Clinical value of nano-carbon lymphatic tracer for regional lymph node dissections of rectal cancer after neoadjuvant chemoradiotherapy.

Objectives: Regional lymph node (LN) volume decreases after neoadjuvant therapy, requiring a tracer for more accurate detection. Nano-carbon tracer is a third-generation tracer with several advantages, but its use for LN detection after neoadjuvant chemoradiotherapy for middle and low rectal cancer remains unclear. Therefore, this study investigated the effects and safety of anoscope-guided subrectal injections of nano-carbon suspension in this patient population.

The transcriptome difference between colorectal tumor and normal tissues revealed by single-cell sequencing.

The previous cancer studies were difficult to reproduce since the tumor tissues were analyzed directly. But the tumor tissues were actually a mixture of different cancer cells. The transcriptome of single-cell was much robust than the transcriptome of a mixed tissue.

Characteristics of fecal gut microbiota in patients with colorectal cancer at different stages and different sites.

Numerous studies have revealed that the gut microbiota serves an important role in the pathogenesis of colorectal cancer (CRC). The present study aimed to investigate the populations present in the gut microbiota in patients with CRC of different stages and at different sites. Fecal samples were obtained from 67 CRC patients and 30 healthy controls, which were analyzed by sequencing the V3-V4 region of the 16S rRNA gene.

A study of using carbon nanoparticles to improve lymph nodes staging for laparoscopic-assisted radical right hemicolectomy in colon cancer.

Purpose: This study aimed to analyze and evaluate the feasibility of using carbon nanoparticles (CNs) to track lymph nodes (LNs) metastases in right colon tumors, especially for patients who underwent laparoscopic-assisted radical right hemicolectomy.

Method: A total of 99 patients were enrolled in this retrospective study between November 2015 and September 2017 (control group n = 47). One day before surgery, 1 ml of CNs suspension was injected into the submucosal layer around the site of the primary lesions by colonoscopy.

Single-operator wire-guided cannulation technique enables easier cannulation of endoscopic retrograde cholangiopancreatography.

Background: The single-operator wire-guided cannulation technique in endoscopic retrograde cholangiopancreatography (ERCP) has been rarely reported.

Aims: This study was undertaken to determine the safety and efficiency of a single-operator wire-guided cannulation technique.

Methods: Four hundred sixty-five consecutive patients referred for ERCP were included in this prospective study and randomly divided into two groups.

Non-classical MHC-Ι genes in chronic hepatitis B and hepatocellular carcinoma.

The non-classical human leukocyte antigens (HLA)-E, HLA-G, and HLA-F have been shown to modulate immune responses. We examined whether non-classical HLA polymorphisms are associated with hepatitis B virus (HBV) infection and hepatocellular carcinoma (HCC). Fifteen Single-nucleotide polymorphisms (SNPs) in these non-classical class I alleles were investigated by ligase detection reaction.

The analysis of CD45 isoforms expression on HBV-specific T cells after liver transplantation.

This study aimed to analyze the expression of CD45 on HBV-specific T cells in patients who underwent liver transplantation (LT). We detected the distribution of two CD45 isoforms (CD45RA, CD45RO) and CD27 on peripheral blood lymphocytes including two HBV-specific pentamer cells by flow cytometry. Meanwhile, the levels of tacrolimus, plasma IL-7, and IL-15 were tested by ELISA.