Effects of feeding and aeration strategies on NO production and emission by an aerobic granular sludge system for municipal wastewater treatment.

The effects of a single feeding cycle followed by a continuous aeration phase (AND) and a step-feeding cycle followed by intermittent aerobic/idle phases (AND) on the production and emission of nitrous oxide (NO) from aerobic granular sludge (AGS) from real domestic sewage were studied. Higher NO emissions were observed in the AND treatment, and 9.2 ± 4.

Effects of a worksite labor gymnastics program on novice and experienced workers.

Introduction: Worksite labor gymnastics programs are interventions that can positively affect industrial workers. Studies show that experienced and novice workers perceive their working conditions differently. Our premise is that labor gymnastics programs affect these two groups diversely.

p14 forms meso-scale assemblies upon phase separation with NPM1.

NPM1 is an abundant nucleolar chaperone that, in addition to facilitating ribosome biogenesis, contributes to nucleolar stress responses and tumor suppression through its regulation of the p14 Alternative Reading Frame tumor suppressor protein (p14). Oncogenic stress induces p14 to inhibit MDM2, stabilize p53 and arrest the cell cycle. Under non-stress conditions, NPM1 stabilizes p14 in nucleoli, preventing its degradation and blocking p53 activation.

Extended conformations of bifurcating electron transfer flavoprotein constitute up to half the population, possibly mediating conformational change.

Electron transfer bifurcation enables biological systems to drive unfavourable (endergonic) electron transfer by coupling it to favourable (exergonic) transfer of a second electron. In electron transfer flavoproteins (ETFs), a domain-scale conformational change is believed to sever the favourable pathway after a single electron has used it, thereby preventing the energy dissipation that would accompany exergonic transfer of the second electron. To understand the conformation change that participates in turnover, we have deployed small-angle neutron scattering (SANS) and computational techniques to characterize the bifurcating ETF from (ETF).

Enhancing the Detection of Social Desirability Bias Using Machine Learning: A Novel Application of Person-Fit Indices.

Social desirability bias (SDB) is a common threat to the validity of conclusions from responses to a scale or survey. There is a wide range of person-fit statistics in the literature that can be employed to detect SDB. In addition, machine learning classifiers, such as logistic regression and random forest, have the potential to distinguish between biased and unbiased responses.

Selective deuteration of an RNA:RNA complex for structural analysis using small-angle scattering.

The structures of RNA:RNA complexes regulate many biological processes. Despite their importance, protein-free RNA:RNA complexes represent a tiny fraction of experimentally-determined structures. Here, we describe a joint small-angle X-ray and neutron scattering (SAXS/SANS) approach to structurally interrogate conformational changes in a model RNA:RNA complex.

Statistical power and optimal design for randomized controlled trials investigating mediation effects.

Mediation analyses in randomized controlled trials (RCTs) can unpack potential causal pathways between interventions and outcomes and help the iterative improvement of interventions. When designing RCTs investigating these mechanisms, two key considerations are (a) the sample size needed to achieve adequate statistical power and (b) the efficient use of resources. The current study has developed closed-form statistical power formulas for RCTs investigating mediation effects with and without covariates under the Sobel and joint significance tests.

SANS investigation of fungal loosenins reveal substrate dependent impacts of protein 1 action on inter-fibril distance and packing order of cellulosic substrates.

Background: Microbial expansin-related proteins include fungal loosenins, which have been previously shown to disrupt cellulose networks and enhance the enzymatic conversion of cellulosic substrates. Despite showing beneficial impacts to cellulose processing, detailed characterization of cellulosic materials after loosenin treatment is lacking. In this study, small-angle neutron scattering (SANS) was used to investigate the effects of three recombinantly produced loosenins that originate from Phanerochaete carnosa, PcaLOOL7, PcaLOOL9, and PcaLOOL12, on the organization of holocellulose preparations from Eucalyptus and Spruce wood samples.

Analysis of the influence of occupational, sociodemographic and health factors on the demotivation of the intensivist.

Introduction: Understanding motivation, identifying motivational factors of health professionals, and recognizing how managers and leaders can successfully motivate healthcare professionals is a growing concern.

Objectives: To assess the occupational, sociodemographic, and health factors that influence the occurrence of demotivation in the intensive care unit professionals.

Methods: We performed a cross-sectional study with health professionals from nine intensive care units in João Pessoa, Paraíba state, Brazil.

Influence of extended working hours and physical recovery on absenteeism in the footwear industry from a system dynamics model.

. Work-related musculoskeletal disorders (WMSDs) are recurrent in the footwear industry, resulting in absenteeism. This study aimed to quantitatively analyze the influence of overtime work and physical recovery time on the occurrence of WMSD-related absenteeism using a system dynamics model.

Volatile fatty acid and methane production from vinasse and microalgae using two-stage anaerobic co-digestion.

The effects of adding vinasse (VIN) as a co-substrate on the stability and production of volatile fatty acids (VFA) and methane (CH) during the anaerobic digestion (AD) of microalgal biomass (MB) were evaluated. The AD system consisted of an acidogenic reactor (AR) followed by a methanogenic reactor (MR). The experiment was divided into phase I-start-up and AD of VIN; phase II-MB+VIN co-digestion (50:50 based on chemical oxygen demand (COD)); and phase III-co-digestion of pretreated MB and VIN (PTMB+VIN, 50:50).

A comparison of random forest-based missing imputation methods for covariates in propensity score analysis.

Propensity score analysis (PSA) is a prominent method to alleviate selection bias in observational studies, but missing data in covariates is prevalent and must be dealt with during propensity score estimation. Through Monte Carlo simulations, this study evaluates the use of imputation methods based on multiple random forests algorithms to handle missing data in covariates: multivariate imputation by chained equations-random forest (Caliber), proximity imputation (PI), and missForest. The results indicated that PI and missForest outperformed other methods with respect to bias of average treatment effect regardless of sample size and missing mechanisms.

Partial nitrification and simultaneous denitrification in sequential anaerobic and aerobic reactors: performance and microbial community dynamics.

The removal of organic matter and nitrogen from domestic sewage was evaluated using a system composed of two sequential reactors: an anaerobic reactor (ANR) with suspended sludge and an aerobic (AER) reactor with suspended and adhered sludge to polyurethane foams. Nitrogen removal consisted of AER operating at low dissolved oxygen (DO) concentrations; this favoured the simultaneous nitrification and denitrification (SND) process. The concentration of COD and N were 440 mgO.

SANS reveals lipid-dependent oligomerization of an intramembrane aspartyl protease from H. volcanii.

Reactions that occur within the lipid membrane involve, at minimum, ternary complexes among the enzyme, substrate, and lipid. For many systems, the impact of the lipid in regulating activity or oligomerization state is poorly understood. Here, we used small-angle neutron scattering (SANS) to structurally characterize an intramembrane aspartyl protease (IAP), a class of membrane-bound enzymes that use membrane-embedded aspartate residues to hydrolyze transmembrane segments of biologically relevant substrates.

Biomolecular condensates form spatially inhomogeneous network fluids.

The functions of biomolecular condensates are thought to be influenced by their material properties, and these will be determined by the internal organization of molecules within condensates. However, structural characterizations of condensates are challenging, and rarely reported. Here, we deploy a combination of small angle neutron scattering, fluorescence recovery after photobleaching, and coarse-grained molecular dynamics simulations to provide structural descriptions of model condensates that are formed by macromolecules from nucleolar granular components (GCs).

Anaerobic co-digestion of microalgal biomass, sugarcane vinasse, and residual glycerol from biodiesel using simplex-centroid mixture design: methane potential, synergic effect, and microbial diversity.

Microalgal biomass (MB) is a promising feedstock for bioenergy production. Nonetheless, the cell recalcitrance and the low C/N ratio limit the methane yield during anaerobic digestion. As an alternative to overcome these challenges, MB co-digestion with different feedstocks has been proposed.

Simple Scattering: Lipid nanoparticle structural data repository.

Lipid nanoparticles (LNPs) are being intensively researched and developed to leverage their ability to safely and effectively deliver therapeutics. To achieve optimal therapeutic delivery, a comprehensive understanding of the relationship between formulation, structure, and efficacy is critical. However, the vast chemical space involved in the production of LNPs and the resulting structural complexity make the structure to function relationship challenging to assess and predict.

Influence of environmental variables on students' cognitive performance in indoor higher education environments.

Background: Educational environments can have environmental conditions that are incompatible with the needs of students, compromising their well-being and affecting their performance.

Objective: To identify the environmental variables that influence the performance of university students and measure this influence through an experiment in indoor environments.

Methods: The study applied an experimental methodology for three consecutive days in seven educational environments located in different regions of Brazil, measuring the environ-mental conditions, the students' perception of the environment, and their cognitive performance.

Morphological Characterization of Self-Amplifying mRNA Lipid Nanoparticles.

The mRNA technology has emerged as a rapid modality to develop vaccines during pandemic situations with the potential to protect against endemic diseases. The success of mRNA in producing an antigen is dependent on the ability to deliver mRNA to the cells using a vehicle, which typically consists of a lipid nanoparticle (LNP). Self-amplifying mRNA (SAM) is a synthetic mRNA platform that, besides encoding for the antigen of interest, includes the replication machinery for mRNA amplification in the cells.

Biomolecular condensates form spatially inhomogeneous network fluids.

The functions of biomolecular condensates are thought to be influenced by their material properties, and these are in turn determined by the multiscale structural features within condensates. However, structural characterizations of condensates are challenging, and hence rarely reported. Here, we deploy a combination of small angle neutron scattering, fluorescence recovery after photobleaching, and bespoke coarse-grained molecular dynamics simulations to provide structural descriptions of model condensates that mimic nucleolar granular components (GCs).

Biomolecular condensates form spatially inhomogeneous network fluids.

The functions of biomolecular condensates are thought to be influenced by their material properties, and these will be determined by the internal organization of molecules within condensates. However, structural characterizations of condensates are challenging, and rarely reported. Here, we deploy a combination of small angle neutron scattering, fluorescence recovery after photobleaching, and coarse-grained molecular dynamics simulations to provide structural descriptions of model condensates that are formed by macromolecules from nucleolar granular components (GCs).

Acute outcomes of acupuncture and dry needling over peripheral acute fatigue in untrained healthy volunteers: A randomized controlled clinical trial.

Peripheral acute fatigue (PAF) is defined as when the skeletal muscle is incapable of generating power. We aimed to investigate the acute effects of traditional Chinese acupuncture (TCA) and dry needling (DN) over PAF induced on the biceps brachii of untrained healthy volunteers. We conducted a randomized, single-blind controlled clinical trial.

Methods for extraction, isolation and sequencing of cyclotides and others cyclic peptides with anti-helminthic activities: An overview.

The mortality rate caused by parasitic worms on their hosts is of great concern and studies have been carried out to find molecules to reduce the prevalence, host-parasite interaction, and resistance of parasites to treatments. Existing drugs on the market are very often toxic and have many side effects, hence the need to find new, more active molecules. It has been demonstrated in several works that medicinal plants constitute a wide range of new molecules that can solve this problem.

A Comparison of Person-Fit Indices to Detect Social Desirability Bias.

Social desirability bias (SDB) has been a major concern in educational and psychological assessments when measuring latent variables because it has the potential to introduce measurement error and bias in assessments. Person-fit indices can detect bias in the form of misfitted response vectors. The objective of this study was to compare the performance of 14 person-fit indices to identify SDB in simulated responses.