Preparation of polymeric particles in CO medium using non-toxic solvents: discussions on the mechanism of particle formation.

The aim of this work was to develop a novel formulation method, termed modified-PGSS (modified-Particle from Gas Saturated Solution), for the encapsulation of protein into polymeric microparticles in CO medium. In this study, isosorbide dimethyl ether (DMI), a non-toxic water-miscible solvent, was used for the formulation and lysozyme was chosen as a model protein for encapsulation into PLGA microparticles. First, the mechanism of particle formation has been extensively studied and was discussed in detail.

Lysozyme encapsulation into nanostructured CaCO microparticles using a supercritical CO process and comparison with the normal route.

The aim of the present work was to assess the merits of supercritical CO (SC-CO) as a process for protein encapsulation into calcium carbonate microparticles. Lysozyme, chosen as a model protein, was entrapped during CaCO precipitation in two different media: water (normal route) and SC-CO. The particles were characterized and compared in terms of size, zeta potential, morphology by SEM, crystal polymorph and lysozyme encapsulation.

Auto-associative amphiphilic polysaccharides as drug delivery systems.

Self-assembly of amphiphilic polysaccharides provides a positive outlook for drug delivery systems without the need for solvents or surfactants. Various polymeric amphiphilic polysaccharides undergo intramolecular or intermolecular associations in water. This type of association, promoted by hydrophobic segments, led to the formation of various drug delivery systems such as micelles, nanoparticles, liposomes and hydrogels.