[Some features of immunological status in young peoples irradiated in utero or in age up to 4 years as result of Chernobyl accident].

Some characteristics of immune system, namely quantities of serum immunoglobulins A, G, M and activity of free and hidden autoantibodies to DNA, cardiolipin and microsomal thyroid antigen were studied in young people irradiated in utero or in age up to 4 years through Chernobyl accident. The hallmarker of observed immunological changes is low content of immunoglobulin A. Degree of reduction was in back proportion with level 137Cs contamination inhabit territory.

Natural hidden autoantibodies react with negatively charged carbohydrates and xenoantigen Bdi.

Immunoglobulin preparations from sera of healthy donors contain polyspecific autoantibodies interacting with DNA and other charged antigens. These antibodies belong to the IgG class and can exist in the free or hidden state. The hidden antibody activity can be revealed after ion-exchange chromatography on QAE-Sephadex A-50.

Effect of natural antiphospholipid antibodies on antithrombotic activity of vascular wall.

Natural latent human antibodies cross-reacting with DNA and cardiolipin, interact with human endothelial cells, decrease antiaggregation activity of rat aortic wall, and increase fibrinolytic activity of the wall of the inferior vena cava. It is assumed that natural antiphospholipid antibodies present in immunoglobulin preparations in a latent state modify antithrombogenic activity of the vascular wall and are a potential cause of antiphospholipid syndrome.

Natural hidden antibodies reacting with DNA or cardiolipin bind to thymocytes and evoke their death.

Both free and hidden natural antibodies to DNA or cardiolipin were obtained from immunoglobulins of a normal donor. The free antibodies reacting with DNA or cardiolipin were isolated by means of affinity chromatography. Antibodies occurring in an hidden state were disengaged from the depleted immunoglobulins by ion-exchange chromatography and were then affinity-isolated on DNA or cardiolipin sorbents.

[Heterogeneity and avidity of autoantibodies reacting with DNA].

Subpopulations of auto-antibodies to DNA were isolated by affinity procedures from sera of two patients with SLE and from IgG of healthy donors after ion-exchange chromatography ("hidden" auto-antibodies). It was found that two subpopulations of auto-antibodies are present in SLE sera just as fractions of "hidden" auto-antibodies consist of three subpopulations. The dissociation constants were measured by ELISA for the interaction of all subpopulations of auto-antibodies to DNA.

["Masked" autoantibodies from the serum of healthy blood donors cross-reacting with DNA and bacterial lipopolysaccharides].

The "masked" autoantibodies with polyspecific activity were found in the serum of healthy donors. It is shown that these antibodies are able to react with antigens after the ion exchange chromatography with QAE-sephadex. It was found that these "masked" antibodies are able to cross-react with DNA and lipopolysaccharides of wide-spread species of bacteria.

[Interaction of immunoglobulins with charged biopolymers: significance in the regulation of humoral immunity].

When subjected to ion exchange chromatography on QAE-Sephadex A-50 or gel filtration of Sephadex G-200 under conditions that cause the dissociation of immune complexes at pH 4.05, immunoglobulins both from serum and its immunoglobulin fraction increase their interaction with charged antigens as native DNA and cardiolipin. Ion exchange chromatography also leads to the deaggregation of complexes.

[Effect of treatment with high doses of 6-methylprednisolone on the activity of anti-DNA antibodies and antiidiotypic activity in the sera of patients with systemic lupus erythematosus].

The effect of 6-methylprednisolone therapy at high doses on the time course of autoantibodies to DNA and anti-idiopathic (inhibitory) serum activity was studied in 22 patients suffering from SLE with a high clinicolaboratory activity. Pulse-therapy led to the improved status in 70% of the patients and rapid reduction of clinical and laboratory manifestations of disease (cerebrovasculitis, nephritis, skin involvement, etc.).

[Immunoglobulins acquire the ability to interact with DNA after chromatography on QAE-Sephadex].

The composition of IgG isolated from normal human serum includes a significant amount of antibodies interacting with native DNA. This ability of antibodies is revealed only after anion-exchange chromatography, resulting in the division of IgG into two fractions, i.e.

[Immunological aspects of pulse therapy in systemic lupus erythematosus].

Twenty SLE patients were examined against a background of pulse-therapy with methylprednisolone. The analysis showed that there was a decrease in the CIC concentration, antibodies to native DNA and an increase in the level of C3c and C4 components of the complement against a background of pulse-therapy. A more rapid time course of the CIC level was noted shortly after pulse-therapy as compared to changes in other immunological indices.

Biosynthesis and cytoplasmic distribution of small poly(A)-containing B2 RNA.

Previously, we described a small polyadenylated RNA predominantly located in cytoplasm and hybridizing with the ubiquitous B2 sequence of the mouse genome (Kramerov, D.A., Lekakh, I.

[Clinico-immunological characteristics of systemic lupus erythematosus with Raynaud and Sjögren's syndromes (report I)].

Forty-one patients suffering from chronic systemic lupus erythematosus with Raynaud and Sjögren's syndromes and 18 patients with an acute and subacute disease course were examined. It was established that chronic SLE was marked by a high frequency of antibodies to ribonucleoprotein, an increase in the concentration of IgA, the presence of rheumatoid factor. Although the decrease in the concentration of C3c and C4 components of complement was more demonstrable in patients with an acute or subacute course of SLE, the rate of demonstration of circulating immune complexes in the patients' groups under comparison was approximately the same.

[Nuclear and cytoplasmic RNA showing hybridization with repetitive sequences B1 and B2 of the mouse genome].

Heavy nuclear RNA contains high amounts of transcripts from repetitive sequences B1 and B2. Cytoplasmic poly(A+)RNA and in particular, polysomal poly(A)+RNA (mRNA) also contain these sequences though their content is much lower. An abundant 2 kb mRNA of mouse liver was found to contain B2 sequence.

The sequences homologous to major interspersed repeats B1 and B2 of mouse genome are present in mRNA and small cytoplasmic poly(A) + RNA.

Heavy nuclear RNA contains high amounts of transcripts from repetitive sequences B1 and B2. Cytoplasmic poly(A)+RNA and, particularly, polysomal poly(A)+RNA (mRNA) also include these sequences but in smaller amounts. The abundant 2 kb mRNA of mouse liver are found to have a B2 sequence.