Simultaneous extraction and determination of free and conjugated phytosterols in tobacco.

Acid hydrolysis and alkaline saponification were incorporated into a microwave-assisted extraction process for the simultaneous extraction of free and conjugated phytosterols from tobacco. The crude extract of the microwave-assisted extraction was purified by C18 solid-phase extraction and then determined by high-performance liquid chromatography. Phytosterols of cholesterol, ergosterol, stigmasterol, campesterol, and β-sitosterol were determined by chromatographic quantification.

Determination of gouty arthritis' biomarkers in human urine using reversed-phase high-performance liquid chromatography.

Creatinine, uric acid, hypoxanthine and xanthine are important diagnostic biomarkers in human urine for gouty arthritis or renal disease diacrisis. A simple method for simultaneous determination of these biomarkers in urine based on reversed-phase high-performance liquid chromatography (RP-HPLC) with ultraviolet (UV) detector was proposed. After pretreatment by dilution, centrifugation and filtration, the biomarkers in urine samples were separated by ODS-BP column by elution with methanol/50 mM NaHPO buffer solution at pH 5.