Publications by authors named "Leila Saba"
Article Synopsis
- - Immunoprecipitation (IP) paired with mass spectrometry is a powerful method for mapping how proteins interact, especially when using tagged cell collections, but many protein interactions remain unexplored due to limitations in current high-throughput techniques.
- - A major challenge in IP is maintaining stable interactions when transferring samples, as this can result in losing real interactions and failing to identify them later.
- - The authors developed a high-content screening approach that investigates how different chemical conditions affect IP results, allowing for quick optimization of methods to better capture protein complexes.
Methods Mol Biol
December 2022
Article Synopsis
- LINE-1 (L1) retrotransposons create diverse ribonucleoproteins (RNPs) during their replication and the host's efforts to limit them, but their exact makeup is still not well understood.
- The composition of L1-associated macromolecules varies according to factors like the L1 life cycle stage, whether it’s dealt with productively or suppressed, and the specific cell type involved.
- This chapter presents techniques for identifying and analyzing the protein and RNA components of L1 macromolecules from tissues, using embryonal carcinoma cell lines (like N2102Ep) and colorectal cancer tissues as primary examples.
