Evaluation of Pyrophosphate-Driven Proton Pumps in under Stress Conditions.

In , pH homeostasis is reliant on ATP due to the use of proton-translocating ATPase (H-ATPase) which constitutes a major drain within cellular ATP supply. Here, an exogenous proton-translocating pyrophosphatase (H-PPase) from which uses inorganic pyrophosphate (PP) rather than ATP, was evaluated for its effect on reducing the ATP burden. The H-Ppase was localized to the vacuolar membrane or to the cell membrane, and their impact was studied under acetate stress at a low pH.