Publications by authors named "Lenia Ferrao Beck"
We demonstrate that the "HOOF-Print" assay provides high power to discriminate among Brucella isolates collected on a small spatial scale (within Portugal). Additionally, we illustrate how haplotype identification using non-random association among markers allows resolution of B. melitensis biovars (1 and 3).
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- Swine brucellosis is caused by three Brucella suis biovars (1, 2, and 3), and current identification methods are often unreliable and labor-intensive.
- Researchers developed a multiplex PCR assay using a specific gene (omp2b) to categorize these biovars more effectively, revealing distinct amplification patterns for each biovar.
- The study found some field isolates labeled as biovars 2 or 3 still displayed patterns associated with biovar 1, indicating limitations in the new method's accuracy for typing B. suis.