Sigma factors and sigma factor-related mechanisms control antibiotic production in . In this contribution, the gene was overexpressed in the wild-type strain of ATCC2764, yielding /pIORF21, to further evaluate its regulatory effect on clavulanic acid (CA) biosynthesis under different culture medium conditions. The overexpression, regulated under the constitutive promoter , led to 2.
View Article and Find Full Text PDFThe performance of software tools for de novo transcriptome assembly greatly depends on the selection of software parameters. Up to now, the development of de novo transcriptome assembly for prokaryotes has not been as remarkable as that for eukaryotes. In this contribution, Rockhopper2 was used to perform a comparative transcriptome analysis of exposed to diverse environmental conditions.
View Article and Find Full Text PDFClavulanic acid (CA), a β-lactamase inhibitor, is industrially produced by the fermentation of . The efficiency of CA production is associated with media composition, culture conditions and physiological and genetic strain characteristics. However, the molecular pathways that govern CA regulation in remain unknown.
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