The complete mitochondrial DNA of the carnivorous sponge is putatively complemented by microDNAs.

Carnivorous sponges (Porifera, Demospongiae, Cladorhizidae), contrary to the usual filter-feeding mechanism of sponges, are specialized in catching larger prey through adhesive surfaces or hook-like spicules. The mitochondrial DNA of sponges overall present several divergences from other metazoans, and while presenting unique features among major transitions, such as in calcarean and glass sponges, poriferan mitogenomes are relatively stable within their groups. Here, we report and discuss the mitogenome of (Vacelet & Boury-Esnault, 1996), which greatly vary from its subordinal counterparts in both structure and gene order.

Micropropagation and in vitro rejuvenation of F. Muell.

Micropropagation is an important tool for the propagation for recalcitrant species, like This is a first reported about a complete study of the , it includes the vegetative rescue protocol of mature trees, its micropropagation up to the implementation of a mini-garden and clonal micro-garden and evaluation of adventitious rooting of their propagules. The in vitro rejuvenation of genotypes (03, 05, 06, 13 and 15), over 21 successive subcultures, was evaluated by comparing minicutting and microcutting techniques and the efficiency of this biotechnological tool for cloning of the mature trees. The results provide evidence of the in vitro rejuvenation occurring in the mature trees during micropropagation, leading to increases in the multiplication ratios of the microstumps of three genotypes (03, 05 and 15).

Anticonvulsant, Anticholinesterase and Cytoprotective Effects of the Aqueous Extract of Cham.

(1) Background: Cham is a Brazilian aromatic plant rich in phenolic compounds. In traditional medicine, its leaves are used to treat diseases of the Central Nervous System such as stress and anxiety. This study evaluates the capacity of the aqueous extract of as an anticonvulsant, anticholinesterase and antihemolytic agent.

Ponatinib: A Review of the History of Medicinal Chemistry behind Its Development.

The primary treatment for chronic myeloid leukemia (CML) involves first- and second-generation tyrosine kinase inhibitors (TKIs), such as imatinib, nilotinib, bosutinib, and dasatinib. However, these medications are ineffective against mutations in the kinase domain of the ABL1 protein, particularly in the protein with the T315I mutation. To address this, ponatinib (PNT), a third-generation inhibitor, was developed.

Similarity and economy of scale in urban transportation networks and optimal transport-based infrastructures.

Designing and optimizing the structure of urban transportation networks is a challenging task. In this study, we propose a method inspired by optimal transport theory and the principle of economy of scale that uses little information in input to generate structures that are similar to those of public transportation networks. Contrarily to standard approaches, it does not assume any initial backbone network infrastructure but rather extracts this directly from a continuous space using only a few origin and destination points, generating networks from scratch.

Lipid-Lowering and Anti-Inflammatory Effects of Leaves in Adipocytes and .

Obesity is a pandemic disease characterized by lipid accumulation, increased proinflammatory cytokines, and reactive oxygen species. It is associated with the development of comorbidities that lead to death. Additionally, drug treatments developed to control obesity are insufficient and have a variety of adverse effects.

Identification of Enterococcus spp. by MALDI-TOF mass spectrometry isolated from clinical mastitis and bulk tank milk samples.

Background: Throughout a three-year study period, 1,577 bovine clinical mastitis samples and 302 bulk tank samples were analyzed from ten Brazilian dairy herds. Enterococcus spp. was isolated and identified in 93 (5.

Genipa americana L.: A Review on Traditional Uses, Phytochemistry and Biological Activities.

Genipa americana L. (Rubiaceae), genip tree, has therapeutic and nutritional potential. This revision aimed to gather information on botanical characteristics, popular uses, phytochemical, and pharmacological aspects of the tree.

From chaos to clarity: The scientometric breakthrough in COVID-19 research.

Background: The COVID-19 pandemic paralyzed the world for over three years, generating unprecedented social changes in recent human history.

Aims: We aimed to scientometrically summarize a global and temporal overview of publications on COVID-19 in the two worst years of the pandemic and its progression in early 2022, after the start of vaccination.

Methods: Using the Web of Science database, this review covered the period from late 2019 to March 2022 and included all publications identified using the following terms: "SARS-CoV-2", "COVID-19", "Coronavirus Disease 19", and "2019-nCoV".

Nursing terminology for the care of people with respiratory diseases and Covid-19.

Objectives: To build a specialized nursing terminology for the care of people with respiratory diseases and Covid-19 or who have respiratory diseases after Covid-19, based on ICNP®.

Method: Methodological study developed in two stages: (1) identification of the relevant concepts for the health priority chosen from the literature; (2) cross-mapping of the identified concepts with the concepts contained in ICNP® version 2019/2020.

Results: 9460 terms were extracted from the literature, of which 4065 terms were excluded because they were not related to the object of study and 5395 were submitted to the mapping technique, resulting in 290 constant terms in the ICNP® and 5134 non-constant terms.

An atlas of spider development at single-cell resolution provides new insights into arthropod embryogenesis.

Spiders are a diverse order of chelicerates that diverged from other arthropods over 500 million years ago. Research on spider embryogenesis, particularly studies using the common house spider Parasteatoda tepidariorum, has made important contributions to understanding the evolution of animal development, including axis formation, segmentation, and patterning. However, we lack knowledge about the cells that build spider embryos, their gene expression profiles and fate.

Spirooxadiazoline-oxindoles derived from imatinib show antimyeloproliferative potential in K562 cells.

Imatinib mesylate was the first representative BCR-ABL1 tyrosine kinase inhibitor (TKI) class for the treatment of chronic myeloid leukemia. Despite the revolution promoted by TKIs in the treatment of this pathology, a resistance mechanism occurs against all BCR-ABL1 inhibitors, necessitating a constant search for new therapeutic options. To develop new antimyeloproliferative substances, we applied a medicinal chemistry tool known as molecular hybridization to design 25 new substances.

An acute bout of resistance exercise increases BDNF in hippocampus and restores the long-term memory of insulin-resistant rats.

A sedentary lifestyle, inadequate diet, and obesity are substantial risk factors for Type 2 diabetes mellitus (T2DM) development. A major picture of T2DM is insulin resistance (IR), which causes many impairments in brain physiology, such as increased proinflammatory state and decreased brain-derived neurotrophic factor (BDNF) concentration, hence reducing cognitive function. Physical exercise is a non-pharmacological tool for managing T2DM/IR and its complications.

Effect of 12-week high-intensity interval training on hemodynamic variables at rest and during exercise in patients with obstructive sleep apnoea.

This study aimed to investigate the impact of 12 weeks of high-intensity interval training (HIIT) on hemodynamic variables at rest and during exercise in patients with obstructive sleep apnoea. Twenty-six obese adults with moderate-to-severe OSA (AHI = 42 ± 22.9 e/h) were randomly assigned to HIIT or a control group.

Novel Energetic Co-Reactant for Thermal Oxide Atomic Layer Deposition: The Impact of Plasma-Activated Water on AlO Film Growth.

In the presented study, a novel approach for thermal atomic layer deposition (ALD) of AlO thin films using plasma-activated water (PAW) as a co-reactant, replacing traditionally employed deionized (DI) water, is introduced. Utilizing ex situ PAW achieves up to a 16.4% increase in the growth per cycle (GPC) of AlO films, consistent with results from plasma-enhanced atomic layer deposition (PEALD).

New azaaurone derivatives as potential multitarget agents in HIV-TB coinfection.

Tuberculosis (TB) disease, caused by Mycobacterium tuberculosis (Mtb) is the leading cause of death among people with human immunodeficiency virus (HIV) infection. No dual-target drug is currently being used to simultaneously treat both infections. This work aimed to obtain new multitarget HIV-TB agents, with the goal of optimizing treatments and preventing this coinfection.

Effect of high-intensity interval training on obstructive sleep apnea severity: A randomized controlled trial.

Vigorous physical activity has been associated with a reduced risk of developing obstructive sleep apnea (OSA). However, whether high-intensity interval training (HIIT) reduces OSA severity remains unclear. Thus, this study aimed to investigate the impact of 12 weeks of HIIT on the apnea-hypopnea index (AHI) and sleep parameters in participants with moderate-severe OSA.

Evolution of the Spider Homeobox Gene Repertoire by Tandem and Whole Genome Duplication.

Gene duplication generates new genetic material that can contribute to the evolution of gene regulatory networks and phenotypes. Duplicated genes can undergo subfunctionalization to partition ancestral functions and/or neofunctionalization to assume a new function. We previously found there had been a whole genome duplication (WGD) in an ancestor of arachnopulmonates, the lineage including spiders and scorpions but excluding other arachnids like mites, ticks, and harvestmen.

Rapid response of a public health reference laboratory to the COVID-19 pandemic.

Brazil was one of the most affected countries by the COVID-19 pandemic. Instituto Adolfo Lutz (IAL) is the reference laboratory for COVID-19 in São Paulo, the most populous state in Brazil. In April 2020, a secondary diagnostic pole named IAL-2 was created to enhance IAL's capacity for COVID-19 diagnosis.

Isotope Dilution Analysis for Particle Mass Determination Using Single-Particle Inductively Coupled Plasma Time-of-Flight Mass Spectrometry: Application to Size Determination of Silver Nanoparticles.

This paper describes methodology based on the application of isotope dilution (ID) in single-particle inductively coupled plasma time-of-flight mass spectrometry (spICP-ToFMS) mode for the mass determination (and sizing) of silver nanoparticles (AgNPs). For this purpose, and considering that the analytical signal in spICP-MS shows a transient nature, an isotope dilution equation used for online work was adapted and used for the mass determination of individual NPs. The method proposed measures NP isotope ratios in a particle-to-particle approach, which allows for the characterization of NP mass (and size) distributions and not only the mean size of the distribution.

Single-cell atlases of two lophotrochozoan larvae highlight their complex evolutionary histories.

Pelagic larval stages are widespread across animals, yet it is unclear whether larvae were present in the last common ancestor of animals or whether they evolved multiple times due to common selective pressures. Many marine larvae are at least superficially similar; they are small, swim through the beating of bands of cilia, and sense the environment with an apical organ. To understand these similarities, we have generated single-cell atlases for marine larvae from two animal phyla and have compared their cell types.

Checklist, diversity descriptors and selected descriptions of a highly diverse intertidal sponge (Porifera) assemblage at Costa do Descobrimento (Bahia, Brazil).

Costa do Descobrimento is located in southern Bahia, Brazil, and only 22 species of sponges (Porifera) were known from the area until now, despite its important reef formations. In the present study, we report a checklist of the sponges of Costa do Descobrimento and their distribution in the studied reefs, with several species illustrated in life. We also describe nine new sponge records for this region, some of which are new records for Brazil, or new records of species previously only poorly known.