkable as that for eukaryotes. In this contribution, Rockhopper2 was used to perform a comparative transcriptome analysis of exposed to diverse environmental conditions.

Comparative Transcriptome Analysis of in Response to Favorable and Restrictive Nutritional Conditions.

Clavulanic acid (CA), a β-lactamase inhibitor, is industrially produced by the fermentation of . The efficiency of CA production is associated with media composition, culture conditions and physiological and genetic strain characteristics. However, the molecular pathways that govern CA regulation in remain unknown.

An enhanced genome-scale metabolic reconstruction of Streptomyces clavuligerus identifies novel strain improvement strategies.

In this work, we expanded and updated a genome-scale metabolic model of Streptomyces clavuligerus. The model includes 1021 genes and 1494 biochemical reactions; genome-reaction information was curated and new features related to clavam metabolism and to the biomass synthesis equation were incorporated. The model was validated using experimental data from the literature and simulations were performed to predict cellular growth and clavulanic acid biosynthesis.