[Analysis of the species and quantitative composition of lactobacilli obtained from humans in a 300-day experiment with isolation].

Two hundred and two intestinal lactobacill strains were obtained and studied in a 30-d experiment with chambered human subjects. Species composition of intestinal lactobacilli varied in the course of the experiment. Species of lactobacilli persisting in organism in the period of isolation were identified.

[The quantitative composition of lactoflora and a method for its determination].

A newly developed method for the determination of the quantitative composition of lactoflora is described. According to this method, the material under study is subcultured, after its primary inoculation into solid selective medium M P C - 4, placed into fructose- and sorbitol-containing media by the replica method. The characterization of fecal lactoflora of young healthy persons, made with the use of the above-mentioned new method, is presented.

[Biological characteristics of strains of lactobacilli, promising for use as eubiotics].

In the course of in vitro studies 3 Lactobacillus strains with pronounced antagonistic activity against some pathogenic and opportunistic bacteria (shigellae, enteropathogenic Escherichia, Proteus, staphylococci) were selected. In experiments on germ-free rats faint colonization by L. plantarum 37 was observed in the small intestine, as well as in the large intestine when low doses of these bacilli were introduced into the gastrointestinal tract of the animals.

[The adhesive properties of bacteria of intestinal origin].

Differences between strains of nonpathogenic Escherichia and lactobacilli, as well as some pathogenic bacteria of enteric origin (Escherichia, Shigella, Campylobacter), in their capacity to adhesion to rat enteric and colonic cells have been shown in vitro. The strains under study have been found to possess more pronounced adhesiveness with respect to colonic cells, which is indicative of their higher receptive capacity in comparison with enteric cells. In the absence of normal microflora lactobacilli and Escherichia exhibit increased adhesiveness with respect to enteric cells.

[Determination of the quantitative composition of fecal microflora].

Shifts in the quantitative composition of fecal microflora may be assessed by estimating the summary share (in per cent) of the bacterial groups associated with fecal microbiocenosis, this share being estimated by the nonparametric statistics methods. The ratio between potentially dominating and concomitant groups of microorganisms in fecal microbiocenosis helps estimate the severity of disorders in the quantitative composition of fecal microflora.

[Species composition of intestinal lactoflora of healthy rhesus monkeys].

The results of the study of the species composition of the fecal microflora of 15 healthy rhesus macaques, from whom 204 strains of lactobacilli have been isolated and identified, are presented. The intestinal microflora has been shown to include different species, subspecies and biovars of homo- and heterofermentative lactobacilli and to possess definite individual properties. L.

[Reciprocal effect of Klebsiella pneumoniae and Lactobacillus on their cytadhesion].

Reciprocal effect of 4 strains of Klebsiella pneumoniae and 6 Lactobacillus strains on their cytadhesion in mixed populations was studied on a model of formalinized human erythrocytes. The Lactobacillus strains included 2 strains of Lactobacillus casei subsp. casei, 2 strains of L.

[Hemagglutinating and adhesive capacities of Klebsiella and Enterobacter strains].

The authors analyze the data of studies on the hemagglutinating and adhesive capacity of 290 cultures, including 118 K. pneumoniae strains and 64 E. cloacae strains isolated from sick children, as well as 59 K.

[Lactobacillus flora and colonization resistance].

Lactobacillus flora of healthy persons and the properties influencing the protective function of lactobacilli are described. It was shown that the species and quantitative composition of the Lactobacillus flora in healthy persons was characterized by individual features and the Lactobacillus flora of separate microbiotopes was different. Furthermore, the properties defining the protective function of lactobacilli in the host: colonization capacity, direct antimicrobial activity and interference with other protective mechanisms were also different in separate species and even strains.

[Enhancement of the phagocytic activity of cells of the mononuclear phagocytosing system by the peroral administration of lactobacilli].

The phagocytic function of the peritoneal exudate cells after oral and intraperitoneal administration of Lactobacillus casei subsp. casei, L. plantarum and L.

[Effect of antibiotics on the adhesive properties of microorganisms and an available model for its study].

The effect of benzylpenicillin, ampicillin, bicillin-3, carbenicillin, levomycetin, erythromycin, streptomycin and kanamycin on the adhesive properties of 9 test microbes including 5 strains of Lactobacillus from human microflora, 3 uropathornic strains of E. coli and 1 strain of S. aureus was studied with the method developed by the authors.

[Erythrocyte adhesiveness in cosmonauts].

The adhesive property of red blood cells of cosmonauts was investigated during various stages of their professional activity. The study was carried out using three test microorganisms: L. casei A6, L.

[Adhesive and hemagglutinating properties of lactobacilli].

The study of the adhesive and hemagglutinating properties of the strains of different Lactobacillus species isolated from the human digestive tract and sour milk products were carried out. 49 strains of 9 Lactobacillus species were studied; of these, 10 strains had been isolated from saliva, 11 strains from feces, 7 strains from milk and 5 strains from sour cream. 11 collection strains and 2 strains used in the production of lactobacterin served as controls.

[Intestinal mucosa of gnotobiotic rats administered lactobacilli].

When lactobacilli are administered to gnotobiotic rats (Fischer strain), a gradual increase of lymphocytic, eosinophilic and mast cell infiltration of the intestinal connective tissue stroma, dilatation of vessels and their filling with lymphocytes is observed. Radioautographically 7 months later, migration and differentiation of enterocytes in the crypta--villus system of the iliac mucous tunic increases. In the mesenteric lymph nodes the postcapillary venules increase in number, lymphocytes migrate out of them, focal plasmocytic reaction with presence of some blastic forms takes place.

[Lactobacillus flora of the digestive tract of the crews of Soiuz-13 and Saliut-4].

The species composition and biology of lactoflora of the saliva and feces of crewmembers from 8-, 30- and 63-day flights was investigated. Altogether 593 strains of lactobacilli were examined. The space flights did not cause significant changes in physiology, biochemistry, antagonistic properties or lysozyme activity of lactobacilli.

[Antibiotic sensitivity of Lactobacilli from the digestive tract of the crews of Soiuz-13 and Saliut-4].

Sensitivity to 19 antibiotics of 411 lactobacillus strains isolated from the saliva and feces of crewmembers who made 8-, 30- and 63-day flights was determined. Antibiotic sensitivity remained unchanged even in the 63-day flight. The antibiotics tested produced different effects on the lactoflora that varied from gentle--polymyxin and gentamycin, less gentle--neomycin and monomycin, to hazardous--penicillin and erythromycin, then rifampicin and levomycetin, and, finally, tetracycline and oleandomycin.

[Ability of lactobacilli in the human microflora to produce lysozyme].

Lysozyme activity of 403 strains of lactobacilli were investigated; of these 26 were from foreign sets and 377 were isolated from the contents of the stomach, feces and vaginal discharge of healthy adults. The species reference of lactobacillae was confirmed by the results of study of their physiologo-biochemical properties with the aid of 45 tests. A method of agar plates was adapted to determination of lysozyme: the autoclaved suspension of Micrococcus lysodeikticus (strain No.

[Electrophoretic characteristics of lactate dehydrogenase isoenzymes of lactobacilli and determination of their activity].

Lactate dehydrogenase isoenzymes of 10 museum strains of lactobacilli were examined electrophoretically in polyacrylamide gel. With respect to the relative electrophoretic motility and enzymic activity certain differences in the isoenzyme spectra of lactate dehydrogenase can give additional evidence for the identification of lactobacillar species.