Responses of 2 epiphytic yeasts to foliar infection by Rhizoctonia solani or mechanical wounding on the phylloplane of tall fescue.

A growth-chamber experiment was conducted to determine how foliar disease or wounding affects the ability of 2 phylloplane yeasts (Rhodotorula glutinis and Cryptococcus laurentii) to colonize leaves of tall fescue (Festuca arundinacea). Yeasts were applied separately and together onto healthy leaves, leaves infected with Rhizoctonia solani (diseased), and mechanically bruised (wounded) leaves. In all 3 trials, the leaf disturbance treatment significantly affected the abundance of yeast on the phylloplane of tall fescue.

Short-term temporal dynamics of yeast abundance on the tall fescue phylloplane.

Six replicate trials were conducted to determine the short-term temporal dynamics and the effects of foliar applications of nutrients on the phylloplane yeast community of tall fescue (Festuca arundinacea Schreb.). In each trial, 2% sucrose + 0.

The influence of exogenous nutrients on the abundance of yeasts on the phylloplane of turfgrass.

Four experiments were conducted to assess the effect of foliar applications of various nutrient solutions on the phylloplane yeast community of tall fescue (Festuca arundinacea Schreb.). In the first three experiments, increasing concentrations of sucrose (2-16%), yeast extract (0.

Variable adhesion and diurnal population patterns of epiphytic yeasts on creeping bentgrass.

Irrigation and an in vitro agitation assay were used to determine the percentage of the epiphytic yeast community (Cryptococcus, Pseudozyma, Rhodotorula, and Sporobolomyces) adhering to the phylloplane of creeping bentgrass (Agrostis palustris (Huds.) Pers.).

In vitro attachment of phylloplane yeasts to Botrytis cinerea, Rhizoctonia solani, and Sclerotinia homoeocarpa.

The ability of yeasts to attach to hyphae or conidia of phytopathogenic fungi has been speculated to contribute to biocontrol activity on plant surfaces. Attachment of phylloplane yeasts to Botrytis cinerea, Rhizoctonia solani, and Sclerotinia homoeocarpa was determined using in vitro attachment assays. Yeasts were incubated for 2 d on potato dextrose agar (PDA) prior to experimentation.

Effect of foliar disease on the epiphytic yeast communities of creeping bentgrass and tall fescue.

The effect of mechanical wounding or foliar diseases caused by Sclerotinia homoeocarpa or Rhizoctonia solani on the epiphytic yeast communities on creeping bentgrass and tall fescue were determined by leaf washing and dilution plating. Total yeast communities on healthy bentgrass and tall fescue leaves ranged from 7.9 x 103 to 1.

Sensitivity of Sclerotinia homoeocarpa Isolates to Propiconazole and Impact on Control of Dollar Spot.

In response to reports of reduced efficacy of propiconazole for control of dollar spot, isolates of Sclerotinia homoeocarpa were collected from several locations in Georgia and tested for sensitivity to propiconazole and other demethylation-inhibiting (DMI) fungicides. Two discriminatory concentrations of propiconazole (0.02 and 0.

The effects of fungicides on the phylloplane yeast populations of creeping bentgrass.

The effects of fungicides on population size and the development of fungicide resistance in the phylloplane yeast flora of bentgrass was investigated. In the spring of 2001, azoxystrobin, chlorothalonil, flutolanil, and propiconazole were applied separately over a 6-week period to creeping bentgrass (Agrostis palustris Huds.).