Identification of management units in threatened populations of Arnica mallotopus Makino (Asteraceae) using novel EST-SSR markers.

Arnica mallotopus is a perennial herb endemic to the snowy regions of Japan. At the southern edge of its distribution, in Kyoto Prefecture, overgrazing by sika deer and decreased snowfall have resulted in the rapid decline of A. mallotopus populations.

Development of nuclear SSRs for the insular shrub Clerodendrum izuinsulare (Verbenaceae) and the widespread C. trichotomum.

Premise Of The Study: Nuclear microsatellite primers were developed in the insular shrub Clerodendrum izuinsulare and the closely related widespread C. trichotomum to provide molecular tools to undertake a comparative study of the reproductive systems and genetic structures of the two Clerodendrum species.

Methods And Results: Using an improved protocol for isolating codominant compound microsatellite markers, 19 primer sets were developed in C.