[Subpopulations of peripheral blood neutrophils and lymphocytes in children with immune neutropenia].

Peripheral blood (PB) neutrophils and lymphocytes from 14 patients with immune neutropenia were studied using the technique of rosette-formation in 2 neutrophil fractions with diverse specific density. All the neutropenia cases were divided into 2 groups by phagocytosis completeness in all the PB neutrophil types studied. Patients of group 1 had impaired phagocytosis D-RFN in elevated levels of these cells, autorosette-forming lymphocytes and neutrophils.

[Transcobalamin II levels in blood plasma of children with acute leukemia].

Transcobalamin II (TcII) level was studied in plasma of 40 children with acute leukemia. TcII is a cobalamin-binding protein which mediated the cellular uptake of Cbl and interacted with surface membrane receptor of hemopoietic cells. Plasma TcII and cobalofilins were analysed by PAGE using 57Co-cyanocobalamin.

[Retrospective evaluation of blood immunologic parameters in the course of remission of acute lymphoblastic leukemia in children].

The authors investigated cellular and humoral immunity in 53 children over 3 years of age suffering from acute lymphoblastic leukemia. The children had remission lasting from 6 to 120 months and were followed up for 7-14 years after the diagnosis was made. The treatment was performed according to programs of polychemotherapy practiced in 1981-1988.

[Results of screening hematological and cytochemical blood studies of 906 children living in Bryansk Province at places with different intensities of soil contamination with cesium-137 and strontium-90].

Populations of children living in the Bryansk territory (radionuclide contamination 0.2-63.9 Cu/km2) are characterized by heterogeneous blood counts, though relevant mean values are close to control.

[The cytogenetic examination of different groups of children living in regions of Bryansk Province contaminated as a result of the Chernobyl accident].

The frequency of chromosome aberrations (CA) was studied in peripheral blood lymphocytes from healthy children and children with thyreopathology living in the city of Klintsy, Bryansk Province (contamination level up to 5 Ci/km2), and two Moscow groups, respectively. We have observed the elevated number of dicentrics and acentric fragments in cell from children with thyreopathology with respect to those from healthy children living in Klintsy. This fact cannot be explained by influence of disease because there was no difference in cytogenetical markers between the groups of healthy children and children with thyreopathology from Moscow.

[Changes in the activity of endonucleolysis of nuclear DNA in children with acute leukemia].

The previously established fact of low activity of Ca, Mg-dependent endonucleolysis of cell nucleus DNA in lymphoproliferative diseases (CME-activity) brought the authors to study intranuclear characteristics of lymphoid cells in childhood acute lymphoblastic leukemia (ALL). The intensity of DNA-endonucleolysis was measured in 0.7% agarose gel using electrophoresis.

[Monoclonal immunophenotyping of acute lymphoblastic leukemia cells in children using rapid immune alkaline phosphatase].

Monoclonal immunophenotyping of leukemia cells of the bone marrow was carried out by the method of rapid immune alkaline phosphatase (RIAP) in 30 patients with acute lymphoblastic leukemia (ALL) aged 6 months-14 years. The authors used a panel of monoclonal antibodies (MCA) produced by Leu (Belgium) and DAKO (Denmark) directed to antigens of differentiation clusters: Tdt, HLA-DR, CD: 10, 19, 20, 22, 7, 8, 2, 5, 13, 33, 14. The results indicate diversity of compositions of differentiating antigens on leukemia cells of the dominant population and a different degree of leukemic cell pool heterogenicity.

[Cytochemical lymphocytic markers as criteria suitable for mass and selective hematologic screening of children living in areas contaminated by radionuclides].

A cytochemical lymphocytogram by PAS--reaction and nonspecific esterase test was made using blood smears from 820 children living in different regions of the Bryansk area exposed to radioactive contamination and from 46 matched controls from noncontaminated territories. Most of the children were born before the Chernobyl accident. The cytochemical lymphocytogram was informative enough to evaluate lymphocytic pool and its renewal.

[Significance of morphological, cytochemical and immunological identification of tumor cells in acute leukemia in children].

The authors discuss the reported data and the results of own studies characterizing the current state of the problem of the phenotyping of leukemic cells. Emphasize the necessity of the use of a complex of morphological, cytochemical and immunological research methods for identification of tumor cells in children suffering from acute leukemia.

[Characteristics of lymphocyte populations in the bone marrow and peripheral blood of children with non-T cell acute lymphoblastic leukemia during remission].

Characteristics of lymphocyte populations in the bone marrow and peripheral blood were comparatively analyzed in children with non-T acute lymphoid leukemia (ALL) during remission, and in the reference group of children with conditionally normal hemopoiesis, as well as with population of lymphocytes obtained from the femur of 22-32-week fetuses. The analysis has shown pronounced changes in the children during remission. The shifts in the structure of lymphocyte population in the bone marrow of children during remission of non-T ALL are considered as compensatory, under conditions of a secondary immunodeficiency due to an abrupt diminution of the absolute number of bone marrow and circulating lymphocytes.

[Ag-negative phenomenon of nucleolar organizers of bone marrow and blood cells in children with acute lymphoblastic leukemia].

The activity of nucleoli-organizing (NOR's) regions was studied in leukemic cells of 53 children with immunologically typical ALL. The Ag-negative type of ALL was found in 9 cases of IA-ALL and Comm(+)-ALL. Dominating cells were not argentaffin.

[Two-dimensional size-charge distribution of bone marrow cells in children with chronic immune neutropenia].

Physical characteristics of bone marrow cells of normal donors were comparatively studied with those of children with immune neutropenia. As a result of the bone marrow cell separation in the density gradient according to their sedimentation rate, fractions enriched with cells of one histogenetic series (lymphoid, erythroid and granulocytic) were obtained. Electrophoretic mobility of immature granulocytes in normal donors differed from that in children with immune neutropenia.

[State of nucleolus-forming regions in bone marrow and blood cells in acute leukemia in children].

The study of the activity of nucleolus-forming areas in bone marrow and blood cells in 67 children with acute leukemia has shown the parameter variability depending on histogenetic affiliation, differentiation degree and on the proliferative activity of the cells.

[Evaluation of the pool of mature circulating lymphocytes in children with acute leukemia].

In children with acute leukemia, at the initial period of the disease the absolute number of T-lymphocytes is within the normal range, when the values of the total number of leucocytes is low or normal, and it is increased in cases with high initial leucocytosis. Dissociation of T-cell marker signs in the lymphocyte population has been revealed that evidences the presence of functionally defective T-cells in the circulation. The value of the relative content of E+ T-cells over 10% in the circulation is of prognostic significance.